Functional and Metabolic Features of Blood Phagocytes at Different Forms of Tubercular Inflammatory Process of Lungs

Викторовна, Бердюгина Ольга; Ершова, А. В.

doi:10.15789/1563-0625-2016-1-21-32

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…3), так же как и общее количество лимфоцитов, был снижен почти в 2 раза у больных с туберкулемами легких, выявленными в среднем за 0,5 года до исследования (в сравнении с контрольной группой, p k-w < 0,016). Сходные изменения уже наблюдались нами ранее [1], однако у больных с туберкулемами легких, выявленными в среднем за 9,5 лет до исследования количество NK-клеток не отличалось от группы здоровых людей, что представляет новые данные этой работы.…”

Section: интегриновые рецепторы при туберкулемах легкихunclassified

Is there a dependence between expression of integrin receptors by peripheral blood immune cells and duration of tuberculous granuloma existence in the patients?

Бердюгина

2020

Med. immunol.

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Over recent years, the number of patients with tuberculosis has not decreased in the country and in worldwide. This is due to high resistance of the pathogen and changing mechanisms of bacterial perception by the human immune system thus requiring closer examination of the issue. Cell fusion during the formation of pulmonary tuberculous granuloma involves a large number of adhesive events. Importance of α1β1 integrin has been shown for the granuloma integrity during the chronic phase of infection. It has been proven that pulmonary tuberculous granuloma should be monitored, including with the detection of cells expressing CD11c, since they support the continuous priming of T cells at different stages of infection. The aim of this study was to answer the question, if there is a different expression of integrin receptors by immune cells from the patient’s peripheral blood at different stages of the existence of pulmonary tuberculous granuloma? The study involved 38 people: the first group (control) consisted of 15 practically healthy people; a second group included 11 subjects with pulmonary tuberculous granuloma; the condition was first diagnosed 2 to 10 months before the present study. A third group consisted of 12 patients with pulmonary tuberculous granuloma, with primary diagnosis established 12 to 219 months before this study. All the participants underwent a general clinical blood tests using a 5 Diff Mythic 22 AL analyzer (Cormay, Poland). The adhesion markers CD11b, CD11c were detected with a Coulter Epicx XL instrument (Beckman Coulter, USA). The following peripheral blood cell populations were determined: CD14- CD13lowCD11b+, CD14- CD13lowCD11c+, CD14+CD11b+, CD14+CD11c+, CD45+CD3- CD16+CD56+, CD45+CD3- CD16+CD56+CD11b+. Statistical processing of the results was performed in the Windows 10 operating environment (Microsoft Corp., USA), using Statistica v. 12.5 software (StatSoft, USA). Kruskal–Wallis one-way analysis of variance (pk-w), with differences significant at p < 0.017, as well as the Wald–Wolfowitz test (pw-w) at a significance level of p < 0.05 were used as criteria for assessing differences between the compared groups. In addition, cluster and factor analysis were implemented. When studying the role of β2-integrins, we have found that they play an important role in maintaining the existence of pulmonary tuberculous granuloma. An increase in total number of granulocytes, and CD11b-expressing granulocytes, a decrease in the population of lymphocytes, NK cells and NK cells expressing CD11c proved to be distinctive in cases of pulmonary tuberculous granuloma detected 0.5 years before the study. Characteristic changes observed in the study of peripheral blood in the patients with pulmonary tuberculous granuloma detected 9.5 years before the study were as follows: an increase in the leukocyte population, total monocyte number, as well as CD11band CD11c-expressing monocytes.

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Section: интегриновые рецепторы при туберкулемах легкихunclassified

Is there a dependence between expression of integrin receptors by peripheral blood immune cells and duration of tuberculous granuloma existence in the patients?

Бердюгина

2020

Med. immunol.

