Functional characterization of gibberellin oxidases from cucumber, Cucumis sativus L.

Lange, Maria João Pimenta; Liebrandt, Anja; Arnold, Linda; Chmielewska, Sara-Miriam; Felsberger, André; Freier, Eduard; Heuer, Monika; Zur, D.; Lange, Theodor

doi:10.1016/j.phytochem.2013.02.006

Cited by 50 publications

(35 citation statements)

References 33 publications

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“…GA20ox enzymes catalyze several reactions in the GA biosynthetic pathway to produce precursors that are further converted into bioactive GAs by subsequent enzymes in the pathway ( Figure 6A). We produced recombinant FveGA20ox4 and DFvega20ox4 proteins and showed that, while wild-type FveGA20ox4 converted [ 14 C] GA 12 to [ 14 C] GA 9 ( Figure 6B), as do most other GA20ox enzymes analyzed to date (Pimenta Lange et al, 2013), the mutated DFvega20ox4 enzyme was not able to convert the GA 12 substrate, indicating that the deleted version of the protein was not functional ( Figure 6C). …”

Section: Fvega20ox4 Is Strongly Expressed In the Axmmentioning

confidence: 99%

“…Recombinant protein production, enzyme assays, and enzyme product purification and analysis were performed essentially as described previously (Lange, 1997;Pimenta Lange et al, 2013). The identities of substrate and products were confirmed as their methyl esters trimethylsilyl ethers by gas chromatography-mass spectrometry by comparing their mass spectra with published spectra (Gaskin and MacMillan, 1992): [1-,7-, 12-,18-14 …”

Section: Overexpression In Arabidopsis and Enzymatic Activity Assaysmentioning

confidence: 99%

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A Specific Gibberellin 20-Oxidase Dictates the Flowering-Runnering Decision in Diploid Strawberry

et al. 2017

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Asexual and sexual reproduction occur jointly in many angiosperms. Stolons (elongated stems) are used for asexual reproduction in the crop species potato (Solanum tuberosum) and strawberry (Fragaria spp), where they produce tubers and clonal plants, respectively. In strawberry, stolon production is essential for vegetative propagation at the expense of fruit yield, but the underlying molecular mechanisms are unknown. Here, we show that the stolon deficiency trait of the runnerless (r) natural mutant in woodland diploid strawberry (Fragaria vesca) is due to a deletion in the active site of a gibberellin 20-oxidase (GA20ox) gene, which is expressed primarily in the axillary meristem dome and primordia and in developing stolons. This mutation, which is found in all r mutants, goes back more than three centuries. When FveGA20ox4 is mutated, axillary meristems remain dormant or produce secondary shoots terminated by inflorescences, thus increasing the number of inflorescences in the plant. The application of bioactive gibberellin (GA) restored the runnering phenotype in the r mutant, indicating that GA biosynthesis in the axillary meristem is essential for inducing stolon differentiation. The possibility of regulating the runnering-flowering decision in strawberry via FveGA20ox4 provides a path for improving productivity in strawberry by controlling the trade-off between sexual reproduction and vegetative propagation.

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Section: Fvega20ox4 Is Strongly Expressed In the Axmmentioning

confidence: 99%

Section: Overexpression In Arabidopsis and Enzymatic Activity Assaysmentioning

confidence: 99%

A Specific Gibberellin 20-Oxidase Dictates the Flowering-Runnering Decision in Diploid Strawberry

et al. 2017

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“…C 20 -GA2ox proteins have so far been characterized in Arabidopsis , spinach, and cucumber (Schomburg et al , 2003; Lee and Zeevaart, 2005; Pimenta Lange et al , 2013). According to Lee and Zeevaart (2005), the Arabidopsis C 20 -GA2ox proteins possess a characteristic domain absent in C 19 - GA2ox.…”

Section: Introductionmentioning

confidence: 99%

Gibberellin metabolism in Vitis vinifera L. during bloom and fruit-set: functional characterization and evolution of grapevine gibberellin oxidases

