Functional characterization of arginine metabolic pathway enzymes in the antibacterial immune response of penaeid shrimp

Huang, Zishu; Zhang, Yueling; Zheng, Xiaoyu; Liu, Zhuoyan; Yao, Defu; Zhao, Yang; Chen, Xiaohan; Aweya, Jude Juventus

doi:10.1016/j.dci.2021.104293

Cited by 4 publications

(3 citation statements)

References 60 publications

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“…These results indicate that the two nanosystems contribute to transforming from the pro-inflammatory stage to the anti-inflammatory stage of infected cells to repair cell damage. It has been reported that NO has a biphasic effect on immune factors, promoting the expression of iNOS at low concentrations to produce more NO for antibacterial effects, and promoting the production of anti-inflammatory factors at high concentrations to prevent excessive NO from promoting cell repair ( 21 , 53 – 54 ). As reported, irradiation-promoted Arg-PCN@Gel and Arg-PCN produce NO, which induced iNOS expression and further used Arg to produce NO.…”

Section: Resultsmentioning

confidence: 99%

Targeting and arginine-driven synergizing photodynamic therapy with nutritional immunotherapy nanosystems for combating MRSA biofilms

Zhang

Chen

et al. 2023

Sci. Adv.

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The resistance and immune escape of methicillin-resistant Staphylococcus aureus (MRSA) biofilms cause recalcitrant infections. Here, we design a targeting and synergizing cascade PDT with nutritional immunotherapy nanosystems (Arg-PCN@Gel) containing PCN-224 as PDT platform for providing reactive oxygen species (ROS), incorporating arginine (Arg) as nitric oxide (NO) donor to cascade with ROS to produce more lethal ONOO − and promote immune response, and coating with gelatin as targeting agent and persistent Arg provider. The nanosystems adhered to the autolysin of MRSA and inhibited Arg metabolism by down-regulating icdA and icaA . It suppressed polysaccharide intercellular adhesin and extracellular DNA synthesis to prevent biofilm formation. The NO broke mature biofilms and helped ROS and ONOO − penetrate into biofilms to inactivate internal MRSA. Arg-PCN@Gel drove Arg to enhance immunity via inducible NO synthase/NO axis and arginase/polyamine axis and achieve efficient target treatment in MRSA biofilm infections. The targeting and cascading PDT synergized with nutritional immunotherapy provide an effective promising strategy for biofilm-associated infections.

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Section: Resultsmentioning

confidence: 99%

Targeting and arginine-driven synergizing photodynamic therapy with nutritional immunotherapy nanosystems for combating MRSA biofilms

Zhang

Chen

et al. 2023

Sci. Adv.

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“…Moreover, most of these genes were enriched in metabolism pathways, especially, global and overview maps, carbohydrate, amino acid, and lipid metabolism. Given the importance of amino acids and their metabolites in the immune response and immune-related signaling pathways in crustaceans [ 33 , 73 ], we decided to focus on amino acid metabolism in the rest of the study. Thus, further analysis of the 48 genes enriched in amino acid metabolism (Fig.…”

Section: Resultsmentioning

confidence: 99%

Taurine metabolism is modulated in Vibrio-infected Penaeus vannamei to shape shrimp antibacterial response and survival

et al. 2022

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Background Numerous microorganisms are found in aquaculture ponds, including several pathogenic bacteria. Infection of cultured animals by these pathogens results in diseases and metabolic dysregulation. However, changes in the metabolic profiles that occur at different infection stages in the same ponds and how these metabolic changes can be modulated by exogenous metabolites in Penaeus vannamei remain unknown. Results Here, we collected gastrointestinal tract (GIT) samples from healthy, diseased, and moribund P. vannamei in the same aquaculture pond for histological, metabolic, and transcriptome profiling. We found that diseased and moribund shrimp with empty GITs and atrophied hepatopancreas were mainly infected with Vibrio parahaemolyticus and Vibrio harveyi. Although significant dysregulation of crucial metabolites and their enzymes were observed in diseased and moribund shrimps, diseased shrimp expressed high levels of taurine and taurine metabolism-related enzymes, while moribund shrimp expressed high levels of hypoxanthine and related metabolism enzymes. Moreover, a strong negative correlation was observed between taurine levels and the relative abundance of V. parahaemolyticus and V. harveyi. Besides, exogenous taurine enhanced shrimp survival against V. parahaemolyticus challenge by increasing the expression of key taurine metabolism enzymes, mainly, cysteine dioxygenase (CDO) and cysteine sulfinic acid decarboxylase (CSD). Conclusions Our study revealed that taurine metabolism could be modulated by exogenous supplementation to improve crustacean immune response against pathogenic microbes.

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“…The regulation of metabolic pathways for alanine, glutamine, glutamate, arginine, and proline is facilitated through the participation of l -Aspartic acid 61 , 62 . Arginine participates in hormone secretion and immune regulation 63 . Glutamine is a non-essential amino acid involved in regulating shrimp immunity 64 .…”

Section: Discussionmentioning

confidence: 99%

Biochemical indicators, cell apoptosis, and metabolomic analyses of the low-temperature stress response and cold tolerance mechanisms in Litopenaeus vannamei

Zhu,

Li,

Peng

et al. 2024

Sci Rep

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The cold tolerance of Litopenaeus vannamei is important for breeding in specific areas. To explore the cold tolerance mechanism of L. vannamei, this study analyzed biochemical indicators, cell apoptosis, and metabolomic responses in cold-tolerant (Lv-T) and common (Lv-C) L. vannamei under low-temperature stress (18 °C and 10 °C). TUNEL analysis showed a significant increase in apoptosis of hepatopancreatic duct cells in L. vannamei under low-temperature stress. Biochemical analysis showed that Lv-T had significantly increased levels of superoxide dismutase (SOD) and triglycerides (TG), while alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH-L), and uric acid (UA) levels were significantly decreased compared to Lv-C (p < 0.05). Metabolomic analysis displayed significant increases in metabolites such as LysoPC (P-16:0), 11beta-Hydroxy-3,20-dioxopregn-4-en-21-oic acid, and Pirbuterol, while metabolites such as 4-Hydroxystachydrine, Oxolan-3-one, and 3-Methyldioxyindole were significantly decreased in Lv-T compared to Lv-C. The differentially regulated metabolites were mainly enriched in pathways such as Protein digestion and absorption, Central carbon metabolism in cancer and ABC transporters. Our study indicate that low temperature induces damage to the hepatopancreatic duct of shrimp, thereby affecting its metabolic function. The cold resistance mechanism of Lv-T L. vannamei may be due to the enhancement of antioxidant enzymes and lipid metabolism.

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Functional characterization of arginine metabolic pathway enzymes in the antibacterial immune response of penaeid shrimp

Cited by 4 publications

References 60 publications

Targeting and arginine-driven synergizing photodynamic therapy with nutritional immunotherapy nanosystems for combating MRSA biofilms

Targeting and arginine-driven synergizing photodynamic therapy with nutritional immunotherapy nanosystems for combating MRSA biofilms

Taurine metabolism is modulated in Vibrio-infected Penaeus vannamei to shape shrimp antibacterial response and survival

Biochemical indicators, cell apoptosis, and metabolomic analyses of the low-temperature stress response and cold tolerance mechanisms in Litopenaeus vannamei

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