Functional characterization and correlation analysis of phenylalanine ammonia-lyase (PAL) in coumarin biosynthesis from Peucedanum praeruptorum Dunn

Sui, Ziwei; Luo, Jun; Yao, Ruolan; Huang, C.H.; Zhao, Yucheng; Kong, Ling–Yi

doi:10.1016/j.phytochem.2018.11.006

Cited by 30 publications

(14 citation statements)

References 44 publications

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“…Phenylalanine ammonia-lyase ( PAL ), as the first rate-limiting enzyme of the phenylalanine pathway, plays an important role in regulating the synthesis of flavonoids, phenols and coumarin compounds. PpPAL could respond to abiotic stresses such as jasmonic acid, UV-B, and cold treatment, leading to a rapid increase in the expression level in P. praeruptorum ( Sui et al, 2019 ). 4-Coumarate: CoA ligase (4CL) is an important enzyme in the phenylpropanoid-branching pathway, which is responsible for catalyzing the synthesis of cinnamoyl CoA, p-coumaryl CoA, caffeoyl CoA, and ferulic acid CoA.…”

Section: Introductionmentioning

confidence: 99%

De novo Transcriptome Sequencing Coupled With Co-expression Analysis Reveal the Transcriptional Regulation of Key Genes Involved in the Formation of Active Ingredients in Peucedanum praeruptorum Dunn Under Bolting Period

Song

Jia

et al. 2021

Front. Genet.

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Peucedanum praeruptorum Dunn is a perennial and one-off flowering plant of the Peucedanum genus in Umbelliferae. The cultivated P. praeruptorum Dunn usually grows nutritionally in the first year and then moves into the reproductive growth in the second year. The lignification of the roots caused by bolting leads to the quality decline of crude materials. Since most of the previous studies have dealt with coumarin biosynthesis and identification of functional genes in P. praeruptorum, the scientific connotation of the inability that the bolted P. praeruptorum cannot be used medically is still unclear. Here, we employed a transcriptome sequencing combined with coexpression analysis to unearth the regulation mechanism of key genes related to coumarin synthesis in pre- and postbolting period, and to explore the mechanisms underlying the effects of bolting on the formation and transport of coumarins between the annual and biennial plants. Six cDNA libraries were constructed, and the transcripts were sequenced and assembled by Illumina Hiseq platform. A total of 336,505 unigenes were obtained from 824,129 non-redundant spliced transcripts. Unigenes (114,488) were annotated to the NCBI nr database, 119,017 and 10,475 unigenes were aligned to Gene Ontology (GO) functional groups and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, respectively. Differential expression analysis screened out a series of upregulated and downregulated genes related to the phenylpropanoid pathway. The heatmap clustering showed that the similar expression patterns were both observed in groups C vs. D and groups C vs. F. The WGCNA-based coexpression was performed to elucidate the module and trait relationship to unearth important genes related to the bolting process. Seven pivotal modules on the KEGG functional annotations suggested these genes were mainly enriched in the process of plant–pathogen interaction, plant hormone signal transduction, MAPK signaling pathway, α-linolenic acid metabolism, circadian rhythm, and phenylpropanoid pathway. Further analysis provided clues that the key genes of the phenylpropanoid pathway, the ABC transporters, the apoptosis-related and circadian rhythm regulatory genes may play pivotal roles in regulating bolting signaling, biosynthesis, and transportation of coumarins.

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Section: Introductionmentioning

confidence: 99%

De novo Transcriptome Sequencing Coupled With Co-expression Analysis Reveal the Transcriptional Regulation of Key Genes Involved in the Formation of Active Ingredients in Peucedanum praeruptorum Dunn Under Bolting Period

Song

Jia

et al. 2021

Front. Genet.

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“…Coumarins are a major group of natural plant products derived from the phenylpropanoid pathway ( Hawryl, Soczewinski & Dzido, 2000 ; Lin et al, 2013 ), and PAL, BGA, C4H, 4CL and F6′H1 are key enzymes in coumarin biosynthesis ( Poulton, Mcree & Conn, 1980 ; Yang et al, 2015 ). Studies have shown that the expression level of PAL is increased under methyl jasmonate, UV irradiation and cold treatment, resulting in the improvement of coumarin content ( Sui et al, 2019 ). Overexpression of AgC4H or AgPAL genes promotes the production of decursinol angelate (pyranocoumarins) in Angelica gigas ( Park, Park & Park, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome analysis of Cnidium monnieri (L.) Cuss and detection of genes related to coumarin biosynthesis

