Functional characterization and quantitative expression analysis of two GnRH-related peptide receptors in the mosquito, Aedes aegypti

Oryan, Alireza; Wahedi, Azizia; Paluzzi, Jean‐Paul

doi:10.1016/j.bbrc.2018.02.088

Cited by 33 publications

(44 citation statements)

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“…On the tissue level, highest expression in adults was found in thorax and abdomen with low levels detected in ovaries (Kaufmann et al, 2009). Similar expression profiles were determined recently by Oryan et al (2018) using RT-qPCR: expression in head, thoracic ganglia, accessory reproductive tissues and carcass of adult females as well as in abdominal ganglia and specific enrichment in the carcass and reproductive organs of adult males. Similarly, CRZR was mostly expressed during later development, including pupal and adult stages.…”

Section: Introductionsupporting

confidence: 81%

“…The AKH precursor transcripts are present in head/thorax region of pupae and adult females and in the abdomen of males (Kaufmann et al, 2009). Expression of AKHR was shown in all life stages (Kaufmann et al, 2009), but similar to the ACPR and ACP transcripts, AKHR transcript variants were found to be significantly enriched in early adult stages (Oryan et al, 2018). On the tissue level, highest expression in adults was found in thorax and abdomen with low levels detected in ovaries (Kaufmann et al, 2009).…”

Section: Introductionmentioning

confidence: 94%

“…The presence of mature AKH and corazonin, but not of ACP, was shown by direct mass profiling (Predel et al, 2010). The AKH and ACP precursors have been cloned (Kaufmann et al, 2009) as have one CRZ receptor (CRZR) and various variants of the AKHR and ACPR (Kaufmann et al, 2009; Oryan et al, 2018; Wahedi and Paluzzi, 2018). Each receptor is very selective and responds only to its cognate peptide, thus there are indeed three completely separate and independently working neuroendocrine systems active in this mosquito species.…”

Section: Introductionmentioning

confidence: 99%

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Insight into mosquito GnRH-related neuropeptide receptor specificity revealed through analysis of naturally occurring and synthetic analogs of this neuropeptide family

Wahedi

GÄde

Paluzzi

2019

Preprint

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Section: Introductionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Insight into mosquito GnRH-related neuropeptide receptor specificity revealed through analysis of naturally occurring and synthetic analogs of this neuropeptide family

Wahedi

GÄde

Paluzzi

2019

Preprint

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“…The open reading frame of the cloned A. aegypti CAPAr was inserted into pcDNA3.1+ mammalian expression vector following procedures described previously 58–60 . Using a recombinant CHO-K1 cell line stably expressing aequorin 61 , A. aegypti CAPAr was transiently expressed following growth and transfection conditions as reported recently 58 .…”

Section: Methodsmentioning

confidence: 99%

“…Using a recombinant CHO-K1 cell line stably expressing aequorin 61 , A. aegypti CAPAr was transiently expressed following growth and transfection conditions as reported recently 58 . Cells were harvested for the functional assay at 48 hours post-transfection by detaching cells from the culture flask using 5mM EDTA in Dulbecco’s PBS (DPBS; Wisent Corp., St. Bruno, QC) and later cells were resuspended at a concentration of 10 6 -10 7 cells/mL in assay media and incubated with coelenterazine h as described previously 60 . Prior to running the functional assay, cells were diluted 10-fold in assay media and left to incubate for one additional hour.…”

Section: Methodsmentioning

confidence: 99%

An anti-diuretic hormone receptor in the human disease vector,Aedes aegypti: identification, expression analysis and functional deorphanization

