Functional Bioactivities of Soluble Seed Proteins from Two Leguminous Seeds

Abstract

Storage proteins from Sphenostylis stenocarpa and Phaseolus lunatus were fractionated, and their in vitro bioactivities were investigated. Albumin, globulin, prolamin, and glutelin constituents of the respective seeds were successively fractionated using the modified Osborne method. Phenylmethylsulfonyl fluoride (1 mM) was used as a protease inhibitor. The antioxidant, anti-inflammatory, and acetylcholinesterase-inhibitory activities of the protein fractions were evaluated using different appropriate techniques. Globulin was the predominant fraction, with a yield of 43.21±0.01% and 48.19±0.03% for S. stenocarpa and P. lunatus , respectively, whereas prolamin was not detected in both seeds. The protein fraction markedly scavenges hydroxyl radicals, nitric oxide radicals, and 2,2-diphenyl-1-picryldydrazyl radicals with concomitant high free radical-reducing power. Albumin and globulin fractions elicited the highest acetylcholinesterase-inhibitory potential of 48.75% and 49.75%, respectively, indicating their great application potential in managing neurodegenerative diseases. In this study, the albumin, globulin, and glutelin fractions of these underutilized legumes showed great analeptic bioactivities, which could be utilized as health-promoting dietary supplements/products.

Physicochemical Properties and Sensory Attributes of Yanggaeng Treated with Citrus Peel Powder

“…To evaluate the antioxidative attributes of Yanggaeng, 2.5 g of Yanggaeng was dissolved in 50 mL of 70% ethanol and incubated for 24 h. In succession, Yanggaeng was separated by centrifugation at 3000× g for 10 min. Quantification of total polyphenols, total flavonoids, scavenging activity against DPPH and ABTS radicals, and FRAP were determined following established protocols [17,[26][27][28][29]. To measure the DPPH and ABTS radical scavenging activity, DPPH solution (300 µM, Sigma-Aldrich Co., St. Louis, MO, USA) and ABTS solution (7 mM, Sigma-Aldrich Co.) were separately added to the sample solution.…”