Functional characterisation and transcript analysis of an alkaline phosphatase from the arbuscular mycorrhizal fungus Funneliformis mosseae

Liu, Qianhe; Parsons, A. J.; Xue, Hong; Jones, Chris S.; Rasmussen, Susanne

doi:10.1016/j.fgb.2013.02.009

Cited by 22 publications

(11 citation statements)

References 52 publications

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“…In this study, the decrease in RC-pH represents a strong stress imposed on mycorrhizae, and environmental stresses always inhibit ALP activity (Joner et al, 2000). Similarly, the decrease in HC-pH represented direct stress imposed on external hyphae, and a significant reduction in ALP activity by pH decrease has been reported in Pichia pastoris heterologously expressed with FmALP from F. mosseae (Liu et al, 2013). For polyP accumulation in external hyphae (Supporting Information Fig.…”

Section: Influence Of Medium Ph On Mycorrhizal Colonization and Fungamentioning

confidence: 53%

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External hyphae of Rhizophagus irregularis DAOM 197198 are less sensitive to low pH than roots in arbuscular mycorrhizae: evidence from axenic culture system

Wang

Zhang

Yang

et al. 2017

Environ Microbiol Rep

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The growth of plant roots and arbuscular mycorrhizal fungi (AMF) can be inhibited by low pH; however, it is largely unknown which is more sensitive to low pH. This study aimed to compare the physiological and molecular responses of external hyphae (EH) and roots to low pH in terms of growth, development and functioning. We established AM symbiosis in a two-compartmented system (root compartment, RC; hyphal compartment, HC) using AMF and transformed hairy roots and exposed them to pH 6.5 and/or pH 4.5. The results showed that pH 4.5 significantly decreased root cell viability, while EH at pH 6.5 attenuated the effect. In either RC or HC, pH 4.5 reduced biomass, P content, colonization, ALP activity in roots, and ALP activity and polyphosphate accumulation in EH. GintPT expression in EH was inhibited by pH 4.5 in HC but not in RC. The expression of mycorrhiza-responsive LePTs was significantly reduced by the lower colonization due to decreased pH in either RC or HC, while the expression of non-mycorrhiza-responsive LePTs was not affected. Variation partitioning analysis indicated that EH was less sensitive to low pH than roots. The interactions between roots and EH under low pH stress merit further investigation.

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Section: Influence Of Medium Ph On Mycorrhizal Colonization and Fungamentioning

confidence: 53%

“…Similarly, the decrease in HC‐pH represented direct stress imposed on external hyphae, and a significant reduction in ALP activity by pH decrease has been reported in Pichia pastoris heterologously expressed with FmALP from F . mosseae (Liu et al ., ).…”

Section: Resultsmentioning

confidence: 97%

External hyphae of Rhizophagus irregularis DAOM 197198 are less sensitive to low pH than roots in arbuscular mycorrhizae: evidence from axenic culture system

Wang

Zhang

Yang

et al. 2017

Environ Microbiol Rep

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“…AP activity increased, however, in the rhizosphere of ryegrass inoculated only with F. mosseae. This increase could be explained as follows: (i) the AMF released metabolites that promoted rhizosphere activity (Azcón-Aguilar and Barea 2015), or (ii) the AMF secreted phosphatases that subsequently increased the total pool of these enzymes in the rhizosphere soil (Liu et al 2013;Sato et al 2015). Moreover, F. mosseae inoculation also increased the labile pool of K and the shoot K concentration, as observed previously by Meena et al (2014) and Dominguez-Nunez et al (2016).…”

Section: Impact Of Cadophora Sp and F Mosseae Inoculation On Soil Ementioning

confidence: 56%

Co-inoculation of Lolium perenne with Funneliformis mosseae and the dark septate endophyte Cadophora sp. in a trace element-polluted soil

