Functional assessment of autologous platelet-rich plasma (PRP) after long-term storage at −20 °C without any preservation agent

Hoşnuter, Mübin; Aslan, Cem; Isik, Daghan; Caliskan, Gorkem; Arslan, Banu; Durğun, Mustafa

doi:10.1080/2000656x.2016.1237956

Cited by 19 publications

(25 citation statements)

References 18 publications

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“…Similarly, Wen et al reported that the concentrations of VEGF, epidermal growth factor (EGF), FGF‐B, IGF‐1, and PDGF‐AA were not only sustained but also showed an increase after 7 days at a storage temperature of 22°C. However, Hosnuter et al found that the levels of EGF, VEGF, PDGF, IGF‐1, and TGF‐β1 were significantly decreased after 7 days in PRP stored at −20℃. In our study, with activation, the concentration of all growth factors (except for FGF‐B) were similar at each time point tested during 7 days of storage, regardless of storage temperature, with TGF‐β1 reaching its highest concentration on day 7.…”

Section: Discussionmentioning

confidence: 98%

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Effect of Storage Conditions and Activation on Growth Factor Concentration in Platelet‐Rich Plasma

Kim

Bae

Park

et al. 2019

Journal Orthopaedic Research

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This study aimed to evaluate growth factor concentration in platelet‐rich plasma (PRP) (leukocyte‐rich PRP) based on storage temperature, duration of storage, and method of activation. PRP samples were stored at 24℃ (room temperature group), 4℃ (refrigerator group), and −70℃ (deep‐freezer group). In each temperature, four aliquots were prepared based on the time of analysis (immediately, 1, 3, and 7 days after preparation). After storage, concentrations of platelet‐derived growth factor‐AA (PDGF‐AA), transforming growth factor‐β (TGF‐β), vascular endothelial growth factor (VEGF), insulin‐like growth factor‐1 (IGF‐1), and fibroblast growth factor‐basic (FGF‐B) were assessed with/without activation using Quantikine colorimetric sandwich immunoassay kits. PRP was activated with 10% Triton‐X for PDGF‐AA, VEGF, FGF‐B, IGF‐1 measurement and sonication for TGF‐β1 measurement. Without activation, PDGF‐AA concentration was highest on day 7 in the room temperature group. With activation, the concentration of PDGF‐AA was constant over the observation period at all temperatures. Without activation, the TGF‐β1 concentration remained negligible over the observation period at all temperatures. However, with activation, TGF‐β1 gradually increased to its highest concentration on day 7 at all temperatures. Over the observation period, VEGF and IGF‐1 concentrations were constant with and without activation at all temperatures. Without activation, FGF‐B concentration increased, with the highest concentration observed on day 7 in the deep‐freezer group. With activation, FGF‐B concentration decreased after day 1 in the room temperature group. Growth factor concentration in PRP differed significantly based on storage temperature, duration of storage, and method of activation. Appropriate storage conditions and activation are important to optimize its effects on desired clinical outcomes. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:777‐784, 2020

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Section: Discussionmentioning

confidence: 98%

“…While earlier studies [14][15][16][25][26][27] have examined growth factor concentration, data on the effect of various storage conditions on the concentration of various growth factors in PRP are limited. In addition, none of these studies compared the effect of storage and activation on growth factor concentrations in PRP.…”

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confidence: 99%

Effect of Storage Conditions and Activation on Growth Factor Concentration in Platelet‐Rich Plasma

Kim

Bae

Park

et al. 2019

Journal Orthopaedic Research

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“…Conditions affecting the quality and effects of PRP include: the age, sex, and pathophysiology of blood donors during PRP preparation; methods of drawing blood; anticoagulants; centrifugation time/speed/temperature; preservation method of PRP; interval and dose of use.…”

Section: Discussionmentioning

confidence: 99%

Application of standardized platelet‐rich plasma in elderly patients with complex wounds

Tian

Cheng

Chen

et al. 2019

Wound Repair Regeneration

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In recent years, autologous platelet-rich plasma (PRP) derivatives have been used widely in the regeneration and repair of tissue, but a standard definition and preparation method for PRP are lacking. We developed a standardized method using platelet indices as quality-control indicators for PRP preparation. Twenty-one elderly patients (9 males, 12 females) with complex wounds were treated with standardized platelet-rich plasma (S-PRP). The platelet count in PRP after the second centrifugation was 1,069-1,436 × 10 9 /L. We adjusted the platelet concentration in PRP after a second centrifugation to 1,000 × 10 9 /L according to a formula using platelet-poor plasma (PPP). The standardized preparation method that we developed gave S-PRP with a relatively uniform platelet concentration. The wounds of 21 patients showed accelerated healing after S-PRP treatment, and there were no obvious side effects during treatment. These data suggest that our preparation method of S-PRP, using platelet indices as quality-control indicators with platelet count of 1,000 × 10 9 /L could be used for the treatment of complex wounds in the elderly. The preparation method of S-PRP proposed in the present study may be a simple and effective method of PRP quality control.

