Functional analysis of transgenic rice (Oryza sativaL.) transformed with anArabidopsis thalianaferric reductase (AtFR02)

Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil

“…The Arabidopsis Fe 3ϩ chelate-reductase gene with its own promoter was introduced into rice plants, but the transgene was not detected at the mRNA level (20). We therefore considered it crucial to use a preferred promoter for functional refre1.…”

Identification of up-regulated genes in flag leaves during rice grain filling and characterization of OsNAC5, a new ABA-dependent transcription factor

“…However, the lack of knowledge about how nutrients are moved into or out of vascular tissues, translocated to vegetative tissues and loaded into seeds is one of the barriers to biofortiWcation of seeds (Colangelo and Guerinot 2006;Kramer et al 2007). Initial seed biofortiWcation eVorts for Fe and Zn in rice have focused on increasing the iron storage protein ferritin (Goto et al 1999;Vasconcelos et al 2003) or root ferric reductase activity (Vasconcelos et al 2004). The transgenic plants showed an overaccumulation of minerals in leaves, but only a small increase in seeds.…”

Identification of new QTLs for seed mineral, cysteine, and methionine concentrations in soybean [Glycine max (L.) Merr.]

“…Initial efforts in biofortification of seeds have focused on overexpression of single genes that affected mineral uptake, transport or storage (Goto et al 1999;Ramesh et al 2004;Vasconcelos et al 2003Vasconcelos et al , 2004Vasconcelos et al , 2006. Analysis of these plants suggested that seed accumulation of minerals is tightly regulated, and overexpression of a single gene was typically not sufficient to increase the accumulation of minerals in seeds (Goto et al 1999;Ishimaru et al 2007Ishimaru et al , 2010Qu et al 2005;Vasconcelos et al 2003Vasconcelos et al , 2004Vasconcelos et al , 2006. It is likely that multiple genes at different steps of translocation or biosynthetic pathways will need to be simultaneously overexpressed to increase seed mineral concentrations (Waters and Sankaran 2011).…”