Functional and Morphological Properties of Human Omental Resistance Vessels

Aalkjær, Christian; Mulvany, Michael J.

doi:10.1159/000158357

Cited by 13 publications

(11 citation statements)

References 16 publications

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“…To our knowledge, there are no corresponding studies in human mesenteric arteries. Although, a study in human omental arteries supports a normalization ratio of 0.9 (Aalkjaer and Mulvany ).…”

Section: Discussionmentioning

confidence: 98%

Translational value of mechanical and vasomotor properties of mouse isolated mesenteric resistance‐sized arteries

Outzen

Zaki

Abdolalizadeh

et al. 2015

Pharmacology Res & Perspec

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Mice are increasingly used in vascular research for studying perturbations and responses to vasoactive agents in small artery preparations. Historically, small artery function has preferably been studied in rat isolated mesenteric resistance‐sized arteries (MRA) using the wire myograph technique. Although different mouse arteries have been studied using the wire myograph no establishment of optimal settings has yet been performed. Therefore, the purposes of this study were firstly to establish the optimal settings for wire myograph studies of mouse MRA and compare them to those of rat MRA. Second, by surveying the literature, we aimed to evaluate the overall translatability of observed pharmacological vasomotor responses of mouse MRA to those obtained in rat MRA as well as corresponding and different arteries in terms of vessel size and species origin. Our results showed that the optimal conditions for maximal active force development in mouse MRA were not significantly different to those determined in rat MRA. Furthermore, we found that the observed concentration‐dependent vasomotor responses of mouse MRA to noradrenaline, phenylephrine, angiotensin II, sarafotoxin 6c, 5‐hydroxytryptamine, carbachol, sodium nitroprusside, and retigabine were generally similar to those described in rat MRA as well as arteries of different sizes and species origin. In summary, the results of this study provide a framework for evidence‐based optimization of the isometric wire myograph setup to mouse MRA. Additionally, in terms of translational value, our study suggests that mouse MRA can be applied as a useful model for studying vascular reactivity.

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Section: Discussionmentioning

confidence: 98%

Translational value of mechanical and vasomotor properties of mouse isolated mesenteric resistance‐sized arteries

Outzen

Zaki

Abdolalizadeh

et al. 2015

Pharmacology Res & Perspec

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“…In previous studies on the effect of noradrenaline in small resistance arteries (also approximately 200 pm in diameter), Aalkjzr et al found pD2 values of 6.43 in human omental arteries and 6.55 in subcutaneous vessels (1,2), and Nyborg and Mikkelsen found a pD2 of 5.96 in rat mesenteric arteries (27). Our finding of a pD, of 6.95 in bone thus suggests that bone arteries are quite sensitive to noradrenaline.…”

Section: Smooth-muscle Functionmentioning

confidence: 97%

Method for assessment of vascular reactivity in bone: In vitro studies on resistance arteries isolated from porcine cancellous bone

Lundgaard¹,

Aalkjær

Holm-Nielsen

et al. 1996

Journal Orthopaedic Research

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Knowledge about vascular regulation in bone is central to the understanding of both normal and pathological bone physiology. This article describes a new method for direct assessment of the reactivity of bone blood vessels. Resistance arteries (diameter approximately 250 microns) were isolated from epiphyseal cancellous bone (porcine femoral condyle). Arterial segments (2 mm long) were mounted as ring preparations on a myograph, and isometric force development was measured continuously. Fifty-nine vessels from 31 pigs were investigated. The active force development was maximal at 0.9 x L100 in nine of 12 investigated arteries (L100 corresponds to the circumference the vessel would have if relaxed and exposed to a luminal pressure of 100 mm Hg [13.3 kPa]). In all subsequent experiments, the vessels were stretched to 0.9 x L100. Noradrenaline (2 x 10(-8) to 10(-5) M) induced a concentration-dependent vasoconstriction; mean maximal tension development was 3.69 N/m. This force development would enable the arteries to contract against a pressure of more than 22 kPa (165 mm Hg), indicating preserved function of the media smooth muscle. Response to acetylcholine (10(-7) to 10(-5) M) was observed in only two of 12 arteries. Bradykinin (10(-11) to 10(-6) M) induced a concentration-dependent and reproducible relaxation in all vessels; the relaxation was endothelium-dependent, since no effect of bradykinin was detected after mechanical removal of the endothelium. Sodium nitroprusside (10(-4) M) induced a reproducible and endothelium-independent vasorelaxation. The results demonstrate preserved function of both smooth muscle and endothelium in this preparation. The model allows pharmacological investigations of bone arteries under well defined conditions and enables studies on focal bone lesions and human bone tissue.

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“…Each vessel was then set to the normalized internal diameter, L " l 0.9iL "!! \π, at which contraction is thought to be optimal [26].…”

Section: Experimental Protocolmentioning

confidence: 99%

Effect of adrenomedullin on the production of endothelin-1 and on its vasoconstrictor action in resistance arteries: evidence for a receptor-specific functional interaction in patients with heart failure

et al. 2001

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Endothelin-1 (ET-1) and adrenomedullin (ADM) are both produced in the arterial wall, but have opposing biological actions. Evidence from experimental animals suggests a functional interaction between ET-1 and ADM. We have tested this in humans. Small resistance arteries were obtained from gluteal biopsies taken from patients with chronic heart failure (CHF) due to coronary heart disease (CHD), or with CHD and preserved ventricular function. The contractile responses to big ET-1 and to ET-1 in both sets of vessels were studied in the absence (control) and presence of ADM at 20 pmol/l (low ADM) or 200 pmol/l (high ADM), using wire myography. ADM did not affect the conversion of big ET-1 into ET-1 in vessels from patients with either CHD or CHF. Low ADM did not alter the contractile response to ET-1 in vessels from patients with CHF. Low ADM was not tested in vessels from patients with CHD, but high ADM did not affect this response in arteries from these patients. High ADM did, however, significantly reduce the vasoconstrictor effect of ET-1 in vessels from patients with CHF. The maximum response, as a percentage of the response to high potassium, was 199% (S.E.M. 25%) in the control experiments (n=14), 205% (27%) in the low-ADM (n=7) studies and 150% (17%) in the high-ADM (n=6) experiments (P<0.001). Furthermore, the Hill coefficient increased from 0.57+/-0.05 in the absence of ADM to 1.16+/-0.15 in the high-ADM experiments, indicating that ADM at 200 pmol/l specifically antagonized one receptor type in vessels from patients with CHF. We conclude that there is a one-site receptor interaction between ADM and ET-1 that is specific for vessels from patients with CHF. This functional interaction between ADM and ET-1 in resistance arteries may be of pathophysiological importance in CHF.

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Functional and Morphological Properties of Human Omental Resistance Vessels

Cited by 13 publications

References 16 publications

Translational value of mechanical and vasomotor properties of mouse isolated mesenteric resistance‐sized arteries

Translational value of mechanical and vasomotor properties of mouse isolated mesenteric resistance‐sized arteries

Method for assessment of vascular reactivity in bone: In vitro studies on resistance arteries isolated from porcine cancellous bone

Effect of adrenomedullin on the production of endothelin-1 and on its vasoconstrictor action in resistance arteries: evidence for a receptor-specific functional interaction in patients with heart failure

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