Functional brain fluorescence plurimetry in rat by implantable concatenated CMOS imaging system

Abstract

Measurement of brain activity in multiple areas simultaneously by minimally invasive methods contributes to the study of neuroscience and development of brain machine interfaces. However, this requires compact wearable instruments that do not inhibit natural movements. Application of optical potentiometry with voltage-sensitive fluorescent dye using an implantable image sensor is also useful. However, the increasing number of leads required for the multiple wired sensors to measure larger domains inhibits natural behavior. For imaging broad areas by numerous sensors without excessive wiring, a web-like sensor that can wrap the brain was developed. Kaleidoscopic potentiometry is possible using the imaging system with concatenated sensors by changing the alignment of the sensors. This paper describes organization of the system, evaluation of the system by a fluorescence imaging, and finally, functional brain fluorescence plurimetry by the sensor. The recorded data in rat somatosensory cortex using the developed multiple-area imaging system compared well with electrophysiology results.

CMOS-Based Optoelectronic On-Chip Neural Interface Device

“…An optical imaging function can be used to observe the shape of the measurement target placed directly on the sensor surface. Although no optics such as lenses are integrated with the sensor's surface, we can obtain optical images of the target cells or tissues [15]- [17].…”

CMOS image sensor-based implantable glucose sensor using glucose-responsive fluorescent hydrogel

“…We have previously developed implantable CMOS image sensors for biomedical observation platforms, especially for functional and molecular imaging of the brain [10][11][12]. In this work, we use the same kind of sensor to detect the fluorescence of the glucoseresponsive hydrogel.…”

Recent Progress in Voltage-Sensitive Dye Imaging for Neuroscience

“…1 12 13 Most in vivo studies have been made in anesthetized 16 17 and sometimes freely moving rodents. [18][19][20][21] VSDi experiments on visual cortex have been performed in cats 22 and monkeys. 23 24 VSDi signals are much difficult to be measured from in vivo mammalian brain compared to in vitro targets, because of the noise from the cardiac rhythm and respiratory movements.…”