Functional characterization of a defense‐responsive bulnesol/elemol synthase from potato

Dwivedi, Varun; Rao, Suchetha; Bomzan, Dikki Pedenla; Kumar, Sudesh; Shanmugam, Pragadheesh V.; Olsson, Shannon B.; Nagegowda, Dinesh A.

doi:10.1111/ppl.13199

Cited by 7 publications

(5 citation statements)

References 48 publications

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“…It encoded a sesquiterpene synthase (StBUS/ELS) forming bulnesol/elemol and in planta results indicated that StBUS/ELS could have a direct role in providing tolerance against bacterial and fungal pathogens in potato (Dwivedi et al., 2021). During the course of our previous work, we identified another TPS belonging to the TPS‐a clade (StTPS18 , accession number MF997578) that showed highly induced expression in response to only bacterial ( P. syringae) but not fungal ( A. solani ) inoculation (Figure S1 and Table S1–S2) (Dwivedi et al., 2021). Reverse transcriptase quantitative polymerase chain reaction (RT‐qPCR) analysis showed that StTPS18 exhibited a similar pattern of induction in response to both avirulent and virulent strains of P. syringae (Figure 1a).…”

Section: Resultsmentioning

confidence: 99%

“…Recently, a TPS that exhibited induced expression in response to methyl jasmonate (MeJA), and bacterial and fungal inoculations was functionally characterized by our group. It encoded a sesquiterpene synthase (StBUS/ELS) forming bulnesol/elemol and in planta results indicated that StBUS/ELS could have a direct role in providing tolerance against bacterial and fungal pathogens in potato (Dwivedi et al., 2021). During the course of our previous work, we identified another TPS belonging to the TPS‐a clade (StTPS18 , accession number MF997578) that showed highly induced expression in response to only bacterial ( P. syringae) but not fungal ( A. solani ) inoculation (Figure S1 and Table S1–S2) (Dwivedi et al., 2021).…”

Section: Resultsmentioning

confidence: 99%

“…In order to identify putative TPS genes in potato, searches in the PGSC database were performed using “terpene synthase” as a query. In addition, the database was further screened by BLASTp analysis using characterized TPS sequences (Dwivedi et al., 2021). Multiple protein sequence alignment was performed using ClustalW and a maximum‐likelihood tree was constructed using MEGA 6.0 (Tamura et al., 2013) with the Jones‐Taylor‐Thornton (JTT) model.…”

Section: Methodsmentioning

confidence: 99%

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An inducible potato (E,E)‐farnesol synthase confers tolerance against bacterial pathogens in potato and tobacco

et al. 2022

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SUMMARY Terpene synthases (TPSs) have diverse biological functions in plants. Though the roles of TPSs in herbivore defense are well established in many plant species, their role in bacterial defense has been scarce and is emerging. Through functional genomics, here we report the in planta role of potato (Solanum tuberosum) terpene synthase (StTPS18) in bacterial defense. Expression of StTPS18 was highest in leaves and was induced in response to Pseudomonas syringae and methyl jasmonate treatments. The recombinant StTPS18 exhibited bona fide (E,E)‐farnesol synthase activity forming a sesquiterpenoid, (E,E)‐farnesol as the sole product, utilising (E,E)‐farnesyl diphosphate (FPP). Subcellular localization of GFP fusion protein revealed that StTPS18 is localized to the cytosol. Silencing and overexpression of StTPS18 in potato resulted in reduced and enhanced tolerance, respectively, to bacterial pathogens P. syringae and Ralstonia solanacearum. Bacterial growth assay using medium containing (E,E)‐farnesol significantly inhibited P. syringae growth. Moreover, StTPS18 overexpressing transgenic potato and Nicotiana tabacum leaves, and (E,E)‐farnesol and P. syringae infiltrated potato leaves exhibited elevated expression of sterol pathway and members of pathogenesis‐related genes with enhanced phytosterol accumulation. Interestingly, enhanced phytosterols in 13C3‐(E,E)‐farnesol infiltrated potato leaves were devoid of any noticeable 13C labeling, indicating no direct utilization of (E,E)‐farnesol in phytosterols formation. Furthermore, leaves of StTPS18 overexpressing transgenic lines had no detectable (E,E)‐farnesol similar to the control plant, and emitted lower levels of sesquiterpenes than the control. These findings point towards an indirect involvement of StTPS18 and its product (E,E)‐farnesol in bacterial defense through upregulation of phytosterol biosynthesis and defense genes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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An inducible potato (E,E)‐farnesol synthase confers tolerance against bacterial pathogens in potato and tobacco

et al. 2022

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“…The oven temperature was increased at the rate of 3 °C min from 60 °C to 150 °C (ramp-1) with a 10 min isothermal hold at 150 °C, then increased to 300 °C at the rate of 10 °C per min (ramp-2). To analyse at a lower temperature sample was injected into GC at 150 °C inlet temperature with the same parameters (Dwivedi et al 2021). Same samples were analysed in GC-MS with the parameter as described earlier.…”

