Functional and Neuromuscular Changes after Anterior Cruciate Ligament Rupture in Rats

Laurin, Jérôme; Pin-Barre, Caroline; Bernard, Gérôme; Dousset, Erick; Decherchi, Patrick

doi:10.1249/mss.0000000000000860

Cited by 5 publications

(7 citation statements)

References 38 publications

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“…Twenty-four rats in the OA (n ϭ 12) and OAϩPTH (n ϭ 12) groups received ACLT via medial parapatellar approach at 11 wk old with an average body weight of 396.8 g. The rats were positioned in supine position throughout the surgery, and the right knee stayed in flexed position to access the ACL. The ACL was severed near tibia insertion by a sharp scalpel under direct vision (20,32). To ensure that the ACL was completely transected, the anterior drawer test was manually performed on the knee to visualize more than half of the tibial plateau.…”

Section: Methodsmentioning

confidence: 99%

Parathyroid hormone-(1–34) ameliorated knee osteoarthritis in rats via autophagy

Chang

et al. 2018

Journal of Applied Physiology

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Anterior cruciate ligament (ACL) tear can lead to osteoarthritis (OA). However, parathyroid hormone (PTH)-(1-34) was found to alleviate OA progression in a papain-induced OA model. Autophagy is a protective mechanism in normal cartilage, and its aging-related loss is linked with chondrocyte death and OA. Thus we examined the roles of autophagy in PTH treatment in OA after ACL transection (ACLT). Thirty-six rats were randomized into three groups: control group, ACLT-induced OA (OA) group, and OA with intra-articular PTH-(1-34) treatment (OA+PTH) group. Weight-bearing and treadmill tests were evaluated. Cartilage matrix was determined by a histological evaluation of glycosaminoglycan (GAG), Osteoarthritis Research Society International (OARSI) score, chondrocyte apoptosis, and immunohistochemistry. Rats in the OA group had significantly decreased weight bearing and running endurance. The histological results indicated that GAG, collagen type II, and chondrocyte autophagy had decreased but that the OARSI score, terminal differentiation markers (collagen type X and Indian hedgehog), and chondrocyte apoptosis had increased in the OA group. Additionally, PTH-(1-34) treatment significantly improved weight bearing and treadmill endurance, preserved GAG and collagen type II, and reduced the OARSI score and terminal differentiation markers. Finally, PTH-(1-34) ameliorated chondrocyte apoptosis by regulating the expression of autophagy-related proteins, through reducing mechanistic target of rapamycin (mTOR) and p62 and enhancing microtubule-associated protein-1 light chain 3 (LC3) and beclin-1. Reconstructive surgery after ACL rupture cannot prevent OA occurrence. Intra-articular PTH-(1-34) treatment can alleviate OA progression after ACLT and histological molecular changes. Possible mechanisms are reducing chondrocyte terminal differentiation and apoptosis, with increasing autophagy. NEW & NOTEWORTHY Anterior cruciate ligament (ACL) tear can lead to osteoarthritis (OA). Intra-articular parathyroid hormone (PTH)-(1-34) significantly improved weight bearing and treadmill endurance, preserved glycosaminoglycan and collagen type II, and reduced Osteoarthritis Research Society International (OARSI) score and terminal differentiation. Finally, PTH-(1-34) ameliorated chondrocyte apoptosis by regulating the expression of autophagy-related proteins, through reducing mechanistic target of rapamycin (mTOR) and p62 and enhancing microtubule-associated protein-1 light chain 3 (LC3) and beclin-1. PTH-(1-34) can alleviate OA progression after ACL transection. Possible mechanisms are reducing chondrocyte terminal differentiation and apoptosis, with increasing autophagy.

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Section: Methodsmentioning

confidence: 99%

Parathyroid hormone-(1–34) ameliorated knee osteoarthritis in rats via autophagy

Chang

et al. 2018

Journal of Applied Physiology

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“…The location of the electrodes was never changed throughout recordings (between PRE- and POST-injection measurements). The reflex signal was referred to a ground electrode implanted in an inert tissue, amplified (2k), and filtered (30 Hz to 10 kHz) with a differential amplifier (P2MP ® , 5104B, Marseille, France) ( Laurin et al, 2016 ). The maximal peak-to-peak amplitude of the somatic reflex wave was determined by incrementally increasing stimulation intensity (by 0.01-mA increments) from 0.05 mA until there was no further wave amplitude increment.…”

Section: Methodsmentioning

confidence: 99%

“…Once the maximal somatic reflex wave was determined, a series of 20 reflexes were evoked at 1 Hz during 20 s to control reflex amplitude stability. After 15 min of rest to reduce muscular fatigue, the reflex amplitude was recorded before and after the intra-arterial injection (0.4 ml) of the 3 following combinations of metabosensitive afferent activators at doses fixed in accordance with previous works ( Thimm and Baum, 1987 ; Hanna and Kaufman, 2003 ; Light et al, 2008 ; Laurin et al, 2016 ): 10 mM of potassium chloride (KCl) and 25 mM LA (KCl 10-LA 25), 25 mM of LA and 50 μg/kg of adenosine triphosphate (LA 25-ATP), and 50 mM of KCl and 50 mM of LA (KCl 50-LA 50). The injection of these three combinations was randomized across animals to avoid habituation/sensitization effects.…”