Giacomelli

Rota‐Stabelli

Masuero

et al. 2013

Journal of Experimental Botany

104

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Gibberellins (GAs) are involved in the regulation of flowering and fruit-set in grapes (Vitis vinifera L.), but the molecular mechanisms behind this process are mostly unknown. In this work, the family of grapevine GA oxidases involved in the biosynthesis and deactivation of GAs was characterized. Six putative GA 20-oxidase (GA20ox), three GA 3-oxidase (GA3ox), and eight GA 2-oxidase (GA2ox) proteins, the latter further divided into five C19-GA 2ox and three C20-GA2ox proteins, were identified. Phylogenetic analyses suggest a common origin of the GA3ox and C19-GA2ox groups and challenge previous evolutionary models. In vitro analysis revealed that all GA3ox and GA20ox enzymes prefer substrates of the non-13-hydroxylation pathway. In addition, ectopic expression of GA2ox genes in Arabidopsis thaliana confirmed the activity of their encoded proteins in vivo. The results show that bioactive GA1 accumulates in opening grapevine flowers, whereas at later developmental stages only GA4 is detected in the setting fruit. By studying the expression pattern of the grapevine GA oxidase genes in different organs, and at different stages of flowering and fruit-set, it is proposed that the pool of bioactive GAs is controlled by a fine regulation of the abundance and localization of GA oxidase transcripts.

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“…So far, GA20ox has been isolated from many plants including Arabidopsis thaliana [5], tomato [6], cucumber [7] and tree peony [8]. And it was found that overexpression or downexpression of GA20ox produced different effects on plant growth and development.…”

Section: Introductionmentioning

confidence: 99%

Expression Vector Construction and Genetic Transformation of <i>Paeonia lactiflora</i> Gibberellin 20-Oxidase Gene

Han¹,

Li²,

Guo³

et al. 2017

AJPS

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GA20-oxidase (GA20ox) gene encodes a key enzyme in gibberellins (GAs) biosynthesis pathway. Previously, we have cloned a PlGA20ox gene (GeneBank accession number: KU886552) from Paeonia lactiflora. To further reveal its function, we constructed an expression vector in the present study and then transformed it into Arabidopsis thaliana plants by floral dip method. The transgenic plants exhibited an early bolting, increased height and improved vegetative growth. These results provided efficient vector tool and functional information of PlGA20ox for future gene engineering in peony.

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Functional characterization of gibberellin oxidases from cucumber, Cucumis sativus L.

Cited by 50 publications

References 33 publications

A Specific Gibberellin 20-Oxidase Dictates the Flowering-Runnering Decision in Diploid Strawberry

A Specific Gibberellin 20-Oxidase Dictates the Flowering-Runnering Decision in Diploid Strawberry

Gibberellin metabolism in Vitis vinifera L. during bloom and fruit-set: functional characterization and evolution of grapevine gibberellin oxidases

Expression Vector Construction and Genetic Transformation of <i>Paeonia lactiflora</i> Gibberellin 20-Oxidase Gene

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Functional characterization of gibberellin oxidases from cucumber, Cucumis sativus L.

Cited by 50 publications

References 33 publications

A Specific Gibberellin 20-Oxidase Dictates the Flowering-Runnering Decision in Diploid Strawberry

A Specific Gibberellin 20-Oxidase Dictates the Flowering-Runnering Decision in Diploid Strawberry

Gibberellin metabolism in Vitis vinifera L. during bloom and fruit-set: functional characterization and evolution of grapevine gibberellin oxidases

Expression Vector Construction and Genetic Transformation of &lt;i&gt;Paeonia lactiflora&lt;/i&gt; Gibberellin 20-Oxidase Gene

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Expression Vector Construction and Genetic Transformation of <i>Paeonia lactiflora</i> Gibberellin 20-Oxidase Gene