Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Hempstock, Wendy; Ishizuka, Noriko; Hayashi, Hisaysohi

doi:10.3791/62468-v

Cited by 2 publications

(3 citation statements)

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“…Ussing chamber is commonly used to study the transport of epithelial nutrients and electrolytes in vitro. The short‐circuit current ( I sc ) or mucosal‐to‐serosal fluxes ( J ms ) induced by the addition of nutrients to the apical chamber of tissues, or epithelial cells would represent the process of nutrient absorption 29 . Using the electrophysiological measurements requires nutrient uptake accompanied by ion transmembrane transport, such as amino acids and glucose uptake accompanied by Na + and dipeptide transport accompanied by H + .…”

Section: Methods For Studying Intestinal Nutrient Absorptionmentioning

confidence: 99%

Ion channels and transporters regulate nutrient absorption in health and disease

Lu,

Luo,

et al. 2023

J Cellular Molecular Medi

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Ion channels and transporters are ubiquitously expressed on cell membrane, which involve in a plethora of physiological process such as contraction, neurotransmission, secretion and so on. Ion channels and transporters is of great importance to maintaining membrane potential homeostasis, which is essential to absorption of nutrients in gastrointestinal tract. Most of nutrients are electrogenic and require ion channels and transporters to absorb. This review summarizes the latest research on the role of ion channels and transporters in regulating nutrient uptake such as K+ channels, Ca2+ channels and ion exchangers. Revealing the mechanism of ion channels and transporters associated with nutrient uptake will be helpful to provide new methods to diagnosis and find potential targets for diseases like diabetes, inflammatory bowel diseases, etc. Even though some of study still remain ambiguous and in early stage, we believe that ion channels and transporters will be novel therapeutic targets in the future.

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Section: Methods For Studying Intestinal Nutrient Absorptionmentioning

confidence: 99%

Ion channels and transporters regulate nutrient absorption in health and disease

Lu,

Luo,

et al. 2023

J Cellular Molecular Medi

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show abstract

“…The intestinal preparations were mounted in Ussing chambers with a window diameter of 5 mm. As described previously [35], the intestinal preparation was bathed in HEPES buffer (pH 7.4, 37°C, gassed with 100% O2) containing in mM: 10 HEPES, 150…”

Section: Dilution Potential Measurementmentioning

confidence: 99%

“…and the impact of claudin-15 deletion on the permselectivity of the cecum and colon is unknown, we next measured the relative permeabilities of Na + (PNa) or Cl -(PCl) and the dilution potential of isolated segments of the cecum and large intestine using Ussing chambers. Dilution of the M (mucosal) side of the chamber in open circuit conditions, results in the influx of NaCl from the S (serosal) side, which results in change of membrane potential difference (PD)[34,35].Upon dilution of the M side, the change in PD of the cecum (1.4 (0.8) mV and 6.0 (2.7) mV, KO vs WT, p=0.0138) and the middle large intestine (1.3 (0.7) mV and 4.6(1.7) …”

mentioning

confidence: 99%

The effect of claudin-15 deletion on cationic selectivity and transport of paracellular pathways in the cecum and large intestine

Hempstock

Nagata

Ishizuka

et al. 2022

Preprint

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The large intestine plays a pivotal role in water and electrolyte balance. It is thought that paracellular transport plays a role in water and ion transport mechanisms in the cecum and large intestine, however, these mechanisms and the physiological roles have rarely been studied. Claudin-15 forms a Na+ channel in tight junctions in the small intestine but its role in the cecum and colon has not been investigated. This study aimed to explore physiological roles of claudin-15 in the mouse cecum and colon using claudin-15 (Cldn15) KO mice. Transmural electrical conductance, current, flux of Na+, and dilution potential were assessed in isolated preparations of cecum and colon from Cldn15 KO mice mounted in Ussing chambers. The induced current of short-chain fatty acids, which are fermentative products in the cecum and colon, was also measured. In the cecum but not the middle large intestine, electrical conductance and paracellular Na+ flux were decreased, while in both the cecum and the colon, paracellular Na+ permeability was decreased in Cldn15 KO mice. These results suggest that claudin-15 is the molecule responsible for cation selectivity in tight junctions of the cecum and colon, and decreased cation selectivity in the cecum may cause impaired absorption function.

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Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Cited by 2 publications

References 0 publications

Ion channels and transporters regulate nutrient absorption in health and disease

Ion channels and transporters regulate nutrient absorption in health and disease

The effect of claudin-15 deletion on cationic selectivity and transport of paracellular pathways in the cecum and large intestine

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