Functional characterization of ABC transporters mediates multiple neonicotinoid resistance in a field population of Aphis gossypii Glover

Lv, Yuntong; Li, Jianyi; Yan, Kunpeng; Ding, Yaping; Gao, Xiwu; Bi, Rui; Zhang, Hang; Pan, Yiou; Shang, Qingli

doi:10.1016/j.pestbp.2022.105264

Cited by 8 publications

(3 citation statements)

References 39 publications

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“…47 In Italy a population with reduced sensibility to this compound was reported in 2001, but it regained susceptibility after 6 weeks of rearing without selection pressure. 28 Resistance to neonicotinoids may involve different metabolic mechanisms, [48][49][50][51] but also changes in the nicotinic acetylcholine receptor gene sequence, such as that producing the R81T substitution, that are known to confer high degrees of resistance. This mutation is currently found only in two aphid species, A. gossypii and Myzus persicae, and is linked to high fitness costs that can limit its spread across wild individuals.…”

Section: Discussionmentioning

confidence: 99%

Survey of target site mutations linked with insecticide resistance in Italian populations of Aphis gossypii

Cominelli,

Chiesa,

Panini

et al. 2024

Pest Management Science

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BACKGROUNDAphis gossypii is a worldwide agricultural pest that cause high levels of economic losses by feeding and transmitting virus diseases. It is usually controlled by chemical insecticides, but this could lead to the selection of resistant populations. Several SNPs have been identified associated with insecticide resistance. Monitoring activities to detect the presence of such mutations in field populations can have an important role in insect pest management but, currently, no information on Italian strains is available.RESULTSThe presence of target site mutations conferring resistance to different insecticides was analysed in Italian field collected populations of A. gossypii with an allele specific approach (QSGG, Qualitative Sybr‐Green Genotyping). Primers were designed to detect mutations in genes coding acetylcholinesterase (S431F), nicotinic acetylcholine receptor (R81T) and voltage gated sodium channel (M918L and L1014F). S431F was widespread but with high variability across populations. R81T was detected for the first time in Italy but only in 2 populations. The L1014F mutation (kdr) was not found, while in the samples showing the M918L two different nucleotidic substitutions were detected. Mutant allele frequencies were respectively 0.70 (S431), 0.31 (M918) and 0.02 (R81). Further analysis on the voltage gated sodium channel gene showed the presence of eight haplotypes and one nonsynonymous mutation in the gene coding region.CONCLUSIONMultiple target‐site mutations were detected within Italian populations. The combinations of genotypes observed in certain locations could affect negatively the control of this pest. Preliminary insights on the genetic structure in the Italian populations of A. gossypii were acquired.

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Section: Discussionmentioning

confidence: 99%

Survey of target site mutations linked with insecticide resistance in Italian populations of Aphis gossypii

Cominelli,

Chiesa,

Panini

et al. 2024

Pest Management Science

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“…The qRT-PCR program was set as follows: 95 °C for 30 s followed by 40 cycles of 95 °C for 5 s and 60 °C for 34 s. The elongation factor 1alpha (EF1α) gene of A. gossypii was used as a reference gene according to a previous study. 29 Each treatment was performed three times, and the relative expression levels of UGTs were calculated by the 2 −ΔΔCt method. 30 Identification of Candidate UGTs and Phylogenetic Analysis.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…gossypii play important roles in neonicotinoid resistance, specific primers were used to amplify the fragments of selected UGTs and enhanced cyan fluorescence protein ( ECFP ) to synthesize dsRNA by using the T7 RiboMAX Express RNAi System (Promega, America) (Table S2). The RNAi experiment was performed according to a previous study: a 15% source solution containing ECFP dsRNA (as a control) or each UGT dsRNA at a final concentration of 150 ng μL –1 was sealed between two thin pieces of Parafilm in a feeding arena . At least 80 adult aphids (one replicate) were transferred to each arena, and each treatment included three replicates.…”

Section: Methodsmentioning

confidence: 99%

UDP-Glycosyltransferases UGT350C3 and UGT344L7 Confer Tolerance to Neonicotinoids in Field Populations of Aphis gossypii

Li,

Yan,

Kong

et al. 2024

J. Agric. Food Chem.

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Characterization of ATP‐binding cassette transporters associated with emamectin benzoate tolerance: from the model insect Drosophila melanogaster to the agricultural pest Spodoptera frugiperda

Wang,

Guo,

Wen

et al. 2024

Pest Management Science

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BACKGROUNDMultiple families of detoxification genes, including the increasingly recognized family of ATP‐binding cassette (ABC) transporters, work together to influence the toxicity of synthetic insecticides and thus their resistance. Effective management of insecticide resistance requires identification of all toxicity‐affecting members from each family of toxicity‐related genes.RESULTSHere, we used emamectin benzoate (EB), ABC transporters and Spodoptera frugiperda as a working case to test whether the strategy of ‘from the model insect Drosophila melanogaster to agricultural pests’ can identify all or most ABC transporter members related to EB tolerance in S. frugiperda. After confirming the involvement of ABC transporters in the toxicity of EB against fruit fly with the ABC inhibitor verapamil, four ABC transporter genes (DmCG3327, DmCG11147, DmCG4822, and DmCG7627) were found to be involved in EB tolerance using RNA interference‐based family‐wide functional screening. A combination of phylogenic analysis and a reciprocal TBLASTN search identified five S. frugiperda ABC transporter members as homologs (SfABCC4, SfABCG1, and SfABCG23) or one‐way best hits (SfABCG4 and SfABCG20) of the four fly ABC genes. Real‐time quantitative polymerase chain reaction (qPCR) analysis found that all five S. frugiperda ABC transporter genes were inducible by EB, and expressed in all the developmental stages and larval tissues, but with significant quantitative differences among stages and tissues. A cytotoxicity assay of ABC‐overexpressing Sf9 cell lines showed that all the five S. frugiperda ABC transporter genes made Sf9 cells tolerant to EB.CONCLUSIONSThis study not only identifies nine ABC transporter genes related to EB tolerance from D. melanogaster (four genes) and S. frugiperda (five genes), but also demonstrates the utility and effectiveness of the ‘model to pests’ strategy to identify most toxicity‐affecting members from a given family of toxicity‐related genes. © 2024 Society of Chemical Industry.

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Functional characterization of ABC transporters mediates multiple neonicotinoid resistance in a field population of Aphis gossypii Glover

Cited by 8 publications

References 39 publications

Survey of target site mutations linked with insecticide resistance in Italian populations of Aphis gossypii

Survey of target site mutations linked with insecticide resistance in Italian populations of Aphis gossypii

UDP-Glycosyltransferases UGT350C3 and UGT344L7 Confer Tolerance to Neonicotinoids in Field Populations of Aphis gossypii

Characterization of ATP‐binding cassette transporters associated with emamectin benzoate tolerance: from the model insect Drosophila melanogaster to the agricultural pest Spodoptera frugiperda

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