Sajadi

Uyuklu

Lajevardi

et al. 2019

Preprint

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Insect CAPA neuropeptides, which are homologs of mammalian neuromedin U, have been described in various insect species and are known to influence ion and water balance by regulating the activity of the Malpighian ‘renal’ tubules (MTs). A number of diuretic hormones have been shown to increase primary fluid and ion secretion by the insect MTs and, in the adult female mosquito, a calcitonin-related peptide (DH31) also known as mosquito natriuretic peptide, increases sodium secretion at the expense of potassium to remove the excess salt load acquired upon blood-feeding. An endogenous mosquito anti-diuretic hormone was recently described, having inhibitory activity against select diuretic factors and being particularly potent against DH31-stimulated diuresis. In the present study, we have functionally deorphanized, both in vitro and in vivo, a mosquito anti-diuretic hormone receptor (AedaeADHr). Expression analysis by quantitative PCR indicates the receptor is highly enriched in the MTs, and fluorescent in situ hybridization confirms expression within principal cells. Characterization using a heterologous system demonstrated the receptor was highly sensitive to mosquito CAPA peptides. In adult females, AedaeADHr transcript knockdown using RNAi led to the abolishment of CAPA-peptide induced anti-diuretic control of DH31-stimulated MTs. The neuropeptidergic ligand is produced within a pair of neurosecretory cells in each of the six abdominal ganglia, whose axonal projections innervate the abdominal neurohaemal organs (known as the perivisceral organs), where these neurohormones are released into the open circulatory system of the insect. Furthermore, pharmacological inhibition of PKG/NOS signalling abolished the anti-diuretic activity of AedaeCAPA-1, which collectively confirms the role of cGMP/PKG/NOS in this anti-diuretic signalling pathway.SignificanceInsects are by far the most successful and abundant group of organisms on earth. As a result of their small size, insects have a relatively large surface area to volume ratio, raising the potential for rapid gain or loss of water, ions and other molecules including toxins – a phenomenon that applies to insects living in both aquatic and terrestrial environments. In common with many other organisms, hormones are key regulators of the excretory system in insects, and numerous factors control the clearance of excess water and ions (i.e. diuretics) or retention of these elements (i.e. anti-diuretics). Here we characterized an endogenous anti-diuretic hormone receptor in the human disease vector, Aedes aegypti, demonstrating its expression is highly enriched in the Malpighian ‘renal’ tubules and is necessary for eliciting anti-diuretic control of this key component of the mosquito excretory system.

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Functional analysis of adipokinetic hormone signaling in reproductive diapause of Coccinella septempunctata

Shen,

Liu,

Chen

et al. 2024

Pest Management Science

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BACKGROUNDThe ladybeetle, Coccinella septempunctata, an important predator, is widely used to control aphids, whiteflies, mites, thrips, and lepidopteran pests. Diapause control technology is the key to extend C. septempunctata shelf‐life and commercialization. Lipid accumulation is a major feature of reproductive diapause, but the function of AKH signaling as a regulator of lipid mobilization in reproductive diapause remains unclear. This study aimed to identify and characterize AKH and AKHR genes, and clarify their functions in reproductive diapause.RESULTSThe relative expression levels of CsAKH and CsAKHR were the highest in the head and fat body, respectively, and significantly decreased under diapause conditions, both in developmental stages and tissues (head, midgut, fat body, and ovary). Furthermore, CsAKH and CsAKHR expression was increased significantly after JH injection, but CsMet silencing significantly inhibited CsAKH and CsAKHR expression, while CsMet knockdown blocked the induction effect of JH. CsAKH and CsAKHR knockdown significantly reduced water content, increased lipid storage, promoted the expression of genes related to lipid synthesis, meanwhile significantly blocked ovarian development, and induced forkhead box O (FOXO) gene expression in C. septempuctata under reproduction conditions. In contrast, injection of AKH peptide significantly inhibited FOXO expression, reduced lipid storage, and increased water content in C. septempuctata under diapause conditions.CONCLUSIONThese results indicate that CsAKH and CsAKHR are involved in the regulation of lipid accumulation and ovarian development during diapause in C. septempuctata, and provide a promising target for manipulating C. septempunctata diapause.This article is protected by copyright. All rights reserved.

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Functional characterization and quantitative expression analysis of two GnRH-related peptide receptors in the mosquito, Aedes aegypti

Cited by 33 publications

References 53 publications

Insight into mosquito GnRH-related neuropeptide receptor specificity revealed through analysis of naturally occurring and synthetic analogs of this neuropeptide family

Insight into mosquito GnRH-related neuropeptide receptor specificity revealed through analysis of naturally occurring and synthetic analogs of this neuropeptide family

An anti-diuretic hormone receptor in the human disease vector,Aedes aegypti: identification, expression analysis and functional deorphanization

Functional analysis of adipokinetic hormone signaling in reproductive diapause of Coccinella septempunctata

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