Functional Aspects of Cell Response to Heat Shock

VYa, Alexandrov

doi:10.1016/s0074-7696(08)62408-0

Cited by 32 publications

(16 citation statements)

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“…Even plants growing in their natural distribution range may experience high temperatures that would be lethal in the absence of this rapid acclimation; thus, ability to acquire thermotolerance is likely of significant importance to plants. Furthermore, because plants can experience major diurnal temperature fluctuations, the acquisition of thermotolerance may reflect a more general mechanism that contributes to homeostasis of metabolism on a daily basis.Although acquired thermotolerance has been described and studied in plants for decades (Alexandrov, 1994), only a limited number of factors have been defined that contribute to the development of thermotolerance. Many studies have documented that heat shock protein (Hsp) synthesis is correlated with the acquisition of thermotolerance (for review, see Vierling, 1991).…”

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“…Although acquired thermotolerance has been described and studied in plants for decades (Alexandrov, 1994), only a limited number of factors have been defined that contribute to the development of thermotolerance. Many studies have documented that heat shock protein (Hsp) synthesis is correlated with the acquisition of thermotolerance (for review, see Vierling, 1991).…”

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confidence: 99%

“…In addition to causing protein denaturation, high temperature also alters membrane fluidity, can disrupt the overall balance of metabolic processes, and leads to oxidative stress (Dewey, 1989;Larkindale and Knight, 2002). Heat stress also affects the cytoskeleton as assessed by cytoplasmic streaming (Alexandrov, 1994). Acquired thermotolerance can be hypothesized to moderate these types of damage through the induction of protective factors that limit the extent of damage or by enhancing processes required for recovery from damage, although these mechanisms need not be mutually exclusive.…”

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ArabidopsishotMutants Define Multiple Functions Required for Acclimation to High Temperatures

2003

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Plants acquire thermotolerance to lethal high temperatures if first exposed to moderately high temperature or if temperature is increased gradually to an otherwise lethal temperature. We have taken a genetic approach to dissecting acquired thermotolerance by characterizing loss-of-function thermotolerance mutants in Arabidopsis. In previous work, we identified single recessive alleles of four loci required for thermotolerance of hypocotyl elongation, hot1-1, hot2-1, hot3-1, and hot4-1. Completed screening of M 2 progeny from approximately 2500 M 1 plants has now identified new alleles of three of these original loci, along with three new loci. The low mutant frequency suggests that a relatively small number of genes make a major contribution to this phenotype or that other thermotolerance genes encode essential or redundant functions. Further analysis of the original four loci was performed to define the nature of their thermotolerance defects. Although the HOT1 locus was shown previously to encode a major heat shock protein (Hsp), Hsp101, chromosomal map positions indicate that HOT2, 3, and 4 do not correspond to major Hsp or heat shock transcription factor genes. Measurement of thermotolerance at different growth stages reveals that the mutants have growth stage-specific heat sensitivity. Analysis of Hsp accumulation shows that hot2 and hot4 produce normal levels of Hsps, whereas hot3 shows reduced accumulation. Thermotolerance of luciferase activity and of ion leakage also varies in the mutants. These data provide the first direct genetic evidence, to our knowledge, that distinct functions, independent of Hsp synthesis, are required for thermotolerance, including protection of membrane integrity and recovery of protein activity/synthesis.Plants have many different mechanisms for surviving high temperatures in their environment, including long-term evolutionary adaptations of life history and morphology and shorter term avoidance or acclimation mechanisms involving, for example, leaf orientation, transpirational cooling, or alterations of membrane lipid composition (Berry, 1975; Turner and Kramer, 1980;Radin et al., 1994). It has also long been known that plants, like other organisms, have the ability to acquire thermotolerance rapidly, within hours, to otherwise normally lethal high temperatures (Alexandrov, 1961;Lin et al., 1984;Neumann et al., 1989; Vierling, 1991). The acquisition of thermotolerance is a cell autonomous phenomenon and results from prior exposure to a conditioning pretreatment, which can be a short, sublethal high temperature or other moderate stress treatments (Lindquist, 1986). Thermotolerance can also be induced by a gradual increase to the normally lethal temperature (Vierling, 1991), as would be experienced in the natural environment (Ansari and Loomis, 1959;Ehler, 1973). Even plants growing in their natural distribution range may experience high temperatures that would be lethal in the absence of this rapid acclimation; thus, ability to acquire thermotolerance is likely of significa...

