Functional characterization of chitinase-3 reveals involvement of chitinases in early embryo immunity in zebrafish

Teng, Zinan; Sun, Chen; Liu, Shousheng; Wang, Hongmiao; Zhang, Shicui

doi:10.1016/j.dci.2014.06.008

Cited by 23 publications

(14 citation statements)

References 45 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the majority of studies on chitinase have been conducted on microorganisms [12], chitinase is also found and has been investigated in many other biological species, including mammals [13]- [15], fish [16]- [19], mollusks [20] [21], insects [22]- [24], plants [22] [25], and fungi [1] [22]. Since the physiological role of chitinase varies in these organisms, the mechanism and efficiency of chitinase degradation and the substrate specificity have been reported to vary.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Chitinolytic Enzymes in the Organs and cDNA Cloning of Chitinase Isozymes from the Stomach of Two Species of Fish, Chub Mackerel (Scomber japonicus) and Silver Croaker (Pennahia argentata)

Kakizaki¹,

Ikeda²,

Fukushima³

et al. 2015

OJMS

View full text Add to dashboard Cite

Chitinolytic activities were measured in two fish species having different feeding habits, chub mackerel (Scomber japonicus) and silver croaker (Pennahia argentata). Chitinase (an endo-type chitinolytic enzyme) activity was measured using pNP-(GlcNAc)n (n = 2, 3) as substrates; its level was significantly high in the stomachs of both species, as well as in the gills, intestine, pyloric appendage, testis, and liver of chub mackerel and in the spleen, kidney, pyloric appendage, ovaries, heart, and liver of silver croaker. β-N-Acetylhexosaminidase (an exo-type chitinolytic enzyme) activity was measured using pNP-(GlcNAc) as a substrate; it was detected at high levels in many parts apart from the digestive tracts of both species. The optimum pH for chitinase activity was 3.0 -5.0 in the stomachs of both species, 4.0 in the liver of chub mackerel, and 4.0 and 8.0 in the kidney of silver croaker. Full-length cDNAs encoding two chitinase isozymes were obtained from the stomachs of the two fish species: SjChi-1 (1604 bp) and SjChi-2 (1512 bp) from chub mackerel and PaChi-1 (1630 bp) and PaChi-2 (1606 bp) from silver croaker. Expression analysis of these genes in the organs of the two species revealed strong expression of SjChi-1 in the stomach of chub mackerel and that of PaChi-1 and PaChi-2 in the stomach of silver croaker. The difference in the expression pattern of these genes is likely attributed to the difference in the feeding habits of the two fish species. Our results suggested the presence of novel chitinases in the two species.

show abstract

Section: Introductionmentioning

confidence: 99%

Distribution of Chitinolytic Enzymes in the Organs and cDNA Cloning of Chitinase Isozymes from the Stomach of Two Species of Fish, Chub Mackerel (Scomber japonicus) and Silver Croaker (Pennahia argentata)

Kakizaki¹,

Ikeda²,

Fukushima³

et al. 2015

OJMS

View full text Add to dashboard Cite

show abstract

“…Additionally, chitinase was expressed throughout the life span of Chirostoma estor, with maximum expression in the hepatopancreas of juvenile stage [53]. In zebrafish, chitinase was stably detectable in early embryonic development stage [54], indicating the critical roles of chitinases in innate immunity functioned early than previously assumed. Remarkably, the expression level of chitinase was specifically up-regulated in skin in the resistant pink salmon compared to susceptible fish, and identified as one of the most highly up-regulated genes in juvenile pink salmon during salmon lice infection [55].…”

Section: Expression Profiles Of Chitinase Genes Following Bacterial Cmentioning

confidence: 80%

Characterization and expression analysis of chitinase genes (CHIT1, CHIT2 and CHIT3) in turbot (Scophthalmus maximus L.) following bacterial challenge

