Functional characterization of a novel tumor necrosis factor gene (TNF-New) in rock bream (Oplegnathus fasciatus)

Ko, Sungjae; Lim, Jongwon

doi:10.1016/j.dci.2021.104269

Cited by 2 publications

(3 citation statements)

References 42 publications

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“…In the present study, ToTNFα transcripts were constitutively expressed in all tested tissues of healthy fish, which was similar to the expression of TNFα in other teleosts (Hirono et al, 2000;García-Castillo et al, 2002;Zou et al, 2003;Savan and Sakai, 2004;Hong et al, 2013;Biswas et al, 2015;Cui et al, 2020;Huang et al, 2020;Kong et al, 2021;Ko et al, 2022). The mRNA level of ToTNFα was rich in the head-kidney, gill, skin and small intestine and poor in the stomach, liver and blood.…”

Section: Discussionsupporting

confidence: 85%

“…In fish, TNFα has been found in more than 20 species, such as in important seawater species, Japanese flounder (Paralichthys olivaceus) (Hirono et al, 2000), gilthead seabream (Sparus aurata) (García-Castillo et al, 2002), rainbow trout (Oncorhynchus mykiss) (Zou et al, 2002;Hong et al, 2013), catfish (Ictalurus punctatus) (Zou et al, 2003), turbot (Psetta maxima) (Ordás et al, 2007), sea bass (Dicentrarchus labrax) (Nascimento et al, 2007), bluefin tuna (Thunnus orientalis) (Kadowaki et al, 2009), striped trumpeter (Latris lineata) (Covello et al, 2009), orange-spotted grouper (Epinephelus coioides) (Lam et al, 2011), rock bream (Oplegnathus fasciatus) (Kim et al, 2011;Ko et al, 2022), fugu (Fugu rubripes) (Biswas et al, 2015), large yellow croaker (Larimichthys crocea) (Huang et al, 2020), and pufferfish (Takifugu obscurus) (Kong et al, 2021), and in important freshwater species, common carp (Cyprinus carpio) (Forlenza et al, 2009;Zhao et al, 2012), zebrafish (Danio rerio) (Savan et al, 2005), ayu (Plecoglossus altivelis) (Uenobe et al, 2007), goldfish (Carassius auratus) (Grayfer et al, 2008), grass carp (Ctenopharyngodon idella) (Zhang et al, 2012), and snakehead (Channa argus) (Cui et al, 2020). Furthermore, two TNFα isoforms have been characterized in Atlantic bluefin tuna Thunnus thynnus (Lepen Plei&cacute;et al, 2014), T. orientalis (Kadowaki et al, 2009), E. coioides (Lam et al, 2011), and C. auratus (Grayfer et al, 2008), three TNFα isoforms in O. mykiss (Hong et al, 2013), and even four in C. carpio (Savan and Sakai, 2004;Zhao et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Nuclear Factor-κB Plays a Positive Role in TNFα Expression in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

Zhu

Guo

et al. 2022

Front. Mar. Sci.

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Tumour necrosis factor-α (TNFα) is a multifarious mediator of lymphoid tissue growth and antimicrobial defence mechanisms, and it acts as a pro-inflammatory regulator. The function of TNFα in parasite infection and the underlying mechanism through which nuclear factor-κB (NF-κB) regulates TNFα remain largely unclear in teleosts. In the present study, TNFα (ToTNFα) from golden pompano (Trachinotus ovatus) was identified, and its sequence features and expression levels were determined. The genomic DNA sequence is composed of 1,130 bp, consists of four exons and three introns, and encodes 341 amino acid polypeptides. The putative protein sequence shares 34.7%–61.9% identity with fish TNFα and possesses a TNF family signature and two conserved cysteine residues. Moreover, the expressions of ToNF-κB and ToTNFα are constitutively expressed in all examined tissues, with higher levels observed in the immune relevant tissues. Both ToNF-κB and ToTNFα transcription was increased in the local infection sites (skin and gill) and system immune tissues (liver, spleen and head kidney) after Cryptocaryon irritans stimulation. In addition, to investigate whether ToNF-κB is a regulator of ToTNFα, promoter analysis was performed. The region from -970 to +79 bp is known as the core promoter by different truncated mutants of ToTNFα. Subsequently, the activity of the ToTNFα-p2 promoter was dramatically reduced after targeted mutation of the M6-binding site. Additionally, an electrophoretic mobile shift assay (EMSA) verified that ToNF-κB interacted with the M6-binding site in the ToTNFα promoter region to control the expression of ToTNFα. In conclusion, the present study provides the positive regulation of TNFα transcription by NF-κB and contributes to a better understanding of the transcriptional mechanism of TNFα in fish.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Nuclear Factor-κB Plays a Positive Role in TNFα Expression in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

