Functional and biochemical characterizations of avian T lymphocyte antigens identified by monoclonal antibodies

Abstract

Seven monoclonal antibodies (mAb) were used to characterize antigens present on chicken T lymphocytes and on natural killer cells by flow cytometry, radioimmunoprecipitation and by effects on cell-mediated cytotoxicity and mitogen-induced proliferation. mAb CTLA8 and 5 stained 73% of thymus, 44% of spleen and 51% of peripheral blood lymphocytes (PBL), respectively, and immunoprecipitated 65- and 45-kDa proteins from detergent extracts of 125I surface-labeled thymocytes. Pretreatment of splenic lymphocytes with mAb CTLA5 and 8 in the presence of rabbit complement (C) eliminated the concanavalin A (Con A)-induced T cell proliferative responses. mAb CTLA3, 4 and 9 stained 43% of thymus, 36% of spleen and 18% of PBL, and immunoprecipitated 33-35-kDa proteins. Pretreatment of spleen cells with mAb 4 or 9 plus C reduced, but did not eliminate, the Con A-induced proliferative response and significantly reduced both major histocompatibility complex (MHC)-restricted and non-MHC-restricted cellular cytotoxicity. mAb CTLA1 and 6 stained 58% of thymus, 13% of spleen and 19% of PBL. mAb CTLA1 and 6 immunoprecipitated a 65-kDa protein. mAb CTLA1 and 6 had no effect on the Con A-induced blastogenesis and CTLA6 caused no decrease in virus-specific cytotoxic T lymphocyte and natural killer activity. These results indicate that (a) mAb CTLA5 and 8 identify antigens on mature T lymphocytes that are similar in tissue distribution, molecular mass and function to the mammalian CD5 antigen; (b) mAb CTLA3, 4 and 9 detect the avian homologue of CD8 antigen; and (c) mAb CTLA1 and 6 identify the avian homologue of CD4 antigen.

The pp38 Gene of Marek's Disease Virus (MDV) Is Necessary for Cytolytic Infection of B Cells and Maintenance of the Transformed State but Not for Cytolytic Infection of the Feather Follicle Epithelium and Horizontal Spread of MDV

“…The same letter placed next to numbers within a replicate means that the differences were not significant. (6,11,12; data not shown). In the lymphoproliferative lesions induced by rMd5⌬pp38 that could be detected only by microscopic examination, many cells had homogeneously condensed chromatin, and numerous apoptotic bodies could be detected (Fig.…”

JMV‐1 stimulation of avian natural killer cell activity

“…Avian NK cells have been described as non-T, non-B, non-macrophage cells, bearing Fc receptors, by Lam & Linna, (1980). Lillehoj et al (1988), further characterized avian NK cells by using monoclonal antibodies and showed that their molecular and functional characteristics are very similar to those found for human and mouse NK cells.…”

Participation of a chicken thymocyte antigen in intrathymic differentiation of T cellsin vivo

“…In chickens, the sequence of events in the thymic microenvironment during T cell differentiation is poorly understood. However, it has been shown that T cells express molecules homologous to mammalian CD3 (Chen et al, 1986), TCR Sowder et al, 1988;Char et al, 1990), CD4 and CD8 (Chan et al, 1988;Lillehoj et al, 1988;Kondo et al, 1990). Moreover, several antigens specifically expressed on thymocytes have also been reported, and ontogenies and biochemical features of these membrane antigens have been analysed (Pink & Rijnbeek, 1983;Chén et al, 1984;Kondo et al, 1988).…”