Functional and hepatic metabolite changes in aquatic turtle hatchlings exposed to the anti-androgenic fungicide vinclozolin

Lu, Hong-Liang; Kang, Chun-Quan; Meng, Qin-Yuan; Hu, Jian-Rao; Melvin, Steven D.

doi:10.1016/j.ecoenv.2022.113220

Cited by 10 publications

(4 citation statements)

References 61 publications

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“…In addition to these restrictions inside the United States, as of 2006, the use of this pesticide was restricted in numerous nations, including Denmark, Finland, Norway, and Sweden. To assess its risks and hazards to the environment and animals, a number of tests and regulations have been applied [ 3 , 4 ]. Vinclozolin is created as extruded or dried flowable granules.…”

Section: Introductionmentioning

confidence: 99%

Chronic Exposure to Endocrine Disruptor Vinclozolin Leads to Lung Damage via Nrf2–Nf-kb Pathway Alterations

D’Amico

Paola

Impellizzeri

et al. 2022

IJMS

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Endocrine-disrupting substances (EDS) are common and pervasive in our environment and pose a serious risk to both human and animal health. Endocrine-disrupting compounds (EDCs) have been associated with a variety of detrimental human health effects, including respiratory issues, as a result of their ability to disrupt cell physiology. Vinclozolin ((RS)-3-(3,5-Dichlorophenyl)-5-methyl-5-vinyloxazolidine-2,4-dione) is a common dicarboximide fungicide used to treat plant diseases. Several studies have analyzed the effects of vinclozolin exposure on the reproductive system, but less is known about its effect on other organs such as the lung. Mice were exposed for 28 days to orally administered vinclozolin at a dose of 100 mg/kg. Vinclozolin exposure induced histological alterations and collagen depositions in the lung. Additionally, vinclozolin induced inflammation and oxidative stress that led to lung apoptosis. Our study demonstrates for the first time that the toxicological effects of vinclozolin are not limited to the reproductive system but also involve other organs such as the lung.

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Section: Introductionmentioning

confidence: 99%

Chronic Exposure to Endocrine Disruptor Vinclozolin Leads to Lung Damage via Nrf2–Nf-kb Pathway Alterations

D’Amico

Paola

Impellizzeri

et al. 2022

IJMS

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“…Some amino acids can participate in energy metabolism through specific metabolic pathways, such as being converted into the TCA cycle intermediates [ 48 ]. For example, leucine may enter the TCA cycle by being converted to citrate [ 7 , 29 ]. Accordingly, reductions in relevant amino acids in this study would potentially exert adverse impacts in these metabolic pathways and thus disturb energy metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…Hepatic metabolomic data processing was performed as previously described [ 29 ]. Multivariate unsupervised principal component analysis (PCA) and supervised partial least squares–discriminant analysis (PLS-DA) was performed to test among-group differences in hepatic metabolites.…”

Section: Methodsmentioning

confidence: 99%

Gut Microbial Composition and Liver Metabolite Changes Induced by Ammonia Stress in Juveniles of an Invasive Freshwater Turtle

Meng

Wang

et al. 2022

Biology

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As the most common pollutant in aquaculture systems, the toxic effects of ammonia have been extensively explored in cultured fish, molluscs, and crustaceans, but have rarely been considered in turtle species. In this study, juveniles of the invasive turtle, Trachemys scripta elegans, were exposed to different ammonia levels (0, 0.3, 3.0, and 20.0 mg/L) for 30 days to evaluate the physiological, gut microbiomic, and liver metabolomic responses to ammonia in this turtle species. Except for a relatively low growth rate of turtles exposed to the highest concentration, ammonia exposure had no significant impact on the locomotor ability and gut microbial diversity of turtles. However, the composition of the microbial community could be altered, with some pathogenic bacteria being increased in ammonia-exposed turtles, which might indicate the change in their health status. Furthermore, hepatic metabolite profiles via liquid chromatography–mass spectrometry revealed extensive metabolic perturbations, despite being primarily involved in amino acid biosynthesis and metabolism. Overall, our results show that ammonia exposure causes gut dysbacteriosis and disturbs various metabolic pathways in aquatic turtle species. Considering discrepant defense mechanisms, the toxic impacts of ammonia at environmentally relevant concentrations on physiological performance might be less pronounced in turtles compared with fish and other invertebrates.

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“…The long-term damage caused by VCZ can be even greater, such as damage at behavioral level, affiliative behavior, or motor inhibition in mammals, but also in different class of animals like birds. In addition, renal damage from chronic VCZ exposure has also been seen in fish and in the liver metabolic profile of aquatic organisms [ 11 , 12 , 22 , 23 , 24 ]. Studies on the toxic action of VCZ have shown the exposure to this pesticide to be a risk factor in different time windows, not only in the long term.…”

Section: Introductionmentioning

confidence: 99%

Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial Damage

Peritore

Franco

Molinari

et al. 2023

Toxics

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(1) Background: Vinclozolin is a popular fungicide used in fruit, ornamental plants, and vegetable crops. It has recently been seen that prolonged exposure to VZN can cause human or animal health damage to various organs, but little is known to date about its cardiovascular effects. In this study, we addressed the chronic effects of VZN on the myocardium and the enzymes involved in the cardiovascular function. (2) Methods: The animals were divided into four groups: group 1 served as the control, group 2 received 1 mg/kg of VZN by gavage, group 3 received 30 mg/kg of VZN by gavage, and group 4 received 100 mg/kg of VZN by gavage, for 30 days. (3) Results: Results showed that 100 mg/kg VZN markedly increased the plasma concentration of cardiac markers (CK-MB, cTnT, ANP, BNP). Moreover, compared to the control group, VZN treatment decreased the activity of SOD, CAT, and GPx, and downregulated the mRNA expression levels of Nrf2. Furthermore, collagen deposition was amplified owing to 100 mg/kg VZN cardiotoxicity. This harmful effect was confirmed by a histological study using hematoxylin and eosin (H&E) and Masson’s trichrome staining. (4) Conclusion: Overall, our results proved the cardiotoxicity caused by chronic exposure to VZN.

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Functional and hepatic metabolite changes in aquatic turtle hatchlings exposed to the anti-androgenic fungicide vinclozolin

Cited by 10 publications

References 61 publications

Chronic Exposure to Endocrine Disruptor Vinclozolin Leads to Lung Damage via Nrf2–Nf-kb Pathway Alterations

Chronic Exposure to Endocrine Disruptor Vinclozolin Leads to Lung Damage via Nrf2–Nf-kb Pathway Alterations

Gut Microbial Composition and Liver Metabolite Changes Induced by Ammonia Stress in Juveniles of an Invasive Freshwater Turtle

Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial Damage

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