Functional characterization of Helicoverpa armigera trehalase and investigation of physiological effects caused due to its inhibition by Validamycin A formulation

Adhav, Anmol; Kokane, Surabhi R.; Joshi, Rakesh S.

doi:10.1016/j.ijbiomac.2018.01.221

Cited by 26 publications

(14 citation statements)

References 37 publications

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“…At present, the availability of a large set of genomic data from many insect species (Yin et al, 2015) makes possible genomic analyses suggesting that: (i) treh genes frequently experienced gene duplication during insect evolution; (ii) duplicated copies (paralogs) have been retained in several species bringing to treh gene family (Kalf and Rieder, 1958; Duve, 1972; Huang et al, 2006; Tang et al, 2008, 2012; Chen et al, 2010; Santos et al, 2012; Adhav et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The Evolutionary History and Functional Divergence of Trehalase (treh) Genes in Insects

et al. 2019

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Trehalases ( treh ) have been found in different organisms, such as bacteria, fungi, yeast, nematodes, insects, vertebrates, and plants. Their biochemical properties are extremely variable and not yet fully understood. Gene expression patterns have shown differences among insect species suggesting a potential functional diversification of trehalase enzymes during their evolution. A second gene family encoding for enzymes with hypothetical trehalase activity has been repeatedly annotated in insect genome as acid trehalases/acid trehalase-like ( ath ), but its functional role is still not clear. The currently available large amount of genomic data from many insect species may enable a better understanding of the evolutionary history, phylogenetic relationships and possible roles of trehalase encoding genes in this taxon. The aim of the present study is to infer the evolutionary history of trehalases and acid trehalase genes in insects and analyze the trehalase functional divergence during their evolution, combining phylogenetic and genomic synteny/colinearity analyses.

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Section: Introductionmentioning

confidence: 99%

The Evolutionary History and Functional Divergence of Trehalase (treh) Genes in Insects

et al. 2019

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“…The signi cant decrease in the number of validamycin-treated, viruliferous white ies preferring healthy tomato plants, was probably caused by an insu cient energy supply to ight muscles. It has been demonstrated in various insects that trehalase inhibition can result in increased mortality, developmental abnormalities, reduced ight capacity and diminished fecundity (Adhav et al 2018). For example, injection of trehalase inhibitors such as validamycin A and trehazolin into insect larvae resulted in the failure of pupation of locusts and lethal metamorphosis of adults (Wegener et al 2003(Wegener et al & 2010.…”

Section: Discussionmentioning

confidence: 99%

Validamycin Inhibits The Transmission of Tomato Chlorotic Virus By Bemisia Tabaci

Yang

Shi

Zhang

et al. 2021

Preprint

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Bemisia tabaci (Gennadius), commonly known as whitefly or sweet potato whitefly, causes feeding-related injuries to plants, and transmits more than 200 different plant viruses, including Tomato chlorosis virus (ToCV). Control of B. tabaci is therefore one of the key measures in the comprehensive prevention and control of ToCV outbreak in tomato fields. Many insects rely on the hydrolysis of trehalose, carried out by the enzyme trehalase, to power their flight and other life-sustaining activities. B. tabaci encodes just one trehalase, making it an attractive target. In this study, the mechanism underlying the involvement of trehalase in the transmission of ToCV by B. tabaci was investigated. Also, the effect of the trehalase inhibitor, validamycin, on ToCV transmission was assessed. Our results showed that trehalase activity was upregulated in B. tabaci fed on ToCV-infected tomato plants. Treating B. tabaci with validamycin decreased the trehalase activity, and significantly reduced its transmission of ToCV. Validamycin treatment also inhibited the flying and feeding ability of B. tabaci. These results indicate that proper function of trehalase is required by whitefly to transmit ToCV with high efficiency. They also provide an important theoretical basis for targeting whitefly trehalase as a means to control ToCV transmission.

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“…A similar inhibitory effect of val. A, as well as of other trehalase inhibitors, has been reported in different insects 3,6,7,10–12 …”

Section: Effect Of Validamycin a On Insect Physiologymentioning

confidence: 95%

“…1(A)). It has been demonstrated in various insects that trehalase inhibition can lead to increased mortality, developmental abnormalities, reduced flight capacity and diminished fecundity 10 …”

Section: Effect Of Validamycin a On Insect Physiologymentioning

confidence: 99%

Trehalase inhibition by validamycin A may be a promising target to design new fungicides and insecticides

García

Argüelles

2021

Pest Management Science

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The introduction of insecticides and fungicides in agriculture has improved crop yields and, consequently, the quality of life for many people, especially in what is widely considered as the ‘first world’. However, the indiscriminate use of dangerous chemical insecticides has led to pest resistance, human and animal poisoning and environmental pollution. Biochemical and genetic evidence concludes that the non‐reducing disaccharide trehalose plays an essential role in the pathobiology of many insects and fungi. Both organisms share identical pathway for trehalose biosynthesis (the TPS/TPP pathway), while a high degree of homology in their trehalose hydrolysis capacity (trehalase activities) has also been demonstrated. In the search for new, effective and environmentally sustainable compounds, a set of trehalase inhibitors has emerged as a potentially interesting antifungal and insecticidal target. In particular, the trehalose analogue, Validamycin A, which has a strong inhibitory effect on several trehalases, has been successfully introduced for the treatment of various diseases caused by insects and fungi. Herein, we review the main features of the specific interaction between Validamycin A and trehalase as well as the expected advantages of the applications based on trehalase inhibition as insecticides and fungicides. © 2021 Society of Chemical Industry.

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Functional characterization of Helicoverpa armigera trehalase and investigation of physiological effects caused due to its inhibition by Validamycin A formulation

Cited by 26 publications

References 37 publications

The Evolutionary History and Functional Divergence of Trehalase (treh) Genes in Insects

The Evolutionary History and Functional Divergence of Trehalase (treh) Genes in Insects

Validamycin Inhibits The Transmission of Tomato Chlorotic Virus By Bemisia Tabaci

Trehalase inhibition by validamycin A may be a promising target to design new fungicides and insecticides

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