Functional characterisation of romeharsha and clint1 reaffirms the link between plasma membrane homeostasis, cell size maintenance and tissue homeostasis in developing zebrafish epidermis

Phatak, Mandar; Sonawane, Mahendra

doi:10.1007/s12038-018-9777-y

Cited by 2 publications

(10 citation statements)

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“…In a forward genetic mutagenesis screen aimed at identifying genes important for the maintenance of epidermal integrity and architecture, zebrafish mutants showing transient rounding up of epidermal cells, namely goosepimples ( gsp ) and romeharsha ( rhs ), were isolated [ 46 , 47 ]. Although it is known that the gsp locus encodes for Myosin Vb, an actin based molecular motor [ 45 ], the molecular nature of rhs has remained unknown.…”

Section: Resultsmentioning

confidence: 99%

“…It is an ideal system to investigate mechanisms essential for maintaining epithelial homeostasis. Previously, using two mutant loci, goosepimples ( gsp/myoVb ) and romeharsha (rhs) , a direct link between plasma membrane homeostasis, cell size maintenance, and cell proliferation has been demonstrated [ 45 , 46 ]. In addition, it has been reported that peridermal cells round up in gsp/myoVb and rhs mutant embryos, presumably due to perturbed membrane homeostasis [ 45 , 46 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previously, using two mutant loci, goosepimples ( gsp/myoVb ) and romeharsha (rhs) , a direct link between plasma membrane homeostasis, cell size maintenance, and cell proliferation has been demonstrated [ 45 , 46 ]. In addition, it has been reported that peridermal cells round up in gsp/myoVb and rhs mutant embryos, presumably due to perturbed membrane homeostasis [ 45 , 46 ]. The fate of these rounded up cells and the consequences of their retention or extrusion on tissue architecture have not been investigated so far.…”

Section: Introductionmentioning

confidence: 99%

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Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish

et al. 2021

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Epithelia such as epidermis cover large surfaces and are crucial for survival. Maintenance of tissue homeostasis by balancing cell proliferation, cell size, and cell extrusion ensures epidermal integrity. Although the mechanisms of cell extrusion are better understood, how epithelial cells that round up under developmental or perturbed genetic conditions are reintegrated in the epithelium to maintain homeostasis remains unclear. Here, we performed live imaging in zebrafish embryos to show that epidermal cells that round up due to membrane homeostasis defects in the absence of goosepimples/myosinVb (myoVb) function, are reintegrated into the epithelium. Transcriptome analysis and genetic interaction studies suggest that the transcription factor Grainyhead-like 3 (Grhl3) induces the retention of rounded cells by regulating E-cadherin levels. Moreover, Grhl3 facilitates the survival of MyoVb deficient embryos by regulating cell adhesion, cell retention and epidermal architecture. Our analyses have unraveled a mechanism of retention of rounded cells and its importance in epithelial homeostasis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish

et al. 2021

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“…Several zebrafish epidermis mutants ( goosepimples , romeharsha , clint1 , penner , etc.) were important for the discovery of genes with functions in epidermal development and maintenance 12,13 …”

Section: Introductionmentioning

confidence: 99%

“…were important for the discovery of genes with functions in epidermal development and maintenance. 12,13 In zebrafish, formation of the epidermis initiates at early stages of development, and it remains only two cell layers thick for an extended period of time until additional cell layers are added at post-larval stages. 4 The new suprabasal cell layers appear by a process known as epidermal stratification, which begins between 30-40 days of age, or when post-larval fish reach 5.0-6.0 mm SL (standard length) prior to squamation.…”

Section: Introductionmentioning

confidence: 99%

The dynamics of epidermal stratification during post‐larval development in zebrafish

Rangel‐Huerta

Guzmán

Maldonado

2020

Developmental Dynamics

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Background The epidermis, as a defensive barrier, is a consistent trait throughout animal evolution. During post‐larval development, the zebrafish epidermis thickens by stratification or addition of new cell layers. Epidermal basal stem cells, expressing the transcription factor p63, are known to be involved in this process. Zebrafish post‐larval epidermal stratification is a tractable system to study how stem cells participate in organ growth. Methods We used immunohistochemistry, in combination with EdU cell proliferation detection, to study zebrafish epidermal stratification. For this procedure, we selected a window of post‐larval stages (5‐8 mm of standard length or SL, which normalizes age by size). Simultaneously, we used markers for asymmetric cell division and the Notch signaling pathway. Results We found that epidermal stratification is the consequence of several events, including changes in cell shape, active cell proliferation and asymmetrical cell divisions. We identified a subset of highly proliferative epidermal cells with reduced levels of p63, which differed from the basal stem cells with high levels of p63. Additionally, we described different mechanisms that participate in the stratification process, including the phosphorylation of p63, asymmetric cell division regulated by the Par3 and LGN proteins, and expression of Notch genes.

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Functional characterisation of romeharsha and clint1 reaffirms the link between plasma membrane homeostasis, cell size maintenance and tissue homeostasis in developing zebrafish epidermis

Cited by 2 publications

References 42 publications

Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish

Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish

The dynamics of epidermal stratification during post‐larval development in zebrafish

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