Functional Analysis of Protein Targets by Metabolomic Approaches

Abstract

Proteomics methods, such as activity-based protein profiling, can be used to connect proteins to biology and disease. Some proteins found through unbiased methods are not well characterized, which makes it difficult to ascertain the role of these proteins. Metabolomics approaches are useful in characterizing proteins that regulate or bind metabolites. Here, we provide examples of the development and use of metabolomics approaches to elucidate protein-metabolite interactions.

Activity-Based Protein Profiling

“…Specific protein targets of activity-based labeling were confirmed by heat-or pH-dependence in their labeling patterns [162]. To explore this untested electrophilic group in a proteomic setting, Cravatt and coworkers created a library of sulfonate ester probes equipped with either a biotin or a rhodamine tag.…”