Zhu

Guo

et al. 2022

Front. Mar. Sci.

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“…Q-PCR was performed in a 20 μL reaction containing 10 μL of SYBR Green Real-time PCR Master Mix (Takara, Japan), 0.4 mM of each forward- and reverse- primer, and 4 μL of cDNA according to the following protocol: 60 s at 95 °C; the template was amplified for 40 cycles of denaturation for 15 s at 95 °C, annealing, and extension for 1 min at 60 °C using the LC96 real-time thermocycler (Roche) [ 21 ]. Each sample was analyzed in duplicate combined with a serial dilution of references for the absolute quantification analysis, and the transcript level was calculated using the integrated software previously described [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Pathogenic Mechanism of a Highly Virulent Infectious Hematopoietic Necrosis Virus in Head Kidney of Rainbow Trout (Oncorhynchus mykiss) Analyzed by RNA-Seq Transcriptome Profiling

Kim

Cho

Lim

et al. 2022

Viruses

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Infectious hematopoietic necrosis virus (IHNV) is a pathogen that causes high rates of mortality in salmonid fishes. Therefore, an RNA-seq-based transcriptome analysis was performed in the head kidney of rainbow trout infected with a highly virulent IHNV strain to understand the pathogenesis of and defense strategies for IHNV infection in rainbow trout. The results showed that the numbers of DEGs were 618, 2626, and 774 (control vs. IHNV) on days 1, 3, and 5, respectively. Furthermore, the enrichment analysis of gene ontology (GO) annotations to classify DEGs showed that GO terms considerably associated with DEGs were gluconeogenesis, inflammatory response, and cell adhesion in the Biological Process (BP) category, apical plasma membrane, extracellular matrix (ECM) in the Cellular Component category, and transporter activity, integrin binding, and protein homodimerization activity in the Molecular Function category, on days 1, 3, and 5, respectively. Notably, GO terms in the BP category, including the negative regulation of type I interferon production and positive regulation of interleukin-1β secretion, were commonly identified at all time points. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, complement and coagulation cascades were commonly identified at all time points. Importantly, the widely recognized GO terms and KEGG pathways extensively linked to DEGs were related to energy metabolism on day 1, the immune response on day 3, and cell proliferation on day 5. Furthermore, protein–protein interaction networks and centrality analysis showed that the metabolism and signaling transduction pathways were majorly upregulated. Conclusively, the virulent IHNV infection drives pathogenesis by activating the metabolic energy pathway for energy use for viral replication, facilitating necrosis through autophagy, and causing a shutoff response of the host immune system through the downregulation of type I IFN at the initial stage of infection.

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Functional characterization of a novel tumor necrosis factor gene (TNF-New) in rock bream (Oplegnathus fasciatus)

Cited by 2 publications

References 42 publications

Nuclear Factor-κB Plays a Positive Role in TNFα Expression in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

Nuclear Factor-κB Plays a Positive Role in TNFα Expression in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

Pathogenic Mechanism of a Highly Virulent Infectious Hematopoietic Necrosis Virus in Head Kidney of Rainbow Trout (Oncorhynchus mykiss) Analyzed by RNA-Seq Transcriptome Profiling

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