Functional assessment of human femoral arteries after cryopreservation

Stanke, Françoise; Riebel, Danièle; Carmine, Sessa; Cracowski, Jean‐Luc; Caron, Françoise; Magne, Jean-Luc; Egelhoffer, Harald; Bessard, Germain; Devillier, Philippe

doi:10.1016/s0741-5214(98)70163-6

Cited by 32 publications

(12 citation statements)

References 35 publications

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“…Similar observations have been made in the recent studies of human arteries. Cryopreserved human internal mammary arteries and femoral arteries had both poorly preserved smooth muscle functions and endothelial functions [10,11]. Freeze substitution of cryopreserved blood vessels demonstrates high levels of extracellular ice formation [12,13].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the effect of partial vitrification on stress development in cryopreserved blood vessels

Steif

Palastro

Rabin

2007

Medical Engineering & Physics

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Thermal stress development in blood vessels, during processes associated with vitrification (vitreous means glassy in Latin), is studied. This paper addresses the limiting case where the specimen completely crystallizes, while the cryoprotectant medium (CPA) completely vitrifies. This case is expected to provide upper boundary estimates for stresses for the more common problem of a partially vitrified sample. The CPA is modeled as a linear viscoelastic medium, with viscosity increasing exponentially with decreasing temperature; given the assumption of complete crystallization, the blood vessel is modeled as linear elastic below the freezing temperature. Consistent with previous observations, the CPA is found to behave linear elastically below a set-temperature, at which point the viscosity rises sufficiently quickly with further cooling. This observation reduces computational efforts and allows for parametric studies based on suitably chosen wholly elastic models. Both 2D concentric cylinder models of the blood vessel in a straight configuration and a 3D model of the vessel curled in a vial of CPA are studied; 2D models are shown to bound the results of the more general 3D problem. It is found that stress in the CPA decreases with increase in CPA volume, at least under conditions where the temperature can be viewed as uniform. Planar cracks are predicted to form transverse to the vessel axis, and to propagate right up to the blood vessel wall. Should such cracks propagate into the vessel, even over only a few μm, the mechanical damage to the lumen, or to endothelial cells, may cause the blood vessel to completely loose its functionality at the end of the cryopreservation protocol.

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Section: Introductionmentioning

confidence: 99%

Analysis of the effect of partial vitrification on stress development in cryopreserved blood vessels

Steif

Palastro

Rabin

2007

Medical Engineering & Physics

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“…In cryopreserved human internal mammary arteries and femoral arteries, both smooth muscle functions and endothelial functions were poorly-preserved. 10,25 Classical cryopreservation, with low concentrations DMSO, does a reasonable job of cell preservation by preventing intracellular ice formation; but it is a very poor method for preserving tissue. Even when all major cryopreservation variables are controlled, there is a limit, largely a function of tissue volume and geometry, beyond which traditional cryopreservation methods do not work consistently.…”

Section: Introductionmentioning

confidence: 99%

Thermal Expansion of Blood Vessels and Muscle Specimens Permeated with DMSO, DP6, and VS55 at Cryogenic Temperatures

Rabin

Plitz

2005

Ann Biomed Eng

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As part of an ongoing effort to characterize the continuum mechanics during cryopreservation via vitrification (vitreous in Latin means glass), the current study focuses on measuring the thermal expansion of cryoprotective agents in the presence and absence of biological samples of bovine muscle and goat arteries. The cryoprotectants under investigation are 7.05 M DMSO, DP6, and VS55, with permeation time of up to 48 h. This study focuses on the upper part of the cryogenic temperature range, where the cryoprotectant behaves like a liquid in all practical applications. The specific lower boundary of this range is unique to the cryoprotectant composition and to the applied cooling rate. The measurement device used in the current study is a modification of a prototype presented recently, in which modifications are made to accommodate biological samples. Based on thermal strain observations, it is concluded that the presence of tissue samples promotes crystallization (the tissue samples serve as potential nucleation sites). It is shown in this study that the thermal strain of tissue samples permeated with 7.05 M DMSO and DP6 is no greater than the thermal expansion of the same cryoprotectants in the absence of tissue samples, and in some cases it is significantly less. For a given cryoprotectant in comparable thermal conditions, experiments on bovine muscle samples show significantly higher scattered data than experiments on artery samples.

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“…A reduction of the postthaw contractility for KCl to less than 40% of fresh tissue has been observed in human coronary [7], internal mammary, [8] and human femoral arteries [4], whereas the maximal response to norepinephrine was reduced to 75% in human mesenteric arteries [5], to 24% for human internal mammary arteries, [5] or to 7% in human femoral arteries [4]. Freezing of vascular preparations generally results in a loss of contractile responsiveness [4,9,10]. In addition, Ku et al have shown that an identical cryopreservation procedure applied to canine and human coronary arteries resulted in a greater loss of contractility in human arteries, indicating vessel to vessel variability in the susceptibility to cryoinjury [11,12].…”

Section: Discussionmentioning

confidence: 92%

“…The maximal contractile responses were in the range of 13 to 27% of the responses in unfrozen pig aortas with noradrenaline and also with KCl, which provokes a contraction strictly dependent on Ca 2ϩ influx through voltage-operated channels. A reduction of the postthaw contractility for KCl to less than 40% of fresh tissue has been observed in human coronary [7], internal mammary, [8] and human femoral arteries [4], whereas the maximal response to norepinephrine was reduced to 75% in human mesenteric arteries [5], to 24% for human internal mammary arteries, [5] or to 7% in human femoral arteries [4]. Freezing of vascular preparations generally results in a loss of contractile responsiveness [4,9,10].…”

Section: Discussionmentioning

confidence: 98%

“…Improvements of the preservation techniques, such as the introduction of cryoprotectant agents and a controlled rate of freezing, have enabled the development of human blood vessel banks [4]. It has been seen that the postthaw functional recovery of cryopreserved isolated blood vessels is generally associated with reduced contractile force and endothelial function [4 -6].…”

Section: Introductionmentioning

confidence: 99%

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