Functional characterisation and genomic analysis of an epithelial calcium channel (ECaC) from pufferfish, Fugu rubripes

Qiu, Andong; Högstrand, Christer

doi:10.1016/j.gene.2004.07.041

Cited by 78 publications

(61 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite extensive examination of the zebrafish (Danio rerio) genome, it has not been possible to identify more than a single ECaC gene located on chromosome 16. A similar conclusion was reached following analysis of the pufferfish genome (Qiu and Hogstrand, 2004). These results are consistent with the phylogenetic analysis presented here (Fig.·3) and suggest that unlike in mammals, there is but a single gene for ECaC in rainbow trout, zebrafish, pufferfish and presumably other fish species.…”

Section: Discussionsupporting

confidence: 90%

“…See Table·2 for the description of genes used in this tree; c, chicken; cf, crayfish; d, dog; f, Fugu; h, human; m, mouse; r, rat; rb, rabbit; rt, rainbow trout; x, Xenopus; z, zebrafish. conclusion is slightly different from the scheme presented by Qiu and Hogstrand (Qiu and Hogstrand, 2004) who proposed that the mammalian TRPV5 and TRPV6 sub-families may have originated from a single ancestral TRPV6 gene. Interestingly, neither the amphibian (Xenopus) nor the avian (Gallus) calcium channels appear to group with either the fish or the mammalian channels.…”

Section: Discussioncontrasting

confidence: 88%

“…The predominance of ECaC mRNA expression in the gill of pufferfish has also been reported (Qiu and Hogstrand, 2004). In contrast to mammals, in which ECaC expression is high in vitamin D 3 -sensitive tissues (Van Ball et al, 1996;Hoenderop et al, 1999;Hoenderop et al, 2000;Wood et al, 2001;den Dekker et al, 2003), ECaC expression was low in rainbow trout intestine and kidney (Fig.·5).…”

Section: Discussionmentioning

confidence: 57%

“…In comparison to the vast body of literature on epithelial Ca 2+ channels in mammals , there is comparatively little known about the nature of the branchial Ca 2+ channel in fish, although it has been cloned from three teleost species: Fugu rubripes (Qiu and Hogstrand, 2004), Danio rerio (Pan et al, 2005; NCBI GenBank accession no. AY325807) and Oncorhynchus mykiss (A. Shahsavarani, B. McNeill, M. Bayaa and S. F. Perry; NCBI GenBank accession no.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterization of a branchial epithelial calcium channel (ECaC) in freshwater rainbow trout (Oncorhynchus mykiss)

Shahsavarani

McNeill

Gálvez

et al. 2006

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussioncontrasting

confidence: 88%

Section: Discussionmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of a branchial epithelial calcium channel (ECaC) in freshwater rainbow trout (Oncorhynchus mykiss)

Shahsavarani

McNeill

Gálvez

et al. 2006

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…In rainbow trout, the branchial ecac responds to variations in external Ca concentrations, and is pivotal in regulating Ca influx via the gills (Shahsavarani & Perry 2006). Qiu & Hogstrand (2004) identified two VDREs in the promoter region of branchial ecac of pufferfish (Fugu rubripes) and later demonstrated an increased expression of ecac in rainbow trout (Oncorhynchus mykiss) gills after i.p. injection of CTR (Qiu et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Retinoic acid cross-talk with calcitriol activity in Atlantic salmon (Salmo salar)

Ørnsrud¹,

Lock²,

Glover³

et al. 2009

Journal of Endocrinology

View full text Add to dashboard Cite

Vitamins A (VA) and D (VD) are metabolised by vertebrates to bioactive retinoic acid (RA) and calcitriol (CTR). RA and CTR involvement in bone metabolism requires finetuned regulation of their synthesis and breakdown. In mammals antagonism of VA and VD is observed, but the mechanism of interaction is unknown. We investigated VA-VD interactions in Atlantic salmon (Salmo salar L.) following i.p. injection of RA and/or CTR. VA metabolites, CTR, calcium (Ca), magnesium (Mg) and phosphorus (P) were determined in plasma. Expression of bone matrix Gla protein (mgp), collagen 1 alpha2 chain (col1a2) and alkaline phosphatase (alp) mRNA was quantified to reflect osteogenesis. Branchial epithelial Ca channel (ecac listed as trpv6 in ZFIN Database) mRNA levels and intestinal Ca and P influx were determined to study Ca/P handling targets of RA and CTR.RA-injection (with or without CTR) decreased plasma CTR-levels three-to sixfold. CTR injection did not affect RA metabolites, but lowered CTR in plasma 3 and 5 days after injection. Lowered plasma CTR correlated with decreased mgp and col1a2 expression in all groups and with decreased alp in CTR-injected fish. RA-treated salmon had enhanced alp expression, irrespective of reduced plasma CTR. Expression of ecac and unidirectional intestinal influx of Ca were stimulated following RA-CTR treatment. Plasma Ca, Mg and P were not affected by any treatment. The results suggest cross-talk of RA with the VD endocrine system in Atlantic salmon. Enhanced Ca flux and osteogenesis (alp transcription) in RA-treated fish and inhibition of mgp expression revealed unprecedented disturbance of Ca physiology in hypervitaminosis A.

show abstract

Toxicological perspective on the osmoregulation and ionoregulation physiology of major ions by freshwater animals: Teleost fish, crustacea, aquatic insects, and Mollusca

Griffith

2016

Enviro Toxic and Chemistry

146

136

View full text Add to dashboard Cite

Anthropogenic sources increase freshwater salinity and produce differences in constituent ions compared with natural waters. Moreover, ions differ in physiological roles and concentrations in intracellular and extracellular fluids. Four freshwater taxa groups are compared, to investigate similarities and differences in ion transport processes and what ion transport mechanisms suggest about the toxicity of these or other ions in freshwater. Although differences exist, many ion transporters are functionally similar and may belong to evolutionarily conserved protein families. For example, the Na+/H+-exchanger in teleost fish differs from the H+/2Na+ (or Ca2+)-exchanger in crustaceans. In osmoregulation, Na+ and Cl− predominate. Stenohaline freshwater animals hyperregulate until they are no longer able to maintain hypertonic extracellular Na+ and Cl− concentrations with increasing salinity and become isotonic. Toxic effects of K+ are related to ionoregulation and volume regulation. The ionic balance between intracellular and extracellular fluids is maintained by Na+/K+-adenosine triphosphatase (ATPase), but details are lacking on apical K+ transporters. Elevated H+ affects the maintenance of internal Na+ by Na+/H+ exchange; elevated HCO3− inhibits Cl− uptake. The uptake of Mg2+ occurs by the gills or intestine, but details are lacking on Mg2+ transporters. In unionid gills, SO42− is actively transported, but most epithelia are generally impermeant to SO42−. Transporters of Ca2+ maintain homeostasis of dissolved Ca2+. More integration of physiology with toxicology is needed to fully understand freshwater ion effects.

show abstract

Functional characterisation and genomic analysis of an epithelial calcium channel (ECaC) from pufferfish, Fugu rubripes

Cited by 78 publications

References 37 publications

Characterization of a branchial epithelial calcium channel (ECaC) in freshwater rainbow trout (Oncorhynchus mykiss)

Characterization of a branchial epithelial calcium channel (ECaC) in freshwater rainbow trout (Oncorhynchus mykiss)

Retinoic acid cross-talk with calcitriol activity in Atlantic salmon (Salmo salar)

Toxicological perspective on the osmoregulation and ionoregulation physiology of major ions by freshwater animals: Teleost fish, crustacea, aquatic insects, and Mollusca

Contact Info

Product

Resources

About