Functional assessment of AtPAP17; encoding a purple acid phosphatase involved in phosphate metabolism in Arabidopsis thaliana

Langeroudi, Arash Jamali; Sabet, Mohammad; Jalali-Javaran, Mokhtar; Zamani, Katayoun; Lohrasebi, Tahmineh; Malboobi, Mohammad Ali

doi:10.1007/s10529-023-03375-x

Cited by 3 publications

(2 citation statements)

References 94 publications

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“…Alternatively, the lack of a severe P starvation response phenotype in the SlPAP17b -silenced plants could be due to the activation of some other PAPs compensating for its compromised function. This observation is well supported by the atpap17 mutant seedlings phenotype, where no change in the seedling phenotype of this mutant vis-à-vis wild-type plants has been reported (Jamali Langeroudi et al, 2023). In atpap17 mutant seedlings, the complementary induction of AtPAP26 has been reported to minimize Pi starvation response (Farhadi et al, 2020).…”

Section: Discussionsupporting

confidence: 65%

“…These two members have dual localization to the cell vacuole and extracellular matrix (O’Gallagher et al, 2022). Thus, both these genes are also secretory in nature (Farhadi et al, 2020; Hurley et al, 2010; Jamali Langeroudi et al, 2023; Robinson et al, 2012). Our search for the homologs of these genes identified two isoforms each for AtPAP17 ( SlPAP17a and SlPAP17b ) and AtPAP26 ( SlPAP26a and SlPAP26b ) in the tomato genome.…”

Section: Discussionmentioning

confidence: 99%

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Lack of Purple Acid PhosphataseSlPAP26bcompromises the phosphorus starvation response in tomato independent of SlPHR1 and SlPHL1

Akash,

Srivastava,

Roychowdhury

et al. 2023

Preprint

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The scarcity of soil phosphorus (P), an essential macronutrient, often limits plant growth and development. Enhanced secretion of intracellular and secretory acid phosphatases is essential to maintain cellular inorganic P (Pi) homeostasis in plants. Herein, using transcriptomics and proteomics approach, we observed upregulation of several purple acid phosphatases (PAPs), includingSlPAP1,SlPAP10b,SlPAP12,SlPAP15,SlPAP17b,SlPAP26a, andSlPAP26bin Pi-deficient tomato seedlings. Higher transcript levels ofSlPAP17bandSlPAP26bin the older senescing leaves than the younger leaves indicated active involvement of these PAPs in Pi remobilization. Subsequent detailed characterization ofSlPAP17b,SlPAP26a, andSlPAP26brevealed a prominent role ofSlPAP26bin Pi homeostasis. Silencing ofSlPAP26bled to an exacerbated P starvation response as these plants exhibited smaller shoots, lower soluble Pi, total P levels, and higher sucrose than their EV controls under Pi deprivation.SlPAP26b-silenced plants also showed misregulation of P starvation inducible genes such as phosphate transporters and glycerolipid remodellers, even under Pi-sufficient conditions. WhereasSlPAP26blevels were induced by external sucrose, its expression was found to be independent of the Myb class master regulators of P starvation response, SlPHR1 and SlPHL1. Altogether, this study identifies a prominent role ofSlPAP26bin the Pi compensation network in tomato seedlings.

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Lack of Purple Acid PhosphataseSlPAP26bcompromises the phosphorus starvation response in tomato independent of SlPHR1 and SlPHL1

Akash,

Srivastava,

Roychowdhury

et al. 2023

Preprint

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Production of fungal phytases in solid state fermentation and potential biotechnological applications

Singh,

Pragya,

Tiwari

et al. 2023

World J Microbiol Biotechnol

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SlPHL1 positively modulates acid phosphatase in response to phosphate starvation by directly activating the genes SlPAP10b and SlPAP15 in tomato

Liu,

Li,

Zhang

et al. 2024

Physiologia Plantarum

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Increased acid phosphatase (APase) activity is a prominent feature of tomato (Solanum lycopersicum) responses to inorganic phosphate (Pi) restriction. SlPHL1, a phosphate starvation response (PHR) transcription factor, has been identified as a positive regulator of low Pi (LP)‐induced APase activity in tomato. However, the molecular mechanism underlying this regulation remains to be elucidated. Here, SlPHL1 was found to positively regulate the LP‐induced expression of five potential purple acid phosphatase (PAP) genes, namely SlPAP7, SlPAP10b, SlPAP12, SlPAP15, and SlPAP17b. Furthermore, we provide evidence that SlPHL1 can stimulate transcription of these five genes by binding directly to the PHR1 binding sequence (P1BS) located on their promoters. The P1BS mutation notably weakened SlPHL1 binding to the promoters of SlPAP7, SlPAP12, and SlPAP17b but almost completely abolished SlPHL1 binding to the promoters of SlPAP10b and SlPAP15. As a result, the transcriptional activation of SlPHL1 on SlPAP10b and SlPAP15 was substantially diminished. In addition, not only did transient overexpression of either SlPAP10b or SlPAP15 in tobacco leaves increase APase activity, but overexpression of SlPAP15 in Arabidopsis and tomato also increased APase activity and promoted plant growth. Subsequently, two SPX proteins, SlSPX1 and SlSPX4, were shown to physically interact with SlPHL1. Moreover, SlSPX1 inhibited the transcriptional activation of SlPHL1 on SlPAP10b and SlPAP15 and negatively regulated the activity of APase. Taken together, these results demonstrate that SlPHL1‐mediated LP signaling promotes APase activity by activating the transcription of SlPAP10b and SlPAP15, which may provide valuable insights into the mechanisms of tomato response to Pi‐limited stress.

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Functional assessment of AtPAP17; encoding a purple acid phosphatase involved in phosphate metabolism in Arabidopsis thaliana

Cited by 3 publications

References 94 publications

Lack of Purple Acid PhosphataseSlPAP26bcompromises the phosphorus starvation response in tomato independent of SlPHR1 and SlPHL1

Lack of Purple Acid PhosphataseSlPAP26bcompromises the phosphorus starvation response in tomato independent of SlPHR1 and SlPHL1

Production of fungal phytases in solid state fermentation and potential biotechnological applications

SlPHL1 positively modulates acid phosphatase in response to phosphate starvation by directly activating the genes SlPAP10b and SlPAP15 in tomato

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