Functional aspects of the subcommissural organ-Reissner's fiber complex with emphasis in the clearance of brain monoamines

Rodrı́guez, Sara; Caprile, Teresa

doi:10.1002/1097-0029(20010301)52:5<564::aid-jemt1041>3.0.co;2-7

Cited by 24 publications

(14 citation statements)

References 52 publications

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“…Indeed, one subpopulation of secretory cells of the SCO secretes transthyretin. The latter is a key protein involved in the transport of thyroid hormone and retinol in the CSF (Rodríguez and Caprile 2001). Interestingly, D 4 dopamine receptors are circumscribed to a subpopulation of ependymal cells of the bovine SCO (present report).…”

Section: Localization By Immunocytochemistrymentioning

confidence: 50%

“…Although the function of the SCO in adulthood is far from being fully known, the early view that it participates in the clearance of CSF monoamines (Hess and Sterba 1973) has gained strong support from recent investigations (Rodríguez et al 1999;Rodríguez and Caprile 2001;Caprile et al 2003). The elucidation of the mechanisms controlling the secretory activity of the SCO may contribute to the further unfolding of the overall function of this brain gland.…”

Section: Introductionmentioning

confidence: 99%

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The subcommissural organ expresses D2, D3, D4, and D5 dopamine receptors

Tomé¹,

Jiménez²,

Richter

et al. 2004

Cell Tissue Res

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Dopamine receptors have been found in certain populations of non-neuronal cells in the brain, viz., discrete areas of ciliated ependyma and the ependymal cells of the choroid plexus. We have studied the presence of both tyrosine-hydroxylase-immunoreactive nerve fibers and dopamine receptors in the subcommissural organ (SCO), an ependymal brain gland that is located in the roof of the third ventricle and that secretes, into the cerebrospinal fluid, glycoproteins that aggregate to form Reissner's fiber (RF). Antibodies against D2, D3, D4, and D5 dopamine receptors were used in immunoblots of bovine striatum, fresh SCO, and organ-cultured SCO, and in immunocytochemistry of the bovine, rat, and mouse SCO. Only a few tyrosine-hydroxylase fibers appeared to reach the SCO. However, virtually all the secretory ependymal and hypendymal cells of the SCO immunoreacted with antibodies against D2, D4, and D5 receptors, with the last-mentioned rendering the strongest reaction, especially at the ventricular cell pole of the secretory ependymocytes, suggesting that dopamine might reach the SCO via the cerebrospinal fluid. The antibodies against the four subtypes of receptors revealed corresponding bands in immunoblots of striatum and fresh SCO. Although the cultured SCO displayed dopamine receptors, dopamine had no apparent effect on the expression of the SCO-spondin gene/protein or on the release of RF-glycoproteins (SCO-spondin included) by SCO explants, suggesting that dopamine affects the function(s) of the SCO differently from the secretion of RF-glycoproteins.

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Section: Localization By Immunocytochemistrymentioning

confidence: 50%

Section: Introductionmentioning

confidence: 99%

The subcommissural organ expresses D2, D3, D4, and D5 dopamine receptors

Tomé¹,

Jiménez²,

Richter

et al. 2004

Cell Tissue Res

View full text Add to dashboard Cite

show abstract

“…The immunological blockade of the extracellular SCO secretory material has previously been achieved in rat embryos by maternal delivery of specific anti-RF antibodies through transplacental transport (S. Rodriguez et al 1999;Vio et al 2000;S. Rodriguez and Caprile 2001) and in adult rats by a single injection of anti-RF antibody into the CSF (S. Rodriguez et al 1990).…”

Section: Csf-injected Hybridoma Cells Synthesize and Release Antibodimentioning

confidence: 99%

Continuous delivery of a monoclonal antibody against Reissner’s fiber into CSF reveals CSF-soluble material immunorelated to the subcommissural organ in early chick embryos

et al. 2006

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The subcommissural organ (SCO) is an ependymal differentiation located in the dorsal midline of the caudal diencephalon under the posterior commissure. SCO cells synthesize and release glycoproteins into the cerebrospinal fluid (CSF) forming a threadlike structure known as Reissner's fiber (RF), which runs caudally along the ventricular cavities and the central canal of the spinal cord. Numerous monoclonal antibodies have been raised against bovine RF and the secretory material of the SCO. For this study, we selected the 4F7 monoclonal antibody based on its cross-reactivity with chick embryo SCO glycoproteins in vivo. E4 chick embryos were injected with 4F7 hybridoma cells or with the purified monoclonal antibody into the ventricular cavity of the optic tectum. The hybridoma cells survived, synthesized and released antibody into the CSF for at least 13 days after the injection. E5 embryos injected with 4F7 antibody displayed precipitates in the CSF comprising both the monoclonal antibody and anti-RF-positive material. Such aggregates were never observed in control embryos injected with other monoclonal antibodies used as controls. Western blot analysis of CSF from E4-E6 embryos revealed several immunoreactive bands to anti-RF (AFRU) antibody. We also found AFRU-positive material bound to the apical surface of the choroid plexus primordia in E5 embryos. These and other ultrastructural evidence suggest the existence of soluble SCO-related molecules in the CSF of early chick embryos.

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“…This view was widely accepted (Horsley, 1908), but later rejected after it was shown that the fiber lacks a polarized membrane characteristic of neural spikes. While the fiber is still excluded as a neural correlate of consciousness, and its function remains unknown, it has been associated with the regulation of the chemistry of the cerebrospinal fluid (Hess, Sterba, 1973;Rodriguez, 2001;Sterba, 1975) hydrocephalus (Rodriguez, 2007: Vio, 2000, and embryonic neurogenesis, morphogenesis and axonal guidance (Burstyn-Cohen, Frumkin, 1998;El-Bitar, Dastugue and Meiniel, 1999;Lehmann, Naumann, 2005).…”

Section: Reissner's Fibermentioning

confidence: 99%

The Broken Thread of Asian Culture

Wile¹

2018

AJSSS

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Sociobiology derives its atheistic stance from the Darwinian framework of purposeless, naturally selections of random variations of matter in motion. However, explanatory gaps in sociobiology’s explanation of religion, from the initial cosmic singularity to free will, invite a Divine foot in the door. By interpreting yogic, Taoist and Kabbalistic descriptions of the anatomical connection between the human and the divine not as primitive, poetic metaphors but as interoceptions of a little-known, enigmatic, epigenetically suppressed, structure running through the central axis of the central nervous system called Reissner’s fiber. I propose a new theistic sociobiological theory of religion. Justified belief in this theory could epigenetically reawaken the suppressed Reissner’s fiber genes and begin the empirical testing of the theory.

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Functional aspects of the subcommissural organ-Reissner's fiber complex with emphasis in the clearance of brain monoamines

Cited by 24 publications

References 52 publications

The subcommissural organ expresses D2, D3, D4, and D5 dopamine receptors

The subcommissural organ expresses D2, D3, D4, and D5 dopamine receptors

Continuous delivery of a monoclonal antibody against Reissner’s fiber into CSF reveals CSF-soluble material immunorelated to the subcommissural organ in early chick embryos

The Broken Thread of Asian Culture

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