Functional characterization of a novel thermophilic exo-arabinanase from Thermothielavioides terrestris

Hemicellulose content affects the properties of cellulose nanofibrils produced from softwood pulp fibres by LPMO

“…In addition, a moderate increase in the initial activity (24%) was also observed after 6 to 24 h of incubation at 40 °C. It is not surprising that TtLPMO9E demonstrated good thermostability, as T. terrestris has proven to be an excellent source of thermostable enzymes, 37,38 and LPMOs in general have been found to be thermally resistant. 39 A model cellulosic substrate, microcrystalline cellulose (MCC) was used to probe the optimal reaction pH.…”

Arabinan saccharification by biogas reactor metagenome-derived arabinosyl hydrolases

“…Usually, the enzymatic synergism of different carbohydrate-active enzymes (CAZymes) plays an important role in deconstructing plant cell wall biomass [ 12 ]. One example is the hydrolysis of arabinans which requires the synergistic action of different arabinosyl hydrolases [ 13 ]. Endo-α- l -1,5-arabinanases which are so far only found in family GH43 randomly cleave the arabinan backbone.…”

“…Endo-α- l -1,5-arabinanases which are so far only found in family GH43 randomly cleave the arabinan backbone. Exo-α- l -arabinofuranosidases, which are encountered in the families GH2, GH3, GH5, GH39, GH43, GH51, GH54, GH62 and are active on 1,2-,1,3-arabinosyl side-chain substitutions or terminal 1,5-linked backbone residues [ 11 , 13 ]. Besides, exo-α- l -1,5-arabinanase activity has been described mainly in family GH93 and also in some representatives from family GH43 which releases arabinose, arabinobiose or arabinotriose as a major products from linear arabinan [ 14 – 17 ].…”

Novel Aspergillus fumigatus recombinant LPMOs: biochemical characteristics, and their participation in saccharification and photobiocatalysis processes

“…For A. nidulans A773 the medium was supplemented with pyridoxine, uracil, and uridine, as previously described (Segato et al, 2012). Escherichia coli Turbo strain (New England BioLabs, Ipswich, MA, USA), used for plasmid replication was cultivated in 50 ml centrifuge tube containing 10 ml of Lysogeny Broth (LB) medium added by ampicillin (100 µg/ml) at 37 C and 180 rpm for 16 h. For heterologous protein expression, the recombinant strain was cultivated in liquid minimal medium supplemented with 3% (w/v) maltose and pyridoxine (1 mg/L) for 2 days at 37 C without shaking (Velasco et al, 2020).…”

“…(5'-GTCCCGTGCCGGTTACTAAGCAGAGAGATCACGAGCGTGCACCTT-3'), containing specific regions (in bold) to allow the gene insertion into pEXPYR expression vector by Gibson Assembly ® method as previously described (Gibson et al, 2009;Velasco et al, 2019Velasco et al, , 2020. The resulting reaction was used to transform Mg + competent E. coli Turbo ® cells (New England BioLabs) by heat-shock and the resulting ampicillin-resistant colonies were randomly screened by colony PCR technique.…”