Functional Antibody Arrays through Metal Ion‐Affinity Templates

Vega, Rafael A.; Maspoch, Daniel; Shen, Clifton K.‐F.; Kakkassery, Joseph J.; Chen, Benjamin J.; Lamb, Robert A.; Mirkin, Chad A.

doi:10.1002/cbic.200600271

Cited by 32 publications

(25 citation statements)

References 44 publications

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“…However the antibody-Protein A/G interaction can suffer instability under certain conditions. Organic molecules such as metal complexes [13] or calixarene derivatives [14] have also demonstrated their usefulness to immobilize the antibody with proper orientation avoiding chemical modification of the biomolecule. Unfortunately, in many cases these strategies are not always optimized and assessed for label-free detection in biological fluids.…”

Section: Introductionmentioning

confidence: 99%

Direct Detection of Protein Biomarkers in Human Fluids Using Site-Specific Antibody Immobilization Strategies

Soler

Estévez

Álvarez

et al. 2014

Sensors

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Design of an optimal surface biofunctionalization still remains an important challenge for the application of biosensors in clinical practice and therapeutic follow-up. Optical biosensors offer real-time monitoring and highly sensitive label-free analysis, along with great potential to be transferred to portable devices. When applied in direct immunoassays, their analytical features depend strongly on the antibody immobilization strategy. A strategy for correct immobilization of antibodies based on the use of ProLinker™ has been evaluated and optimized in terms of sensitivity, selectivity, stability and reproducibility. Special effort has been focused on avoiding antibody manipulation, preventing nonspecific adsorption and obtaining a robust biosurface with regeneration capabilities. ProLinker™-based approach has demonstrated to fulfill those crucial requirements and, in combination with PEG-derivative compounds, has shown encouraging results for direct detection in biological fluids, such as pure urine or diluted serum. Furthermore, we have implemented the ProLinker™ strategy to a novel nanoplasmonic-based biosensor resulting in promising advantages for its application in clinical and biomedical diagnosis.

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Section: Introductionmentioning

confidence: 99%

Direct Detection of Protein Biomarkers in Human Fluids Using Site-Specific Antibody Immobilization Strategies

Soler

Estévez

Álvarez

et al. 2014

Sensors

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“…Surfaces covered with calixarene derivatives have been demonstrated to capture Abs with a proper orientation [10,11]. In addition, Zn II metal complexes have been used for Ab adsorption [12,13]. Mercaptohexadecanoic acid (MHA) was patterned on a thin-film gold substrate followed by coordination of the carboxylic acid groups of MHA to Zn II ions.…”

Section: Physical Adsorptionmentioning

confidence: 99%

Trends and Perspectives in Immunosensors

2012

Antibodies Applications and New Developments

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Immunosensors are devices that comprise both a biomolecular recognition system, such as an antibody and its corresponding antigen, and a transducer to translate the high affinity and specific binding event into a physical signal.Antibodies are produced by an immunological response to the presence of a foreign substance called an antigen. Antibodies may be immobilised onto a variety of platforms including bulk planar surfaces and nanoparticles by either covalent or adsorption strategies. Different interfaces between the bio-components and the detector are available to monitor in 'real-time' the signal generated by biological interactions. The transducers detect, for example, the change in electron transfer, absorbance, fluorescence, refractive index, mass change or heat transfer as the antibody binds to the antigen of interest. Such analytical devices have allowed a wide range of analytes to be identified and quantified such as pathogens, toxins, environmental food contaminants and disease biomarkers.The demand for sensitive, rapid, 'on-site' techniques has taken advantage of the latest advances in microfluidics and nanotechnology. This chapter will highlight current trends in immobilisation, micro/nano-fluidics/ and transducers utilised. A number of examples outlining the exploitation of these elements in immunosensors and their successful applications will be described.

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“…Although the cited work did not include a relevant biological application, the potential of this type of virus nanopattern in single-cell level virology has been demonstrated by another study that reported on similar structures made by DPN. Namely, nanoarrays of antibody were printed and used for recognition-directed assembly of recombinant parainfluenza virus SV5 [87]. The virus-loaded chip could be used for microscopy analysis of pathogenic infectivity at the single-cell, single-particle level [88].…”

Section: Examples Of Applications In Biologymentioning

confidence: 99%

Nanobiochips

Valiokas

2011

Cell. Mol. Life Sci.

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The actual progress towards biological chip devices consisting of nanostructured functional entities is summarized. The practical aspects of molecular nanobiochips are discussed, including the main surface chemistry platforms, as well as conventional and unconventional fabrication tools. Several successful biological demonstrations of the first generation of nanobiochip devices (mainly, different nanoarrays) are highlighted with the aim of revealing the potential of this technology in life sciences, medicine, and related areas.

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Functional Antibody Arrays through Metal Ion‐Affinity Templates

Cited by 32 publications

References 44 publications

Direct Detection of Protein Biomarkers in Human Fluids Using Site-Specific Antibody Immobilization Strategies

Direct Detection of Protein Biomarkers in Human Fluids Using Site-Specific Antibody Immobilization Strategies

Trends and Perspectives in Immunosensors

Nanobiochips

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