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ArabidopsishotMutants Define Multiple Functions Required for Acclimation to High Temperatures

2003

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“…They perform important functions in the folding/unfolding and translocation of proteins as well as in the assembly/disassembly of protein complexes and the refolding of damaged proteins 17,18 . They also involve in both intracellular and extracellular immune functions 19 and apoptosis 20 .…”

Section: Introductionmentioning

confidence: 99%

“…The HSP superfamily includes a number of different molecular-weight-class families: HSP110, HSP90, HSP70, HSP60, HSP47, and a group of small HSP ranging from 16 to 40 kDa. They make up approximately 5-10% of the total protein content of cells under conditions of normal, healthy growth 19 and are expressed or increased in response to various biological stressors, such as heat 2,8,9 , malignant tumors 21 , inflammation 22 , viral infections and exposure to toxic chemicals 17,18 . These processes are also involved in the production of ROS, suggesting that oxidative stress is a key mechanism mediating HSP induction 23 .…”

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confidence: 99%

Effect of Vit E on Secretion of HSP-70 in Testes of Broilers Exposed to Heat Stress

Kapakin¹,

İmik²,

Gümüş³

et al. 2013

Kafkas Univ Vet Fak Derg

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The objective of this experiment was to investigate the ameliorative effect of vitamin E (Vit E) on tissue damage caused by heat stress in the testes of broilers, and to correlate this effect with the secretion of the HSP-70 protein. In the study totally 30 broilers (breed Ross 308) were used. Animals were divided into three groups, including the control group. Group Control (C); these broilers (24 o C) were not administered with Vit E in the diet and not subjected to heat stress. Group Stress (S); these broilers not administered with Vit E in the diet but subjected to heat stress (34 o C). Group Stress +Vitamin E (Group S + vit E); broilers subjected to heat stress (34 o C) and administered with vitamin E. Histopathological examination demonstrated the presence of degenerative alterations in testes tissue of the group S. However, there has been seen diminishing the severity of degeneration in the group S+Vit E. While secretion of HSP-70 was determined in the testes of all groups in varying degree, the highest secretion level of HSP-70 was in the group C, the lowest secretion level of HSP-70 was in the group S. It is concluded that heat stress reduces the secretion of HSP-70 in testes of broilers, when vitamin E is administered, the secretion of HSP-70 increases again and reduces tissues damage. Keywords: Heat stress, Broiler, Testis, Vit E, HSP-70 Sıcaklık Stresine Maruz Bırakılan Broilerlerin Testislerinde Vit E' in HSP-70 Sekresyonu Üzerine Etkisi ÖzetBu çalışmanın amacı broilerlerin testislerinde ısı stresinin sebep olduğu doku hasarı üzerine vitamin E (Vit E)'in iyileştirici etkisini araştırmak ve bu etkinin HSP-70 proteininin sekresyonu ile ilişkisini ortaya koymaktır. Çalışmada toplam olarak 30 adet (Ross 308 ırkı) broiler kullanıldı. Hayvanlar kontrol grubu da dahil olmak üzere üç gruba ayrıldı. Grup kontrol (C); sıcaklık stresine maruz bırakılmayan (24 o C) ve diyetlerine Vit E eklenmeyen broiler. Grup stress (S); diyetlerine Vit E eklenmeyen ancak sıcaklık stresine maruz bırakılan (34 o C) broiler. Grup Stres +Vitamin E (Grup S + vit E); sıcaklık stresine maruz bırakılan (34 o C) ve diyetlerine vitamin E eklenen broiler. Histopatolojik inceleme S grubunun testislerinde dejeneratif değişiklikler gösterdi. Ancak S + Vit E grubunda dejenerasyonun şiddetinin azaldığı gözlendi. Grupların tamamının testislerinde farklı seviyede HSP-70 sekresyonu saptanırken, HSP-70'in en yüksek seviyesi grup C'de en düşük seviyesi grup S'deydi. Isı stresinin broilerlerin testislerinde HSP-70 sekresyonunu azaltığı, vitamin E uygulandığında, HSP-70 sekresyonunun yeniden artığı ve doku hasarını azalttığı sonucuna varıldı.

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Application of Spin Labels To Membrane Bioenergetics

Тихонов

Subczynski

Biomedical EPR, Part A: Free Radicals, Metals, Medicine, and Physiology

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Functional Aspects of Cell Response to Heat Shock

Cited by 32 publications

References 279 publications

ArabidopsishotMutants Define Multiple Functions Required for Acclimation to High Temperatures

ArabidopsishotMutants Define Multiple Functions Required for Acclimation to High Temperatures

Effect of Vit E on Secretion of HSP-70 in Testes of Broilers Exposed to Heat Stress

Application of Spin Labels To Membrane Bioenergetics

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