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Methods of Estimation and the Role of Respiratory Burst in the Pathogenesis of Infectious and Inflammatory Diseases

Савченко

Кудрявцев

Борисов

2018

Russian Journal of Infection and Immunity

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According to the modern concepts the respiratory burst directly related to the processes of phagocytosis characterizes the functional activity of phagocytic cells. This review presents modern methods for assessing the respiratory burst state of phagocytes based on cytofluorometric and chemiluminescent analysis. The sequence and mechanisms of the reactions of reactive oxygen species (ROS) synthesis in the process of respiratory cell burst are presented in detail. The sequence of synthesis from ROS with low bactericidal activity to ROS with high bactericidal activity is characterized. The review describes in detail the most popular dyes for cytofluorometric analysis to assess the levels of ROS synthesis. Characteristics and examples of the use of such dyes as dihydroethidine, dichlorodihydrofluorescein and dihydrorhodamine 123 are given. The main stages and mechanisms of the chemiluminescence reaction are presented. The features of the use of the main indicators (luminol and lucigenin) of the chemiluminescence reaction are described. A mechanism for estimating the parameters of the chemiluminescence reaction characterizing the features of the state and kinetics of the respiratory burst of phagocytic cells is given. The separate section of the review is devoted to the role of a respiratory burst in phagocytic cells in various immunopathological states. Data on the pathogenetic significance of changes in the intensity and kinetics of respiratory burst of phagocytes in infectious, inflammatory and oncological diseases were presented. Examples of new methods for diagnosing and predicting the course of the immunopathological states characters are presented on the basis of an assessment of the respiratory burst of phagocytic cells. The literature data show that at present, in the diagnosis and evaluation of the nature of the diseases characters the state of a respiratory burst is evaluated in various types of cells of innate immunity: neutrophils, monocytes, etc. It is concluded that the evaluation of the respiratory burst of phagocytic cells can be characterized as the fundamental mechanisms of reacting cells of innate immunity to pathogenic and regulatory effects, so to develop new highly sensitive methods for diagnosing and predicting the development and outcome of various immunopathological conditions. The presented methods of flow cytometry and chemiluminescence analysis make it possible to determine both the integral state of the respiratory explosion, and the levels and kinetic parameters of the synthesis of individual ROS.

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Perspective Chapter: The Role of Interferon Gamma in Clinical Medicine

Rakityanskaya¹,

Ryabova²,

Kalashnikova³

et al. 2022

Basic and Clinical Aspects of Interferon Gamma

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Interferon gamma (IFN-γ) is one of the key factors of both innate and adaptive immune response that promotes differentiation of naive CD4+ cells into effector Th1 T cells producing the main mediators of cellular immunity against viral and intracellular bacterial infections, and specific cytotoxic immunity through the interaction of T cells with antigen-presenting cells and macrophage activation. The clinical importance of IFN-γ includes its medical use to treat and prevent various viral and bacterial infections. IFN-γ has a direct antiviral effect on infected cells, activates local infiltrating dendritic cells, macrophages and NK cells, modulates the differentiation and maturation of T and B cells, and enhances inflammation and antiviral functions. Immunoregulatory effect of IFN-γ plays one of the essential roles in the regulation of adaptive immune response in patients with tuberculosis infection and cancer. Producing IFN-γ by T cells increases the efficiency of infiltrated phagocytic cells, by stimulating NO and maintaining local host defense during tuberculosis infection. The direct antitumor effect of IFN-γ revealed in several experimental models has numerous mechanisms for the effect of development. IFN-γ has crucial potential for enhancing any antiviral, antimycobacterial, and specific antitumor therapies.

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Functional and Metabolic Features of Blood Phagocytes at Different Forms of Tubercular Inflammatory Process of Lungs

Cited by 3 publications

References 16 publications

Is there a dependence between expression of integrin receptors by peripheral blood immune cells and duration of tuberculous granuloma existence in the patients?

Is there a dependence between expression of integrin receptors by peripheral blood immune cells and duration of tuberculous granuloma existence in the patients?

Methods of Estimation and the Role of Respiratory Burst in the Pathogenesis of Infectious and Inflammatory Diseases

Perspective Chapter: The Role of Interferon Gamma in Clinical Medicine

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