Functional and Molecular Expression of AQP9 Channel and UT-A Transporter in Normal and Preeclamptic Human Placentas

Damiano, Alicia E.; Zotta, Elsa; Ibarra, Cristina

doi:10.1016/j.placenta.2005.11.014

Cited by 61 publications

(66 citation statements)

References 31 publications

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“…In fact, the human placenta can promote water passage from mother to fetus by either hydrostatic or osmotic mechanisms probably regulated by water channel proteins [1,2]. In vitro and animal studies [21,22] suggest that hypoxia and preeclampsia can influence amniotic fluid volume by interfering both with trans-placental water passage and trans-membranous water reabsorption. Venoconstriction caused by intervillous space/villus flow mismatching, as occur in placental underperfusion, can alter the transvillous pressure and affect the transplacental passage of liquids [19,23].…”

Section: Discussionmentioning

confidence: 99%

Placental lesions associated with oligohydramnios in fetal growth restricted (FGR) pregnancies

Spinillo¹,

Cesari²,

Bariselli³

et al. 2015

Placenta

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Section: Discussionmentioning

confidence: 99%

Placental lesions associated with oligohydramnios in fetal growth restricted (FGR) pregnancies

Spinillo¹,

Cesari²,

Bariselli³

et al. 2015

Placenta

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“…In placenta, AQP9 is highly expressed in syncytiotrophoblastic cells, where it is thought to play a role in fluid and urea transport (Damiano et al 2006). There has however been no evidence of AQP9 in the uterus during early pregnancy.…”

Section: Introductionmentioning

confidence: 99%

Aquaporins are upregulated in glandular epithelium at the time of implantation in the rat

Lindsay

Murphy

2007

J Mol Hist

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Regulation of luminal fluid is essential for blastocyst implantation. While it has been known for quite some time that there is a reduction in the amount of luminal fluid at the time of implantation, the mechanisms regulating this process are only just emerging. Previous studies have shown an upregulation of aquaporin (AQP) 5 channels in luminal epithelial cells at the time of implantation providing a mechanism for fluid reabsorption across the surface epithelium. However to date the contribution of fluid reabsorption by glandular epithelial cells has not been established. This study using reverse transcriptase polymerase chain reaction demonstrates the presence of several AQP isoforms in the rat uterus at the time of implantation while immunofluorescence data demonstrates an apical distribution of AQPs5 and 9 in the glandular epithelium at the time of implantation. The presence of AQPs5 and 9 in the apical plasma membrane of the glandular epithelium seen in this study provides a mechanism for transcellular fluid transport across these glandular epithelial cells similar to that seen in luminal epithelial cells. The reabsorption of glandular fluid via AQP channels may also regulate luminal fluid volume and be involved in the reduction in luminal fluid seen at the time of implantation.

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“…Whole villous tissue (*50 mg/well) was incubated in 24-well polystyrene tissue culture dishes in 2 mL of serum-free Dulbecco modified Eagle medium (DMEM; Life Technologies, Inc BLR, Grand Island, New York) containing 100 IU/mL penicillin, 100 mg/ mL streptomycin, 32 mg/mL gentamicin at 37 C for 24 hours in a humidified gas mixture of 5% CO 2 and 95% air. 6 Concentrations of hCG used in these experiments were determined by the biological activity, and each preparation was tested and confirmed by several assays before added in the tissue culture.…”

Section: Tissue Culturementioning

confidence: 99%

“…However, we have not been able to relate the higher AQP9 expression with its functionality for the transport of water and mannitol. 6 It is well-documented that altered placental villous angiogenesis and a poorly developed fetoplacental vasculature are present in preeclampsia. These abnormalities in trophoblastic invasion may induce modifications in the placental hormone profiles in the maternal circulation, thus leading to an impairment of the placental function.…”

Section: Introductionmentioning

confidence: 99%

High Levels of Human Chorionic Gonadotropin (hCG) Correlate With Increased Aquaporin-9 (AQP9) Expression in Explants From Human Preeclamptic Placenta

et al. 2010

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Trophoblastic abnormalities have a central role in the pathophysiology of preeclampsia, and some placental hormones, such as human chorionic gonadotropin (hCG), could affect the placental function. Here, we hypothesized that the elevated serum levels of hCG may be involved in the increased aquaporin-9 (AQP9) protein expression in preeclamptic placentas via adenosine 3('),5(')-cyclic phosphate (cAMP) pathways. Normal placental explants were cultured with different concentrations of recombinant hCG or 8-Br-cAMP, a potent analogue of cAMP. We evaluated AQP9 protein expression and localization. After both treatments, we localized AQP9 in the apical membrane of syncytiotrophoblast and in the cytoplasm. We also observed a concentration-dependent effect on AQP9 protein expression. In addition, water uptake increased 1.6-fold in explants treated with hCG. Our results suggest that hCG may increase AQP9 protein expression and functionality via cAMP pathways. Although, in preeclamptic placentas high levels of hCG may upregulate AQP9 protein expression, AQP9 functionality was reduced possibly by other factors.

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Functional and Molecular Expression of AQP9 Channel and UT-A Transporter in Normal and Preeclamptic Human Placentas

Cited by 61 publications

References 31 publications

Placental lesions associated with oligohydramnios in fetal growth restricted (FGR) pregnancies

Placental lesions associated with oligohydramnios in fetal growth restricted (FGR) pregnancies

Aquaporins are upregulated in glandular epithelium at the time of implantation in the rat

High Levels of Human Chorionic Gonadotropin (hCG) Correlate With Increased Aquaporin-9 (AQP9) Expression in Explants From Human Preeclamptic Placenta

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