Gao

Cai

Zhang

et al. 2017

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Chitinases are hydrolytic enzymes which have been employed to breakdown chitin coats of pathogenic microorganisms, thereby weaken the defense system of several pathogens and insects. In this regard, we identified the chitinase genes of turbot and characterized their expression patterns in mucosal tissues following Vibrio anguillarum and Streptococcus iniae challenge. In present study, transcripts of three chitinase genes (CHIT1, CHIT2 and CHIT3) were captured, as well as their protein structures and expression patterns following different bacterial infection were also characterized. The chitinases were widely expressed in all tested tissues with the highest expression levels of CHIT1 and CHIT2 in intestine, and CHIT3 in skin. Finally, these three genes showed different expression patterns following bacterial challenge. The significant quick induction of chitinases in mucosal surfaces against infection indicated their key roles to prevent pathogen attachment and entry in mucosal immunity. Functional studies should further characterize the chitinases and avail utilization of their function to increase the disease resistance in maintaining the integrity of the mucosal barriers against infection and facilitating the disease resistant family/strain selection in turbot.

show abstract

“…Some of the aforementoioned plant antifungal proteins have counterparts produced by animals and human (Crain and Clark 1985;Barbault et al 2003;Clemons et al 2011;Ng et al 2013a, b;De Bernardis et al 2014;Teng et al 2014;Ueda et al 2014) although there are differences in the detailed structures between the same type of plant and animal/human antifungal proteins. Of course, there are antifungal proteins unique to animals/humans such as cathelicidins and lactoferrin /lactoferricin and other antifungal proteins produced by plants only such as thaumatin-like antifungal proteins and embryo abundant proteins.…”

Section: Other Antifungal Proteinsmentioning

confidence: 98%

Plant antifungal proteins and their applications in agriculture

Yan

Yuan

Jiang

et al. 2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Fungi are far more complex organisms than viruses or bacteria and can develop numerous diseases in plants that cause loss of a substantial portion of the crop every year. Plants have developed various mechanisms to defend themselves against these fungi which include the production of low-molecular-weight secondary metabolites and proteins and peptides with antifungal activity. In this review, families of plant antifungal proteins (AFPs) including defensins, lectins, and several others will be summarized. Moreover, the application of AFPs in agriculture will also be analyzed.

show abstract

Functional characterization of chitinase-3 reveals involvement of chitinases in early embryo immunity in zebrafish

Cited by 23 publications

References 45 publications

Distribution of Chitinolytic Enzymes in the Organs and cDNA Cloning of Chitinase Isozymes from the Stomach of Two Species of Fish, Chub Mackerel (<i>Scomber japonicus</i>) and Silver Croaker (<i>Pennahia argentata</i>)

Distribution of Chitinolytic Enzymes in the Organs and cDNA Cloning of Chitinase Isozymes from the Stomach of Two Species of Fish, Chub Mackerel (<i>Scomber japonicus</i>) and Silver Croaker (<i>Pennahia argentata</i>)

Characterization and expression analysis of chitinase genes (CHIT1, CHIT2 and CHIT3) in turbot (Scophthalmus maximus L.) following bacterial challenge

Plant antifungal proteins and their applications in agriculture

Contact Info

Product

Resources

About

Functional characterization of chitinase-3 reveals involvement of chitinases in early embryo immunity in zebrafish

Cited by 23 publications

References 45 publications

Distribution of Chitinolytic Enzymes in the Organs and cDNA Cloning of Chitinase Isozymes from the Stomach of Two Species of Fish, Chub Mackerel (&lt;i&gt;Scomber japonicus&lt;/i&gt;) and Silver Croaker (&lt;i&gt;Pennahia argentata&lt;/i&gt;)

Distribution of Chitinolytic Enzymes in the Organs and cDNA Cloning of Chitinase Isozymes from the Stomach of Two Species of Fish, Chub Mackerel (&lt;i&gt;Scomber japonicus&lt;/i&gt;) and Silver Croaker (&lt;i&gt;Pennahia argentata&lt;/i&gt;)

Characterization and expression analysis of chitinase genes (CHIT1, CHIT2 and CHIT3) in turbot (Scophthalmus maximus L.) following bacterial challenge

Plant antifungal proteins and their applications in agriculture

Contact Info

Product

Resources

About

Distribution of Chitinolytic Enzymes in the Organs and cDNA Cloning of Chitinase Isozymes from the Stomach of Two Species of Fish, Chub Mackerel (<i>Scomber japonicus</i>) and Silver Croaker (<i>Pennahia argentata</i>)

Distribution of Chitinolytic Enzymes in the Organs and cDNA Cloning of Chitinase Isozymes from the Stomach of Two Species of Fish, Chub Mackerel (<i>Scomber japonicus</i>) and Silver Croaker (<i>Pennahia argentata</i>)