et al. 2018

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The presence of dark septate endophytes (DSEs) or arbuscular mycorrhizal fungi (AMF) in plant roots and their effects on plant fitness have been extensively described. However, little is known about their interactions when they are simultaneously colonizing a plant root, especially in trace element (TE)-polluted soils. We therefore investigated the effects of Cadophora sp. and Funneliformis mosseae on ryegrass (Lolium perenne) growth and element uptake in a Cd/Zn/Pb-polluted soil. The experiment included four treatments, i.e., inoculation with Cadophora sp., inoculation with F. mosseae, co-inoculation with Cadophora sp. and F. mosseae, and no inoculation. Ryegrass biomass and shoot Na, P, K, and Mg concentrations significantly increased following AMF inoculation as compared to non-inoculated controls. Similarly, DSE inoculation increased shoot Na concentration, whereas dual inoculation significantly decreased shoot Cd concentration. Moreover, oxidative stress determined by ryegrass leaf malondialdehyde concentration was alleviated both in the AMF and dual inoculation treatments. We used quantitative PCR and microscope observations to quantify colonization rates. They demonstrated that DSEs had no effect on AMF colonization, while AMF colonization slightly decreased DSE frequency. We also monitored fluorescein diacetate (FDA) hydrolysis and alkaline phosphatase (AP) activity in the rhizosphere soils. FDA hydrolysis remained unchanged in the three inoculated treatments, but AMF colonization increased AP activity and P mobility in the soil whereas DSE colonization did not alter AP activity. In this experiment, we unveiled the interactions between two ecologically important fungal groups likely to occur in roots which involved a decrease of oxidative stress and Cd accumulation in shoots. These results open promising perspectives on the fungal-based phytomanagement of TE-contaminated sites by the production of uncontaminated and marketable plant biomass.

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“…In the present study, the genomes of most of the Frankia strains were shown to contain an alkaline phosphatase gene (Supplementary Table S2) known to hydrolyze phosphomonoesters and catalyze the transfer of phosphoryl groups to alcohol in the presence of certain phosphate acceptors (Coleman, 1992). It seems likely that this gene is involved in mutualistic relationships between Frankia strains and their host plants by exchanging nutrients in a similar way to that suggested for arbuscular mycorrhizal associations (Aono et al, 2004). In addition, low-affinity inorganic phosphate transporter genes were found in the genomes of the Frankia strains belonging to cluster 1 and F. asymbiotica M16386 T (cluster 4).…”

Section: Resultsmentioning

confidence: 75%

Genomic Insights Into Plant-Growth-Promoting Potentialities of the Genus Frankia

et al. 2019

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This study was designed to determine the plant growth promoting (PGP) potential of members of the genus Frankia . To this end, the genomes of 21 representative strains were examined for genes associated directly or indirectly with plant growth. All of the Frankia genomes contained genes that encoded for products associated with the biosynthesis of auxins [indole-3-glycerol phosphate synthases, anthranilate phosphoribosyltransferases ( trp D), anthranilate synthases, and aminases ( trp A and B)], cytokinins (11 well-conserved genes within the predicted biosynthetic gene cluster), siderophores, and nitrogenases ( nif operon except for atypical Frankia ) as well as genes that modulate the effects of biotic and abiotic environmental stress (e.g., alkyl hydroperoxide reductases, aquaporin Z, heat shock proteins). In contrast, other genes were associated with strains assigned to one or more of four host-specific clusters. The genes encoding for phosphate solubilization (e.g., low-affinity inorganic phosphate transporters) and lytic enzymes (e.g., cellulases) were found in Frankia cluster 1 genomes, while other genes were found only in cluster 3 genomes (e.g., alkaline phosphatases, extracellular endoglucanases, pectate lyases) or cluster 4 and subcluster 1c genomes (e.g., NAD(P) transhydrogenase genes). Genes encoding for chitinases were found only in the genomes of the type strains of Frankia casuarinae , F. inefficax , F. irregularis , and F. saprophytica . In short, these in silico genome analyses provide an insight into the PGP abilities of Frankia strains of known taxonomic provenance. This is the first study designed to establish the underlying genetic basis of cytokinin production in Frankia strains. Also, the discovery of additional genes in the biosynthetic gene cluster involved in cytokinin production opens up the prospect that Frankia may have novel molecular mechanisms for cytokinin biosynthesis.

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Functional characterisation and transcript analysis of an alkaline phosphatase from the arbuscular mycorrhizal fungus Funneliformis mosseae

Cited by 22 publications

References 52 publications

External hyphae of Rhizophagus irregularis DAOM 197198 are less sensitive to low pH than roots in arbuscular mycorrhizae: evidence from axenic culture system

External hyphae of Rhizophagus irregularis DAOM 197198 are less sensitive to low pH than roots in arbuscular mycorrhizae: evidence from axenic culture system

Co-inoculation of Lolium perenne with Funneliformis mosseae and the dark septate endophyte Cadophora sp. in a trace element-polluted soil

Genomic Insights Into Plant-Growth-Promoting Potentialities of the Genus Frankia

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