Functional and DNA Sequence Divergence of the CYP71 Gene Family in Higher Plants

Christoffersen, Rolf E.; Percival, Frank W.; Bozak, Kristin R.

doi:10.1515/dmdi.1995.12.3-4.207

Cited by 13 publications

(5 citation statements)

References 24 publications

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“…The CYP81A6 sequence also showed similarity to the other hydroxylase proteins (Fig. 2E) such as CYP71A1 (Christoffersen et al 1995), CYP75B1/B2 (Brugliera et al 1999;Rupasinghe et al 2003), CYP76A1/B6 Collu et al 2001), CYP82A1/B1 proteins. According to the nomenclature for the P450 superfamily (http://drnelson.utmem.edu/CytochromeP450.…”

Section: Fine Mapping Of Belmentioning

confidence: 71%

Map-based cloning of a novel rice cytochrome P450 gene CYP81A6 that confers resistance to two different classes of herbicides

Pan

Xianyin

Liu³

et al. 2006

Plant Mol Biol

128

119

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Development of hybrid rice has greatly contributed to increased yields during the past three decades. Two bentazon-lethal mutants 8077S and Norin8m are being utilized in developing new hybrid rice systems. When the male sterile lines are developed in such a mutant background, the problem of F1 seed contamination by self-seeds from the sterile lines can be solved by spraying bentazon at seedling stage. We first determined the sensitivity of the mutant plants to bentazon. Both mutants showed symptoms to bentazon starting from 100 mg/l, which was about 60-fold, lower than the sensitivity threshold of their wild-type controls. In addition, both mutants were sensitive to sulfonylurea-type herbicides. The locus for the mutant phenotype is bel for 8077S and bsl for Norin8m. Tests showed that the two loci are allelic to each other. The two genes were cloned by map-based cloning. Interestingly, both mutant alleles had a single-base deletion, which was confirmed by PCR-RFLP. The two loci are renamed bel ( a ) (for bel) and bel ( b ) (for bsl). The wild-type Bel gene encodes a novel cytochrome P450 monooxgenase, named CYP81A6. Analysis of the mutant protein sequence also revealed the reason for bel ( a ) being slightly tolerant than bel ( b ). Introduction of the wild-type Bel gene rescued the bentazon- and sulfonylurea-sensitive phenotype of bel ( a ) mutant. On the other hand, expression of antisense Bel in W6154S induced a mutant phenotype. Based on these results we conclude that the novel cytochrome P450 monooxygenase CYP81A6 encoded by Bel confers resistance to two different classes of herbicides.

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Section: Fine Mapping Of Belmentioning

confidence: 71%

Map-based cloning of a novel rice cytochrome P450 gene CYP81A6 that confers resistance to two different classes of herbicides

Pan

Xianyin

Liu³

et al. 2006

Plant Mol Biol

128

119

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“…This homology is of special interest since CYP82A1 is a wound-inducible P450 from pea, a legume with a wound-or defense-related secondary metabolism similar to soybean. Three cDNAs, CYP71A9, CYP71D8 and CYP71D9, belong to the CYP71 family, which seems to play a variety of functional roles, among them terpenoid metabolism (Christoersen et al 1995). Because of this divergence, clues as to its biochemical functions are lacking.…”

Section: Isolation Of Full-length Cdnasmentioning

confidence: 99%

Identification of elicitor-induced cytochrome P450s of soybean (Glycine max L.) using differential display of mRNA

Ebel

1998

Mol Gen Genet

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Elicitor-inducible glyceollin biosynthesis in soybean depends on five presumably transcriptionally regulated cytochrome P450-dependent enzymes (P450s). In order to isolate corresponding cDNA clones, we devised a novel polymerase chain reaction (PCR)-based approach targeting P450s that are transcriptionally activated under glyceollin-inducing conditions. The differential display of mRNA (DD-RT-PCR) technique was performed with upstream primers based on the conserved heme-binding region of P450s, and ten different 3'-terminal partial P450 sequences were isolated. They were subsequently used to isolate nine different full-length cDNA clones from a cDNA library. As shown by Northern blot analysis, eight of the clones represented P450s, which were activated under glyceollin-inducing conditions similar to two enzymes of the glyceollin biosynthesis pathway, CHS and IFR. Therefore, these eight clones are candidate cDNAs for the glyceollin-related P450s. Functional expression in yeast identified one cDNA clone coding for cinnamate 4-hydroxylase. Thus, at least one of the isolated clones definitively encodes a P450 of the glyceollin pathway. Consequently, this approach offers a straightforward alternative to classical P450 isolation strategies via protein purification and should prove especially useful for isolating P450s that are expressed at a low level.

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“…As each CYP catalyses a different reaction, it is unlikely that all CYPs play a role in the diterpene glycoside synthesis. Four CYP families, from which seven members were found in the QTL region as defined by Maharijaya et al ( 2015 ), were previously suggested to play a role in terpenoid metabolism (Christoffersen Rolf et al 1995 ; Schopfer and Ebel 1998 ; Ro et al 2005 ; Ohnishi et al 2006 ; Thornton et al 2010 ; Höfer et al 2013 ). The CYP89 and CYP94 families, from which eight members were found in the QTL region, do not seem to play a role in the terpenoid pathway (Kahn et al 2001 ; Christ et al 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Fine mapping of a thrips resistance QTL in Capsicum and the role of diterpene glycosides in the underlying mechanism

et al. 2021

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Key message A major thrips resistance QTL in Capsicum was fine-mapped to a region of 0.4 Mbp, and a multidisciplinary approach has been used to study putative underlying mechanisms. Abstract Resistance to thrips is an important trait for pepper growers. These insects can cause extensive damage to fruits, flowers and leaves on field and greenhouse grown plants worldwide. Two independent studies in Capsicum identified diterpene glycosides as metabolites that are correlated with thrips resistance. In this study, we fine-mapped a previously defined thrips resistance QTL on chromosome 6, to a region of 0.4 Mbp harbouring 15 genes. Two of these 15 candidate genes showed differences in gene expression upon thrips induction, when comparing plants carrying the resistance allele in homozygous state to plants with the susceptibility allele in homozygous state for the QTL region. Three genes, including the two genes that showed difference in gene expression, contained a SNP that was predicted to lead to changes in protein structure. Therefore, these three genes, i.e. an acid phosphatase 1 (APS1), an organic cation/carnitine transporter 7 (OCT7) and an uncharacterized locus LOC107874801, are the most likely candidates for playing a role in thrips resistance and are a first step in elucidating the genetic basis of thrips resistance in Capsicum. In addition, we show that the diterpene glycoside profiles did not differ between plants with the resistance and susceptibility allele for the chromosome 6 QTL, suggesting that these compounds do not play a role in the resistance conferred by the genes located in the major thrips resistance QTL studied.

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Functional and DNA Sequence Divergence of the CYP71 Gene Family in Higher Plants

Cited by 13 publications

References 24 publications

Map-based cloning of a novel rice cytochrome P450 gene CYP81A6 that confers resistance to two different classes of herbicides

Map-based cloning of a novel rice cytochrome P450 gene CYP81A6 that confers resistance to two different classes of herbicides

Identification of elicitor-induced cytochrome P450s of soybean (Glycine max L.) using differential display of mRNA

Fine mapping of a thrips resistance QTL in Capsicum and the role of diterpene glycosides in the underlying mechanism

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