Functional and regenerative effects of local administration of autologous mononuclear bone marrow cells combined with silicone conduit on transected femoral nerve of rabbits

Trindade, Anelise Bonilla; Schestatsky, Pedro; Torres, Vítor Félix; Gomes, Cristiano Mauro Assis; Gianotti, Giordano Cabral; Paz, Ana Helena da Rosa; Terraciano, Paula Barros; Marques, Janete Maria Volpato; Guimarães, Karina Magano; Graça, Dominguita Lühers; Cirne-Lima, Elizabeth Obino; Contesini, Émerson Antônio

doi:10.1016/j.rvsc.2015.07.008

Cited by 5 publications

(5 citation statements)

References 23 publications

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“…This even proves better this work that, irrespective of the alignment performed in the surgery, the implant is able to help achieve a proper functional recovery. In addition, there are studies that shows for AUT, with bone marrow cells, even at 75 d there is no functional recovery and no improvement in nerve regeneration using rabbits [56]. The same is also shown for another study in 91 d with a 10 mm gap [57].…”

Section: Histological Evaluationmentioning

confidence: 56%

Nanocellulose for peripheral nerve regeneration in rabbits using citric acid as crosslinker with chitosan and freeze/thawed PVA

et al. 2021

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This work investigates peripheral nerve regeneration using membranes consisting of pure chitosan (CHI), which was further blended with nanofibrillated cellulose, with citric acid as crosslinker, with posterior addition of polyvinyl alcohol, with subsequent freeze thawing. Nanocellulose improves the mechanical and thermal resistance, as well as flexibility of the film, which is ideal for the surgical procedure. The hydrogel presented a slow rate of swelling, which is adequate for cell and drug delivery. A series of in vitro tests revealed to be non-toxic for neuronal Schwann cell from the peripheral nervous system of Rattus norvegicus, while there was a slight increase in toxicity if crosslink is performed—freeze-thaw. The in vivo results, using rabbits with a 5 mm gap nerve defect, revealed that even though pure CHI was able to regenerate the nerve, it did not present functional recovery with only the deep pain attribute being regenerated. When autologous implant was used jointly with the biomaterial membrane, as a covering agent, it revealed a functional recovery within 15 d when cellulose and the hydrogel were introduced, which was attributed to the film charge interaction that may help influence the neuronal axons growth into correct locations. Thus, indicating that this system presents ideal regeneration as nerve conduits.

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Section: Histological Evaluationmentioning

confidence: 56%

Nanocellulose for peripheral nerve regeneration in rabbits using citric acid as crosslinker with chitosan and freeze/thawed PVA

et al. 2021

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“…In a rabbit model, they investigated the repair of a 5 mm femoral nerve gap with a BMSC-seeded silicone tube. Although histological analysis showed no significant difference with the control group (saline), a functional assessment showed a tendency towards better recovery using BMSC-loaded NGCs [42].…”

Section: Bone Marrow-derived Stem Cells Promote Nerve Regeneration In...mentioning

confidence: 67%

“…Biocompatible artificial scaffolds, such as natural polymers [39], silicone tubes [42,43], and ePGFE (GoreTex ® ) tubes [41], are easy to produce and sterilize. However, depending on the location and size of the repaired nerve gap, they may be uncomfortable for the patient and need to be surgically extracted [43].…”

Section: Scaffold Materials Used For Nerve Guidance Graftsmentioning

confidence: 99%

Mesenchymal Stem Cells in Nerve Tissue Engineering: Bridging Nerve Gap Injuries in Large Animals

Lischer

Summa

Petrou

et al. 2023

IJMS

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Cell-therapy-based nerve repair strategies hold great promise. In the field, there is an extensive amount of evidence for better regenerative outcomes when using tissue-engineered nerve grafts for bridging severe gap injuries. Although a massive number of studies have been performed using rodents, only a limited number involving nerve injury models of large animals were reported. Nerve injury models mirroring the human nerve size and injury complexity are crucial to direct the further clinical development of advanced therapeutic interventions. Thus, there is a great need for the advancement of research using large animals, which will closely reflect human nerve repair outcomes. Within this context, this review highlights various stem cell-based nerve repair strategies involving large animal models such as pigs, rabbits, dogs, and monkeys, with an emphasis on the limitations and strengths of therapeutic strategy and outcome measurements. Finally, future directions in the field of nerve repair are discussed. Thus, the present review provides valuable knowledge, as well as the current state of information and insights into nerve repair strategies using cell therapies in large animals.

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“…To our knowledge, this was the first review to focus on different peripheral nerves and discuss the application of different The collagen tube achieved an improved recovery in vocalization, arytenoid cartilage angles, compound muscle action potentials, and regenerated fiber area than that of autograft; the drug delivery system had superior efficacy than autograft in nerve regeneration of RLN transection injury. This cell therapy did not improve regeneration of the femoral nerve, but there was a tendency for better functional recovery [185] PGSm: poly(glycerol sebacate methacrylate); ION: fUBM-ECM: fetal porcine urinary bladder extracellular matrix; SCs: Schwann cells; NPCs: neural precursor cells; LBD: laminin-binding domains; BDNF: brain-derived neurotrophic factor; GDNF: glial cell-derived neurotrophic factor; HAS-Br-NPs: human serum albumin nanoparticles conjugated with brimonidine; MABMC: mononuclear autologous bone marrow cells.…”

Section: Coated With Pei and Loaded With Vegf And Ngfmentioning

confidence: 80%

Advances in Peripheral Nerve Injury Repair with the Application of Nanomaterials

Zhu

Song

et al. 2022

Journal of Nanomaterials

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Peripheral nerve injury (PNI) is a relatively common disease caused by various circumstances, ultimately affecting the life quality of patients. Although existing medications and surgical interventions have particular benefits for nerve repair, more effective therapeutic strategies are urgently needed for various types of nerve injuries. Increasing investigations of nanomaterials have demonstrated their excellent biological properties, such as biocompatibility, permeability, degradability, high medicine loading efficacy, suitable mechanical properties, and broad applications in the biomedical field. Concerning peripheral nerve (PN) repair, nanomaterials with outstanding biological properties can be fitted as nerve conduits to provide support and guidance for PN regeneration and loaded with functional cells, cytokines, or specific medications to promote regenerative outcomes further. Almost all existing studies have focused on the application of different nanomaterials in PN repair, while the application of nanomaterials in different PN injuries has not been taken into account. This article outlines the application of nanomaterials in the medical field and the prevalent therapeutic strategies for PN repair. Importantly, it focuses on the application of nanomaterials in various PNI diseases, covering injuries of the sciatic nerve, cavernous nerve, facial nerve, median nerve, and more.

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Functional and regenerative effects of local administration of autologous mononuclear bone marrow cells combined with silicone conduit on transected femoral nerve of rabbits

Cited by 5 publications

References 23 publications

Nanocellulose for peripheral nerve regeneration in rabbits using citric acid as crosslinker with chitosan and freeze/thawed PVA

Nanocellulose for peripheral nerve regeneration in rabbits using citric acid as crosslinker with chitosan and freeze/thawed PVA

Mesenchymal Stem Cells in Nerve Tissue Engineering: Bridging Nerve Gap Injuries in Large Animals

Advances in Peripheral Nerve Injury Repair with the Application of Nanomaterials

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