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“…It also points to a fast and effective preparation protocol for patients with up to 189,000 platelets/mm 3 , with biological activity tested in vivo, aiming at the preparation of PRP. CHO, 2008;SERRANO et al, 2010;ROFFI et al, 2014;GERSCH et al, 2017;HOSNUTER et al, 2017;MOORE et al, 2017). Diferenças estatísticas foram consideradas quando p < 0.05.…”

Section: Agradecimentosmentioning

confidence: 99%

“…O PRP é obtido a partir de uma amostra de sangue venoso autólogo anticoagulada passando por um processo de centrifugação que pode envolver uma ou mais centrifugações para selecionar uma fração plasmática contendo uma maior concentração de plaquetas e menor seleção de eritrócitos (WEHLING et al, 2017). Há diferentes formas para a obtenção do PRP, e o concentrado de plaquetas pode ser classificado em PRP puro (P-PRP), eliminando os leucócitos no isolamento ou em PRP com alta concentração de leucócitos (L-PRP), o qual possui alta concentração de leucócitos (DOHAN EHRENFEST; RASMUSSON; ALBREKTSSON, 2009).O PRP pode ser preparado para armazenamento sob diversas formas como congelamento, criopreservação ou liofilização, podendo ter ou não adicionamento de solução de estabilização(GERSCH et al, 2017;HOSNUTER et al, 2017;PIETRAMAGGIORI et al, 2006;ROFFI et al;2014;CHO, 2008;SERRANO et al;2009;MOORE et al;.…”

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<b>Efeitos do plasma rico em plaquetas (PRP) fresco e liofilizado na regeneração de pele em ratos</b>

Campos¹

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CAMPOS, RA. Efeitos do Plasma Rico em Plaquetas (PRP) fresco e liofilizado no modelo de regeneração de pele em ratos [Effects of Platelet Rich Plasma (PRP) fresh and lyophilized on skin regeneration model in rats] 2018. 64f. Dissertação (Mestrado em Ciências) -Platelet Rich Plasma (PRP) is a blood cell derived product obtained by centrifugation, which contains a high concentration of growth factors in a small volume of plasma. This procedure has been used since the 1950s and has been widely used in various areas such as Orthopedics, Dentistry, Cardiology, Ophthalmology, Dermatology for use in chronic wound repair, especially in diabetic patients or patients with venous complications, mucosal wounds, connective tissues, pathologies of the musculoskeletal system, fracture surface, bone graft, among others. In scarring and deficient tissues, there is an impairment of the ideal levels of growth factors in the wound healing phases. Therefore, it is important to maintain adequate levels of growth factors in all the different stages of tissue repair. The preparation of PRP is a non-standard procedure, with variable results being observed in the literature, being able to be involved with the amount of growth factors and methods of preparation. Usually, more than one PRP application is required involving a new blood collection for each application in order to accelerate tissue regeneration. The blood collections are invasive for the patient, being expensive in time and labor in the operational procedure, implying in the increase of the cost. The early preparation of PRP with possibility of storage for use in future applications during treatment could be an important solution to avoid the discomfort of patients, who often no longer have a good quality of life.Therefore, the present study aimed to compare the difference in the healing process for seven days in a nude rat dorsal lesion model, treated with human fresh PRP or stored PRP (prepared by lyophilization and stored at room temperature for 15 days, from two different donors, and compared to the application of recombinant human BB platelet-derived growth factor (rhPDGF-BB), one of the most abundant growth factors in PRP. The results showed comparative efficacies in the treatments of wounds with rhPDGF-BB (positive control) and with fresh PRPs or room temperature stored PRP of both donors, indicating the possibility of collecting blood from the patient only once and storing the same until the reconstitution of the product on the day of application, allowing the offer of a

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Functional assessment of autologous platelet-rich plasma (PRP) after long-term storage at −20 °C without any preservation agent

Abstract: The growth factor levels on days 7 and 14 suggest that autologous PRP can be stored at -20 °C without preservative agents, although in vivo studies are required in order to evaluate the clinical efficacy of the detected growth factor levels.

Cited by 19 publications

References 18 publications

Effect of Storage Conditions and Activation on Growth Factor Concentration in Platelet‐Rich Plasma

Effect of Storage Conditions and Activation on Growth Factor Concentration in Platelet‐Rich Plasma

Application of standardized platelet‐rich plasma in elderly patients with complex wounds

<b>Efeitos do plasma rico em plaquetas (PRP) fresco e liofilizado na regeneração de pele em ratos</b>

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