Shi

Zhang

Peng

et al. 2020

PeerJ

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Cnidium monnieri (L.) Cuss (C. monnieri) is one of the most widely used traditional herbal medicines, exhibiting a wide range of pharmacological functions for treating asynodia, trichomonas vaginitis, and osphyalgia. Its important medicinal value comes from its abundance of coumarins. To identify genes involved in coumarin biosynthesis and accumulation, we analyzed transcriptome data from flower, leaf, root and stem tissues of C. monnieri. A total of 173,938 unigenes with a mean length of 1,272 bp, GC content of 38.79%, and N50 length of 2,121 bp were assembled using the Trinity program. Of these, 119,177 unigenes were annotated in public databases. We identified differentially expressed genes (DEGs) based on expression profile analysis. These DEGs exhibited higher expression levels in flower tissue than in leaf, stem or root tissues. We identified and analyzed numerous genes encoding enzymes involved in coumarin biosynthesis, and verified genes encoding key enzymes using quantitative real-time PCR. Our transcriptome data will make great contributions to research on C. monnieri and provide clues for identifying candidate genes involved in coumarin metabolic pathways.

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“…A total of 433 metabolites were evaluated, including flavonoids (76), lipids (59), amino acids and derivatives (52), phenolic acids (64), alkaloids (39), organic acids (32), terpenoids (10), lignans and coumarins (10) among others (Supplementary Table S2).…”

Section: Metabolites Analysis Of Roots Under Different Led Lightsmentioning

confidence: 99%

“…Coumarins are widely distributed in numerous plant species and are involved in defense against phytopathogens, abiotic stress, oxidative stress, and clinical diseases. Furthermore, coumarins are the primary secondary metabolites of S. glabra [57][58][59]. Even though it is well known that coumarins are produced via the phenylpropanoid pathway, the specific biosynthetic pathways of most coumarins are obscure [57,60].…”

Section: Phenylpropanoid Metabolism Biosynthesismentioning

confidence: 99%

Consequences of LED Lights on Root Morphological Traits and Compounds Accumulation in Sarcandra glabra Seedlings

Xie

Tarin

Chen

et al. 2021

IJMS

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This study evaluated the effects of different light spectra (white light; WL, blue light; BL and red light; RL) on the root morphological traits and metabolites accumulation and biosynthesis in Sarcandra glabra. We performed transcriptomic and metabolomic profiling by RNA-seq and ultra-performance liquid chromatography−electrospray ionization−tandem mass spectrometry (UPLC-ESI-MS/MS), respectively. When morphological features were compared to WL, BL substantially increased under-ground fresh weight, root length, root surface area, and root volume, while RL inhibited these indices. A total of 433 metabolites were identified, of which 40, 18, and 68 compounds differentially accumulated in roots under WL (WG) vs. roots under BL (BG), WG vs. roots under RL (RG), and RG vs. BG, respectively. In addition, the contents of sinapyl alcohol, sinapic acid, fraxetin, and 6-methylcoumarin decreased significantly in BG and RG. In contrast, chlorogenic acid, rosmarinyl glucoside, quercitrin and quercetin were increased considerably in BG. Furthermore, the contents of eight terpenoids compounds significantly reduced in BG. Following transcriptomic profiling, several key genes related to biosynthesis of phenylpropanoid-derived and terpenoids metabolites were differentially expressed, such as caffeic acid 3-O-methyltransferase) (COMT), hydroxycinnamoyl-CoA shikimate hydroxycinnamoyl transferase (HCT), O-methyltransferase (OMT), and 1-deoxy-D-xylulose-5-phosphate synthetase (DXS). In summary, our findings showed that BL was suitable for growth and accumulation of bioactive metabolites in root tissue of S. glabra. Exposure to a higher ratio of BL might have the potential to improve the production and quality of S. glabra seedlings, but this needs to be confirmed further.

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Functional characterization and correlation analysis of phenylalanine ammonia-lyase (PAL) in coumarin biosynthesis from Peucedanum praeruptorum Dunn

Cited by 30 publications

References 44 publications

De novo Transcriptome Sequencing Coupled With Co-expression Analysis Reveal the Transcriptional Regulation of Key Genes Involved in the Formation of Active Ingredients in Peucedanum praeruptorum Dunn Under Bolting Period

De novo Transcriptome Sequencing Coupled With Co-expression Analysis Reveal the Transcriptional Regulation of Key Genes Involved in the Formation of Active Ingredients in Peucedanum praeruptorum Dunn Under Bolting Period

De novo transcriptome analysis of Cnidium monnieri (L.) Cuss and detection of genes related to coumarin biosynthesis

Consequences of LED Lights on Root Morphological Traits and Compounds Accumulation in Sarcandra glabra Seedlings

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