2017
DOI: 10.1007/s00792-017-0949-8
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Functional characterization of hormone sensitive-like lipase from Bacillus halodurans: synthesis and recovery of pNP-laurate with high yields

Abstract: The HSL-like lipase encoding gene (Blip) from the polyextremophile Bacillus halodurans C-125 has been heterologously expressed in E.coli BL21(DE3). The enzyme is a monomer of ~42 kDa. It has extremely high thermal stability with a t of 35 min at 100 °C. Thermal denaturation/renaturation studies by CD and fluorescence analysis revealed complete refolding of the protein back to its native conformation even after 30 min at 90 °C. Blip prefers substrates with mid to long chain fatty acids. It has a higher catalyti… Show more

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Cited by 9 publications
(5 citation statements)
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“…CR-53 [38], Rheinheimera sp. [39], Lactobacillus plantarum WCFS1 [40,41], and Bacillus halodurans [45]. Furthermore, many bHSL genes were identified through metagenomic approaches directly from environmental DNA samples [46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63][64].…”
Section: Bacterial Hormone-sensitive Lipasesmentioning
confidence: 99%
See 1 more Smart Citation
“…CR-53 [38], Rheinheimera sp. [39], Lactobacillus plantarum WCFS1 [40,41], and Bacillus halodurans [45]. Furthermore, many bHSL genes were identified through metagenomic approaches directly from environmental DNA samples [46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63][64].…”
Section: Bacterial Hormone-sensitive Lipasesmentioning
confidence: 99%
“…Most of the bHSL family members function optimally at mesophilic temperatures, although a systematic approach was carried out to identify HSL genes from glacier soil [95]. However, Blip from Bacillus halodurans C-125 showed high thermal stability with a t 1 / 2 of 35 min at 100°C, and complete refolding to its native conformation after 30 min at 90°C was observed [45]. In addition, EstE1 and EST2 were active at high temperatures of up to 95°C [96,97].…”
Section: Biotechnological Perspectivesmentioning
confidence: 99%
“…While using triacylglycerides olive oil as a substrate, no significant activity could be detected for TLip enzyme, suggesting that TLip corresponds to esterase. As many microbial esterases display broad substrate selectivity (aminolysis, acylation) and high specificity (steroselectivity, regioselectivity, and enantioselectivity) , further detailed work on the investigation of substrate specificity, chiral‐ and regio‐selectivity of TLip would be much interesting. Michaelis–Menten kinetics analysis of TLip using pNPD (C 12 ) as a substrate revealed the catalytic efficiency ( K cat / K m ) was 1.19±0.04 mM −1 Min −1 .…”
Section: Resultsmentioning
confidence: 99%
“…R02, was activated by urea, but inhibited by Triton X-100 and SDS, with 50% maximum activity observed at an SDS concentration of 0.5% (w/v); PMSF had no significant impact on the enzyme activity ( Castilla et al, 2016 ). Finally, a lipase derived from Bacillus halodurans remains relatively stable in commercial detergents and is not affected by EDTA, Triton X-100 and Tween 80; however it is activated by para-Chloromethyl benzoic acid (pCMBA)and 5,5′-dithio-bis-[2-nitrobenzoic acid] (DTNB) and inhibited by diethylpyrocarbonate (DEPC), PMSF, 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDAC)and Woodward’s Reagent K (WRK; Dua and Gupta, 2017 ).…”
Section: Identification Of Lipase Activity In Microorganisms and Analysis Of Its Enzymatic Propertiesmentioning
confidence: 99%