Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants

Lin, Yongwen; Lin, Senjie; Guo, Hai Peng; Zhang, Zhiyi; Chen, Xiaoyang

doi:10.1007/s10529-013-1226-2

Cited by 33 publications

(23 citation statements)

References 10 publications

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“…Moreover, these plants have activated the expression of stress-related genes. Therefore, these data clearly show the regulatory function of G6PDH during low temperature stress (Lin et al, 2013).…”

Section: Function Of Nadp-dehydrogenases Under Environmental Stress Csupporting

confidence: 57%

NADPH-generating dehydrogenases: their role in the mechanism of protection against nitro-oxidative stress induced by adverse environmental conditions

Corpas

Barroso

2014

Front. Environ. Sci.

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NADPH is an essential reductive coenzyme in biosynthetic processes such as cell growth, proliferation, and detoxification in eukaryotic cells. It is required by antioxidative systems such as the ascorbate-glutathione cycle and is also necessary for the generation of superoxide radicals by plant NADPH oxidases and for the generation of nitric oxide (NO) by L-arginine-dependent nitric oxide synthase. This coenzyme is principally re-generated by a group of NADP-dehydrogenases enzymes including glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH), both belonging to the pentose phosphate pathway, the NADP-malic enzyme (NADP-ME), and NADP-isocitrate dehydrogenase (NADP-ICDH). In this study, current perspectives on these enzymes in higher plants under different stress situations are reviewed and it is also pointed out that this group of NADPH-generating dehydrogenases is a key element in supporting the mechanism of response to nitro-oxidative stress situations.

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Section: Function Of Nadp-dehydrogenases Under Environmental Stress Csupporting

confidence: 57%

NADPH-generating dehydrogenases: their role in the mechanism of protection against nitro-oxidative stress induced by adverse environmental conditions

Corpas

Barroso

2014

Front. Environ. Sci.

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“…It is well known that overexpression of ICE and ICE-like genes can intensely induced the expression of CBF3 in plants [41]. Here, CBF3, KIN1, COR15, and COR47A were strongly expressed not only in the transgenic lines of EcaICE1, similar to our precious studies [42], but also in those of its mutants, than the WT and ice1 mutant after cold treatment (Fig. 6), showing that the site-direct mutagenesis of EcaICE1 did not affect its functions in Arabidopsis.…”

Section: Discussionsupporting

confidence: 89%

Characterization of the Key Region and Key Phosphorylation Sites of EcaICE1 for its Molecular Interaction with EcaHOS1 Protein in Eucalyptus camaldulensis

Cheng

Zhang

et al. 2020

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Background ICE1 (inducer of CBF expression 1), a MYC-like bHLH transcriptional activator, plays an important role in plant under cold stress via regulating transcriptional expression of downstream cold-responsive genes. Ubiquitination-proteasome pathway mediated by high expression of osmotically responsive gene1 (HOS1) can effectively induce the degradation of ICE1 and decrease the expression of expression of CBFs and their downstream genes under cold stress response in Arabidopsis , but the knowledge about ubiquitination regulation of ICE1 by HOS1 is still unknown in woody plants.Results The complete EcaICE1 gene and a new E3 ubiquitin ligase gene EcaHOS1 were amplified from the tissue culture seedlings of Eucalyptus camaldulensis . Yeast two-hybrid (Y2H) and BiFC assay results showed that EcaICE1 can interact with EcaHOS1 protein in the nucleus, and further Y2H assay demonstrated that the 126-185 amino acid region at the N-terminus of EcaICE1 protein was indispensable for its interaction with EcaHOS1 protein. Moreover, we found that the amino acids at positions 143, 145, 158 and 184 within the key interaction region were the potential phosphorylation sites of EcaICE1 based on bioinformatics analysis, and that only the substitution of Serine (Ser) 158 by Alanine (Ala) blocked the protein-protein interactions between EcaICE1 and EcaHOS1 by Y2H and β-galactosidase assays using site-direct mutagenesis. Overexpression of EcaICE1 and its mutations in Arabidopsis could significantly increase POD and SOD activities with a reduction for MDA content and up-regulate four cold-responsive genes ( CBF3 , KIN1 , COR15 and COR47A ) in the transgenic lines.Conclusion We first reported that EcaICE1 could interact with EcaHOS1 protein in Eucalyptus , and identified Ser 158 of EcaICE1 as the key phosphorylation site for its interaction with EcaHOS1 protein.

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“…In addition, the functionality of the plastids might be increased by the glucose‐6‐phosphate dehydrogenase (G6PDH) gene, CUFF.27096. Under cold stress conditions, NADPH, which is necessary for carbon fixation and fatty acid synthesis, is provided by G6PDH, and tobacco lines overexpressing G6PDH were reported to show increased tolerance to cold temperature (Lin et al ., ; Yang et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Apple fruit superficial scald resistance mediated by ethylene inhibition is associated with diverse metabolic processes

et al. 2017

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Fruits stored at low temperature can exhibit different types of chilling injury. In apple, one of the most serious physiological disorders is superficial scald, which is characterized by discoloration and brown necrotic patches on the fruit exocarp. Although this phenomenon is widely ascribed to the oxidation of α-farnesene, its physiology is not yet fully understood. To elucidate the mechanism of superficial scald development and possible means of prevention, we performed an integrated metabolite screen, including an analysis of volatiles, phenols and lipids, together with a large-scale transcriptome study. We also determined that prevention of superficial scald, through the use of an ethylene action inhibitor, is associated with the triggering of cold acclimation-related processes. Specifically, the inhibition of ethylene perception stimulated the production of antioxidant compounds to scavenge reactive oxygen species, the synthesis of fatty acids to stabilize plastid and vacuole membranes against cold temperature, and the accumulation of the sorbitol, which can act as a cryoprotectant. The pattern of sorbitol accumulation was consistent with the expression profile of a sorbitol 6-phosphate dehydrogenase, MdS6PDH, the overexpression of which in transgenic Arabidopsis thaliana plants confirmed its involvement in the cold acclimation and freezing tolerance.

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Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants

Cited by 33 publications

References 10 publications

NADPH-generating dehydrogenases: their role in the mechanism of protection against nitro-oxidative stress induced by adverse environmental conditions

NADPH-generating dehydrogenases: their role in the mechanism of protection against nitro-oxidative stress induced by adverse environmental conditions

Characterization of the Key Region and Key Phosphorylation Sites of EcaICE1 for its Molecular Interaction with EcaHOS1 Protein in Eucalyptus camaldulensis

Apple fruit superficial scald resistance mediated by ethylene inhibition is associated with diverse metabolic processes

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