Functional and Comparative Analysis of Two Subtypes of Cofilin Family on Cattle Myoblasts Differentiation

Abstract

Agricultural meat composition and quality are not independent of the effects of skeletal muscle growth and development in animals. Cofilin is distributed extensively in muscle and non-muscle cells, and its function is tightly regulated in the cell. Cofilin has two variants in mammals, cofilin-1 (CFL1, non-muscle type) and cofilin-2 (CFL2, muscle type), and has a dual function on skeletal muscle fibers. Our study examined the expression pattern of CFL1 and CFL2 in different fetal bovine, calf, and adult cattle tissues. The content of the CFL2 gene increased significantly with the increase in cattle age in muscle tissues; CFL1 showed the opposite trend. In muscle tissues, DNA methylation levels of CFL1 and CFL2 were high in fetal bovine, and the mRNA level of CFL2 was significantly lower compared to CFL1. However, DNA methylation levels of CFL2 were lower than CFL1, and the mRNA level of CFL2 was remarkably higher compared to CFL1 in adult cattle. Overexpression of CFL1 or knockdown CFL2 reduced the expression levels of muscle differentiation markers, i.e., MYOD, MYOG, and MYH3. Overexpression of CFL2 or knockdown CFL1 stimulated the expression of these marker genes. Therefore, CFL2 may be superior to CFL1 as a candidate gene for subsequent research on cattle genetics and breeding.

Identification cloning and functional analysis of novel natural antisense lncRNA CFL1-AS1 in cattle

“…NAT transcript sequences partially or completely overlap and complement mRNA, promoter or regulatory regions of coding genes and participate in gene expression regulation at the transcriptional and posttranscriptional levels [ 40 ]. CFL1 and CFL2 play an important role in the growth and differentiation of skeletal muscle [ 18 ]. We predicted that the sequence of CFL1-AS1 can complement the partial sequences of CFL1 and CFL2, suggesting that CFL1-AS1 May regulate the development and differentiation of skeletal muscle by affecting the expression of CFL1 and CFL2.…”

“…Both the CFL2 gene and CFL1-AS1 parent gene CFL1 are closely related to muscle growth and development and play an important role in normal muscle function and regeneration. Previous studies have shown that the CFL1 and CFL2 genes are specifically expressed in different growth and development stages of bovine muscle and show opposite expression patterns during muscle cell differentiation [ 17 , 18 ]. RNAhybrid analysis identified a miR-125b binding site on CFL1-AS1, and studies have shown that miR-125b can regulate the proliferation and differentiation of myoblasts [ 19 ].…”

Genetics and Production of Safe, High-Quality Milk by Dairy Cattle

“…In future production practices, it is important to pay special attention to mutations associated with abnormalities in dairy-cattle populations, such as defects in bovine leukocyte adhesion, complex vertebral malformations, and short spinal or short spinal lethal syndrome, as representative examples of inherited diseases [8]. Meanwhile, two variants of cofilin, cofilin-1 (CFL1, non-muscular type) and cofilin-2 (CFL2, muscular type), have been found in mammals, with a dual function on skeletal muscle fibers [9]. The acute phase response (APR) is a non-specific systemic response in which acute phase protein (APP) production changes.…”