Functional and Morphological Response of In Vitro Lung and Myocardial Test Objects to Experimental Gas Environments

A system was constructed which alternately exposed cultured cells to specific concentrations of ozone in the gas phase and then to cell culture medium. It was designed to produce a constant mass transfer between the gas phase and the exposed cell phase. Monolayers of neonatal rat lung fibroblasts cultured in miniature glass dishes were used to test for ozone toxicity. The cells were alternately exposed to ozone (0.05, 0.15, 0.45, 1.35, and 4.05 ppm) for 30 sec and then to culture medium for 30 sec over a 1-hr period. Toxicity was measured as inhibition of cell growth 4 days after exposure to ozone. Growth was significantly inhibited at all concentrations with a 50% inhibitory concentration of 0.8 ppm. The system described was effective for quantifying ozone toxicity for cultured lung cells.

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An Ozone-Generating System and Chamber for Testing Injury to Cultured Cells

Wenzel

Kotek

Morgan

et al. 1979

Archives of Environmental Health: An International Journal

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An Ozone-Generating System and Chamber for Testing Injury to Cultured Cells

Wenzel

Kotek

Morgan

et al. 1979

Archives of Environmental Health: An International Journal

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Role of in vitro Factors in Ozone Toxicity for Cultured Rat Lung Fibroblasts

Wenzel

Morgan

1982

Drug and Chemical Toxicology

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Ozone toxicity for cultured rat lung fibroblasts was concentration dependent and was affected by the manner in which ozone was delivered to the cells, i.e. cultures were either rotated with a thin moving overlay of medium or were stationary with a fixed layer of medium between the cells and the gas phase. The influence of culture medium components and culture dish composition on the toxicity of ozone were also investigated. Cell viability, used to measure ozone toxicity, was quantified by the chromium-51 release assay, and by a viability index calculated from the percentage of cells stained with a vital dye combined with the decrease in cell number as determined by DNA measurements. During stationary ozone exposure, toxicity appeared to be mediated primarily by hydrogen peroxide and could be inhibited by catalase or fetal bovine serum when measured by the viability index. During rotated exposure, catalase and fetal bovine serum provided no protection when measured by the viability index, however, when measured by the chromium-51 release assay, fetal bovine serum was partially protective. The effect of ozone on the fibroblasts was not influenced by whether culture dishes were glass or plastic, or whether the culture medium was balanced salt solution or complete chemically-defined medium.

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Functional and Morphological Response of In Vitro Lung and Myocardial Test Objects to Experimental Gas Environments

Cited by 3 publications

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An Ozone-Generating System and Chamber for Testing Injury to Cultured Cells

An Ozone-Generating System and Chamber for Testing Injury to Cultured Cells

An Ozone-Generating System and Chamber for Testing Injury to Cultured Cells

Role of in vitro Factors in Ozone Toxicity for Cultured Rat Lung Fibroblasts

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