Section: Methodsmentioning

confidence: 99%

Analysis of root volatiles and functional characterization of a root-specific germacrene A synthase inArtemisia pallens

Kiran,

Narayanan,

Mohapatra

et al. 2023

Preprint

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Davana (Artemisia pallens) is a valuable aromatic herb within the Asteraceae family, highly prized for its essential oil (EO) produced in the aerial parts. However, the root volatiles and their specific composition, and genes responsible for root volatiles have remained unexplored until now. Here, we show thatA. pallensroots possess distinct oil bodies and yields ∼0.05% of EO, which is primarily composed of sesquiterpenes β-elemene, neryl isovalerate, β-selinene, and α-selinene, and trace amounts of monoterpenes β-myrcene, D-limonene. This shows that, besides aerial parts, roots of davana can also be a source of unique EO. Moreover, we functionally characterized a terpene synthase (ApTPS1) that exhibited highin silicoexpression in the root transcriptome. The recombinant ApTPS1 showed the formation of β-elemene and germacrene A withE,E-farnesyl diphosphate (FPP) as a substrate. Further, detailed analysis of assay products revealed that β-elemene was the thermal rearrangement product of germacrene A. Furthermore, the functional expression of ApTPS1 inSaccharomyces cerevisiaeconfirmed thein vivoβ-elemene/germacrene A synthase activity of ApTPS1. At the transcript level,ApTPS1displayed predominant expression in root, with significantly lower level of expression in other tissues. This expression pattern ofApTPS1positively correlated with the tissue-specific accumulation level of β-elemene. Overall, these findings provide fundamental insights into the EO profile of davana roots, and the contribution of ApTPS1 in the formation of a major root volatile.Main conclusionThe study demonstrated thatArtemisia pallensroots can be a source of terpene-rich essential oil and root-specific ApTPS1 forms germacrene-A contributing to major root volatiles.

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“…Sesquiterpenes are mainly synthesized by the mevalonate (MVA) pathway in plants [29]. The universal precursor, farnesyl diphosphate (FPP), can be converted into different sesquiterpene skeletons by different sesquiterpene synthases (SSs) [30]. Previous studies showed that drought stress changed the content of sesquiterpenes in Ocimum basilicum L. by regulating the expression of key genes encoding enzymes of terpene biosynthesis [31].…”

Section: Introductionmentioning

confidence: 99%

Effect of drought on photosynthesis, total antioxidant capacity, bioactive component accumulation, and the transcriptome of Atractylodes lancea

Zhang

Liu

et al. 2021

BMC Plant Biol

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Background Atractylodes lancea (Thunb.) DC, a medicinal herb belonging to the Asteraceae family, often faces severe drought stress during its growth. Until now, there has been no research on the effect of drought stress on the quality formation of A. lancea. Therefore, the present study aimed to study the effects of drought stress on A. lancea through physical and chemical analysis, and to reveal the related molecular mechanisms via transcriptome analysis. Results The photosynthesis was markedly inhibited under drought stress. There were alterations to photosynthetic parameters (Pn, Gs, Ci) and chlorophyll fluorescence (Fv/Fm, NPQ), and the chlorophyll content decreased. Twenty genes encoding important regulatory enzymes in light and dark reactions, including the Rubisco gene of the Calvin cycle, were significantly downregulated. After exposure to drought stress for more than 4 days, the activities of four antioxidative enzymes (SOD, POD CAT and APX) began to decrease and continued to decrease with longer stress exposure. Meanwhile, most of the genes encoding antioxidative enzymes were downregulated significantly. The downregulation of 21 genes related to the respiratory electron transport chain indicated that the blocked electron transfer accelerated excessive ROS. The MDA content was significantly elevated. The above data showed that 15 days of drought stress caused serious oxidative damage to A. lancea. Drought stress not only reduced the size and dry weight of A. lancea, but also lowered the amount of total volatile oil and the content of the main bioactive components. The total volatile oil and atractylodin content decreased slightly, whereas the content of atractylon and β-eudesmol decreased significantly. Moreover, ten significantly downregulated genes encoding sesquiterpene synthase were mainly expressed in rhizomes. Conclusions After exposed to drought stress, the process of assimilation was affected by the destruction of photosynthesis; stress tolerance was impaired because of the inhibition of the antioxidative enzyme system; and bioactive component biosynthesis was hindered by the downregulation of sesquiterpene synthase-related gene expression. All these had negative impacts on the quality formation of A. lancea under drought stress.

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Functional characterization of a defense‐responsive bulnesol/elemol synthase from potato

Cited by 7 publications

References 48 publications

An inducible potato (E,E)‐farnesol synthase confers tolerance against bacterial pathogens in potato and tobacco

An inducible potato (E,E)‐farnesol synthase confers tolerance against bacterial pathogens in potato and tobacco

Analysis of root volatiles and functional characterization of a root-specific germacrene A synthase inArtemisia pallens

Effect of drought on photosynthesis, total antioxidant capacity, bioactive component accumulation, and the transcriptome of Atractylodes lancea

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