Functional cell models of the gut and their applications in food microbiology — A review

Cencič, Avrelija; Langerholc, Tomaž

doi:10.1016/j.ijfoodmicro.2010.03.026

Cited by 151 publications

(125 citation statements)

References 93 publications

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“…IPEC-J2 behave similarly to human colon adenocarcinoma cells (Caco-2 and T84 cells) with the advantage of not being cancerous, and their glycolysation pattern, proliferation rate and colonization ability are closer to physiological functioning of enterocytes 29 . Influenza A viruses in pigs can lead to both economic losses and can present permanent risks of cross-species transmission of new strains to humans 30 .…”

Section: Discussionmentioning

confidence: 93%

In vitro characterization of TMPRSS2 inhibition in IPEC-J2 cells

Pászti-Gere

Czimmermann

Ujhelyi

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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The transmembrane serine protease, TMPRSS2 is an important target in the treatment of seasonal influenza infections and contributes to prostate carcinogenesis and metastasis. In this study, the effect of the synthetic TMPRSS2 inhibitor I-432 on jejunal IPEC-J2 cell monolayers cultured on membrane inserts was characterized. Using a fluorogenic substrate, it was found that the apical addition of I-432 could suppress trypsin-like activity in the supernatants of IPEC-J2 cells. The inhibition of TMPRSS2 did not affect physiologically produced hydrogen peroxide levels in the apical and in basolateral compartments. Loss of expression of the TMPRSS2 serine protease domain (28 kDa) was also observed when cells were pre-exposed to I-432. Partial decrease in immunofluorescent signal intensities derived from the altered distribution pattern of TMPRSS2 was detected after a 48 h long incubation of IPEC-J2 cells with the inhibitor indicating the efficacy of TMPRSS2 inhibition via I-432 administration in vitro.

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Section: Discussionmentioning

confidence: 93%

In vitro characterization of TMPRSS2 inhibition in IPEC-J2 cells

Pászti-Gere

Czimmermann

Ujhelyi

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Several normal and transformed cell lines are used in food science (Cencic and Langerholc 2010 ). Caco-2, T84, HT-29, HUTU-80 and SW620 are the most widely used human intestinal cell lines.…”

Section: Relevance To Human In Vivo Situationmentioning

confidence: 99%

The IPEC-J2 Cell Line

Vergauwen

2015

The Impact of Food Bioactives on Health

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IPEC-J2 cells are intestinal porcine enterocytes isolated from the jejunum of a neonatal unsuckled piglet. The IPEC-J2 cell line is unique as it is derived from the small intestine and is neither transformed nor tumorigenic in nature. IPEC-J2 cells mimic the human physiology more closely than any other cell line of nonhuman origin. Therefore, it is an ideal tool to study epithelial transport, interactions with enteric bacteria, effects of probiotic microorganisms and the effect of nutrients and other feedstuffs on a variety of widely used parameters (e.g. transepithelial electrical resistance (TEER), permeability, metabolic activity) refl ecting epithelial functionality.IPEC-J2 cells undergo in culture a process of spontaneous differentiation that leads to the formation of a polarized monolayer with low or high TEER, depending on the type of serum added to the culture medium, within 1-2 weeks. Porcine serum gives rise to low resistance and normal active transport rates, enabling comparison with the in vivo situation. The high resistance caused by fetal bovine serum can be benefi cial to use when investigating compounds having a negative effect on the monolayer permeability or tight junction structures.There are still many opportunities for exploring the use of these cells as the available research is limited. This chapter will cover the origin, characteristics and methods of the use of IPEC-J2 cells as an in vitro model for several research applications, as well as comparisons between IPEC-J2 cells and other epithelial cell lines.

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“…Porcine small intestinal cell line (CLAB) and porcine blood macrophage (POM2) cell lines were used in the assays [33] Cells were routinely grown in Advanced Dulbecco's modified Eagle's medium (DMEM advanced) (Sigma-Aldrich, USA), supplemented with 5% Foetal Calf Serum (FCS) (Biowhittaker, Europe), L-glutamine (2 mM/L), penicillin (100 units/mL) and streptomycin (1mg/mL) at 37°C in humidified 5% CO 2 atmosphere in tissue culture flasks. Upon confluency, cell were passaged.…”

Section: Cell Culturementioning

confidence: 99%

Antiviral Activity of Selected Indian Medicinal Herbs Against Hepatitis E Virus (Hev) in the Established Porcine Cell Model

Roy¹,

Kanwar²,

Langerholc³

2017

J Res Educ Indian Med - ISSN 0970-7700

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Abstract:Background: It is known that hepatitis E virus (HEV) is zoonotically transmitted to humans and while other forms of hepatitis have been in focus for drug and vaccine development, HEV related mortality in case of pregnant women can reach upto 20%. Methods: HEV proteins, were successfully extracted from 22 pig stool samples and the presence of HEV capsid antigen encoded by the open reading frame 2 (ORF2) was confirmed using dot blot and western blotting in 4 samples. HEV samples were inoculated in pig epithelial cells (CLAB) and pig macrophage (POM2) to develop an in vitro model for observing the cytopathic effects (CPE). The HEV infected cell cultures were subjected to water: methanol (95:5) extracts from Kaempfaria galanga (Galangal), Mimosa pudica (Touch me not), Coleus aromaticus (Doddapatre)and Paederia foetida (Stinkvine).Results:We showed that incubation of 56 μg/mL HEV samples for 3 hours induced characteristic growth patterns in cells until 4 passages. We also found that extracts of K. galanga significantly (p<0.001) increased cell viability in CLAB cells while other extracts showed cytotoxic effects. In the preventive study (treatments of cells with extracts followed by infection of cells with viral proteins), both 10 -2 and 10 -3 dilutions of K. galanga extracts significantly (p<0.001) prevented a decrease in cell viability of CLAB cells after HEV infection. The 10 -2 dilution of C. aromaticus (p<0.01) and 10 -3 dilution of K. galanga was the only effective (p<0.001) extract in case of HEV infected POM2 cells. Conclusion: A new in vitro cell culture model based on CLAB and POM2 cells has been developed to study HEV.We are also the first to report the activity of these medicinal extracts against HEV in a cell culture model. Extracts of K. galanga and P. foetida showed a promising anti-viral activity and the potential to be used as a natural therapy against HEV.

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Functional cell models of the gut and their applications in food microbiology — A review

Cited by 151 publications

References 93 publications

In vitro characterization of TMPRSS2 inhibition in IPEC-J2 cells

In vitro characterization of TMPRSS2 inhibition in IPEC-J2 cells

The IPEC-J2 Cell Line

Antiviral Activity of Selected Indian Medicinal Herbs Against Hepatitis E Virus (Hev) in the Established Porcine Cell Model

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