Section: Methodsmentioning

confidence: 99%

“…The injection of these three combinations was randomized across animals to avoid habituation/sensitization effects. Specifically, 6 reflexes were evoked before (PRE-injection) and a series of 20 reflexes were elicited after the injections (POST-injection) ( Laurin et al, 2010 , 2016 ). Each of the 3 combinations was delivered every 12 min to ensure that muscular environment was returned to baseline condition ( Rybicki et al, 1985 ).…”

Section: Methodsmentioning

confidence: 99%

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Cerebral Ischemia Changed the Effect of Metabosensitive Muscle Afferents on Somatic Reflex Without Affecting Thalamic Activity

et al. 2018

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The purpose of the present study was to examine the contribution of group III and IV metabosensitive afferents at spinal and supraspinal levels in rats subjected to middle cerebral artery occlusion (MCAO) with reperfusion during the acute phase. Animals were randomized in Control (n = 23), SHAM (n = 18), MCAO-D1 (n = 10), and MCAO-D7 (n = 20) groups. Rats performed the Electrical Von Frey and the Adhesive removal tests before the surgery and at day 1 (D1), D3, and D7 after MCAO. Animals were subjected to electrophysiological recordings including the responses of group III/IV metabosensitive afferents to combinations of chemical activators and the triceps brachii somatic reflex activity at D1 or D7. The response of ventral posterolateral (VPL) thalamic nuclei was also recorded after group III/IV afferent activation. Histological measurements were performed to assess the infarct size and to confirm the location of the recording electrodes into the VPL. Behavioral results indicated that MCAO induced disorders of both mechanical sensibility and motor coordination of paretic forepaw during 7 days. Moreover, injured animals exhibited an absence of somatic reflex inhibition from the group III/IV afferents at D1, without affecting the response of both these afferents and the VPL. Finally, the regulation of the central motor drive by group III/IV afferents was modified at spinal level during the acute phase of cerebral ischemia and it might contribute to the observed behavioral disturbances.

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“…However, the paw weight-bearing can only be analyzed in static position periods during trials. Thereby, weight distribution functional deficits associated with trauma of central and/or peripheral nervous system were objectively identified [34][35][36] .…”

Section: Behavioral Tests Dynamic Weight-bearingmentioning

confidence: 99%

Total Knee Arthroplasty with a Ti6Al4V/PEEK Prosthesis on an Osteoarthritis Rat Model: Behavioral and Neurophysiological Analysis

Lecocq

Linares

Chaves‐Jacob

et al. 2020

Sci Rep

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Arthroplasty is a surgical procedure to restore the function of the joint of patient suffering from knee osteoarthritis. However, postoperative functional deficits are reported even after a rehabilitation program. In order to determine the origin of functional deficits of patient suffering from knee osteoarthritis and total knee arthroplasty, we developed a rodent model including a chemicallyinduced-osteoarthritis and designed a knee prosthesis (Ti6Al4V/PEEK) biomechanically and anatomically adapted to rat knee joint. Dynamic Weight-Bearing, gait kinematics, H-reflex from vastus medialis muscle and activities from metabosensitive III and IV afferent fibers in femoral nerve were assessed at 1 and 3 months post-surgery. Results indicate that knee osteoarthritis altered considerably the responses of afferent fibers to their known activators (i.e., lactic acid and potassium chloride) and consequently their ability to modulate the spinal sensorimotor loop, although, paradoxically, motor deficits seemed relatively light. On the contrary, results indicate that, after the total knee arthroplasty, the afferent responses and the sensorimotor function were slightly altered but that motor deficits were more severe. We conclude that neural changes attested by the recovery of the metabosensitive afferent activity and the sensorimotor loop were induced when a total knee replacement was performed and that these changes may disrupt or delay the locomotor recovery. Osteoarthritis (OA) is a common degenerative joint disease, characterized by a failure of cartilage self-repair. It originates from a stress occurring on the synovial joint tissues including joint cartilage, subchondral bone, ligaments, menisci, periarticular muscles, peripheral nerves or synovium and leads to inflammatory reaction 1-3. Mainly, OA involves hands and weight bearing joints, especially the knee which appears to be the most affected 4. The main symptoms of knee OA are pain and motor limitations related to joint deformity and weakness of surrounding muscles 1,5,6. When these symptoms become unsustainable, patients often resort to total knee arthroplasty (TKA) also called total knee replacement which is the gold standard treatment for end-stage knee OA.

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Functional and Neuromuscular Changes after Anterior Cruciate Ligament Rupture in Rats

Cited by 5 publications

References 38 publications

Parathyroid hormone-(1–34) ameliorated knee osteoarthritis in rats via autophagy

Parathyroid hormone-(1–34) ameliorated knee osteoarthritis in rats via autophagy

Cerebral Ischemia Changed the Effect of Metabosensitive Muscle Afferents on Somatic Reflex Without Affecting Thalamic Activity

Total Knee Arthroplasty with a Ti6Al4V/PEEK Prosthesis on an Osteoarthritis Rat Model: Behavioral and Neurophysiological Analysis

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