Functional analysis of the cell cycle protein E gene (ccne) in ovarian development of the white ridgetail prawn, Exopalaemon carinicauda

Zhang, Simian; Pang, Zhiyu; Gao, Jiayi; Dai, Qin; Liu, Xue; Shen, Yuxin; Baloch, Wazir Ali; Noonari, Sumera; Wang, Panpan; Gao, Huan

doi:10.1016/j.aqrep.2023.101716

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Such high levels of CCNE mRNA in gonads may be preserved via sex hormones [30]. CCNE is expressed at the highest level in stage III during ovarian development and induced yolk protein vitellogenin expression [31], but is not involved in the initiation of oocyte maturation [32]. In addition, cyclin E degradation results in reduced sperm motility duration in the frozen-thawed spermatozoa of sea bass [33], suggesting that cyclin E may play important roles in stem cell maintenance and gamete survival.…”

Section: Discussionmentioning

confidence: 99%

The Identification of a Cell Cycle Regulation Gene Cyclin E from Hong Kong Oysters (Crassostrea hongkongensis) and Its Protein Expression in Response to Salinity Stress

Qiu,

Wang,

Yan

et al. 2024

Fishes

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Hong Kong oysters (Crassostrea hongkongensis) are an important marine bivalve with nutritional and commercial value. The expanded off-bottom farming scale in recent years makes the oysters more susceptible to exposure to abiotic stresses, such as salinity stress, an important environmental factor that has been proven to have significant effects on oyster growth and development. However, the molecular mechanism is still unclear. Cyclin E is an important protein in the process of cell cycle regulation that is indispensable for propelling G1/S phase transition in a dose-dependent manner. In order to investigate whether the salinity stress affects cyclin E expression in oysters, the cDNA sequence of C. hongkongensis cyclin E (Ch-CCNE) was isolated from a gill cDNA library, and the 2.8 kbp length cDNA fragment contained a complete open reading frame (ORF) encoding 440 amino acid residues. Ch-CCNE mRNA was highly expressed in the gonad and low in the adductor mussel, mantle, gill, labial palp, and digestive gland. The recombinant CCNE protein was expressed and purified in a pET32a(+)-CCNE/Escherichia coli BL21(DE3) system via IPTG induction and was used for generating mice anti-Ch-CCNE antiserums. Western blot analysis showed that the CCNE protein in the gill was maintained at low expression levels under either hypo- (5 ppt) or hyper- (35 ppt) salinity, and could be produced at high levels under appropriate salinity during a 10-day exposure period. The immuno-localization indicated that the Ch-CCNE protein was distributed in the nucleus. These results suggested that either hypo- or hyper-salinity stress could inhibit the CCNE expression of Hong Kong oysters and their negative impact on cell division and proliferation.

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Section: Discussionmentioning

confidence: 99%

The Identification of a Cell Cycle Regulation Gene Cyclin E from Hong Kong Oysters (Crassostrea hongkongensis) and Its Protein Expression in Response to Salinity Stress

Qiu,

Wang,

Yan

et al. 2024

Fishes

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Comprehensive review on evolution, progression, design, and exploration of electric bicycle

et al. 2024

The Archives of Automotive Engineering – Archiwum Motoryzacji

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Many countries are moving to electric vehicles for daily transportation due to the scarcity of fossil fuels and the pollution emissions from internal combustion engines. The most promising technique to manage environmental pollution and energy security is the employment of electric vehicles. The electric bicycle is gaining an abundance of attention due to the various advantages of electric vehicles. Recently, people started using electric bicycles on a regular basis, and it is a suitable option for shorter daily rides. The present study was conducted using the Scopus database, which included an analysis of all papers pertaining to the content of electric bicycles up until the year 2023.The literature review delves into the profound impact and numerous benefits of electric bicycles, highlighting their potential for revolutionizing transportation. This exploration is informed by advancements in design and construction, offering insights into optimal electric bicycle development. One promising avenue for sustainability involves integrating solar technology into these vehicles, reducing reliance on conventional energy sources, and addressing environmental concerns. Discussions encompassed diverse aspects, including operation principles, throttle variations, commutation systems, self-charging mechanisms, health advantages, and solar-powered electric bicycles. To address this, the goal is to create universally acceptable electric bicycles to broaden their appeal and dispel misconceptions. In order to provide scientists and researchers with beneficial information to further optimize electric bicycles, the research fields pertaining to electric bicycles are reviewed in accordance with previous studies.

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Functional analysis of the cell cycle protein E gene (ccne) in ovarian development of the white ridgetail prawn, Exopalaemon carinicauda

Cited by 2 publications

References 12 publications

The Identification of a Cell Cycle Regulation Gene Cyclin E from Hong Kong Oysters (Crassostrea hongkongensis) and Its Protein Expression in Response to Salinity Stress

The Identification of a Cell Cycle Regulation Gene Cyclin E from Hong Kong Oysters (Crassostrea hongkongensis) and Its Protein Expression in Response to Salinity Stress

Comprehensive review on evolution, progression, design, and exploration of electric bicycle

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