Functional and Clinical Proteomic Exploration of Pancreatic Cancer

Huang, Peiwu; Gao, Weina; Fu, Changying; Tian, Ruijun

doi:10.1016/j.mcpro.2023.100575

Cited by 9 publications

(3 citation statements)

References 95 publications

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“…Our results showed many DEPs related to the immune system and correlated processes. Recently, Huang and collaborators [ 45 ] have compiled the recent advances in PDAC proteomic studies and explored how they can contribute to clinical development. They showed results using plasma, tissue, and exosomes proposing a panel of proteins that together have the potential as diagnosis and prognosis biomarkers.…”

Section: Discussionmentioning

confidence: 99%

Screening of Exosome-Derived Proteins and Their Potential as Biomarkers in Diagnostic and Prognostic for Pancreatic Cancer

Marin

Batista

Azevedo

et al. 2023

IJMS

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In the oncological area, pancreatic cancer is one of the most lethal diseases, with 5-year survival rising just 10% in high-development countries. This disease is genetically characterized by KRAS as a driven mutation followed by SMAD4, CDKN2, and TP53-associated mutations. In clinical aspects, pancreatic cancer presents unspecific clinical symptoms with the absence of screening and early plasmatic biomarker, being that CA19-9 is the unique plasmatic biomarker having specificity and sensitivity limitations. We analyzed the plasmatic exosome proteomic profile of 23 patients with pancreatic cancer and 10 healthy controls by using Nanoscale liquid chromatography coupled to tandem mass spectrometry (NanoLC-MS/MS). The pancreatic cancer patients were subdivided into IPMN and PDAC. Our findings show 33, 34, and 7 differentially expressed proteins when comparing the IPMN vs. control, PDAC-No treatment vs. control, and PDAC-No treatment vs. IPMN groups, highlighting proteins of the complement system and coagulation, such as C3, APOB, and SERPINA. Additionally, PDAC with no treatment showed 11 differentially expressed proteins when compared to Folfirinox neoadjuvant therapy or Gemcitabine adjuvant therapy. So here, we found plasmatic exosome-derived differentially expressed proteins among cancer patients (IPMN, PDAC) when comparing with healthy controls, which could represent alternative biomarkers for diagnostic and prognostic evaluation, supporting further scientific and clinical studies on pancreatic cancer.

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Section: Discussionmentioning

confidence: 99%

Screening of Exosome-Derived Proteins and Their Potential as Biomarkers in Diagnostic and Prognostic for Pancreatic Cancer

Marin

Batista

Azevedo

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…This special issue of Molecular and Cellular Proteomics presents a wide range of primary research, reviews, and perspectives pertaining to clinical proteomics. The expanding scope of proteomics applications is reflected in the spectrum of disease foci represented, including neurodegenerative diseases ( 1 , 2 , 3 , 4 ), infectious disease ( 5 , 6 ), endocrinology ( 7 , 8 ), cardiovascular disease ( 9 ), inflammatory disease ( 10 ), kidney disease ( 11 ), and cancer ( 12 , 13 , 14 , 15 ), as well as in the burgeoning areas of health monitoring and exercise ( 16 , 17 ).…”

mentioning

confidence: 99%

“…MS-based proteomics remains especially important to deepening our understanding of signaling biology and the effects of disease and drug perturbations on posttranslational modifications such as phosphorylation, ubiquitylation, acetylation, sumoylation, and glycosylation. Improved methods now enable these and many other PTMs of importance ( 12 ) to be analyzed at deep scale, individually or as part of serial enrichment and analysis strategies yielding global proteome and numerous PTMs from exactly the same sample, thereby facilitating multi-omic integration while sparing precious patient material. Such comprehensive molecular portraits are much more likely to inform our understanding of disease than genomic characterization alone.…”

mentioning

confidence: 99%