Functional characterization of <scp>CCR</scp> in birch (Betula platyphylla × Betula pendula) through overexpression and suppression analysis

Zhang, Wenbo; Wei, Rui; Chen, Su; Jiang, Jing; Li, Huiyu; Huang, Hongyan; Yang, Guang; Wang, Shuo; Wei, Hairong

doi:10.1111/ppl.12306

Cited by 30 publications

(20 citation statements)

References 71 publications

(108 reference statements)

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“…8). Similar results have been reported in birch, where overexpression of BpCCR1 increased the lignin content of transgenic plants to 14.6% and increased the cell wall thickness of xylem vessel cells [65]. However, overexpression of PbCCR2 did not play a significant role in these indicators, perhaps because PbCCR2 showed low catalytic activity, so it could not cause significant changes in lignin content.…”

Section: Discussionsupporting

confidence: 83%

Transcriptomic analysis of early fruit development in Chinese white pear (Pyrus bretschneideri Rehd.) and functional identification of PbCCR1 in lignin biosynthesis

Zhao

Wang

et al. 2019

BMC Plant Biol

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Background The content of stone cells and lignin is one of the key factors affecting the quality of pear fruit. In a previous study, we determined the developmental regularity of stone cells and lignin in ‘Dangshan Su’ pear fruit 15-145 days after pollination (DAP). However, the development of fruit stone cells and lignin before 15 DAP has not been heavily researched. Results In this study, we found that primordial stone cells began to appear at 7 DAP and that the fruit had formed a large number of stone cells at 15 DAP. Subsequently, transcriptome sequencing was performed on fruits at 0, 7, and 15 DAP and identified 3834 (0 vs. 7 DAP), 4049 (7 vs. 15 DAP) and 5763 (0 vs. 15 DAP) DEGs. During the 7-15 DAP period, a large number of key enzyme genes essential for lignin biosynthesis are gradually up-regulated, and their expression pattern is consistent with the accumulation of lignin in this period. Further analysis found that the biosynthesis of S-type lignin in ‘Dangshan Su’ pear does not depend on the catalytic activity of PbSAD but is primarily generated by the catalytic activity of caffeoyl-CoA through CCoAOMT, CCR, F5H, and CAD. We cloned PbCCR1, 2 and analysed their functions in Chinese white pear lignin biosynthesis. PbCCR1 and 2 have a degree of functional redundancy; both demonstrate the ability to participate in lignin biosynthesis. However, PbCCR1 may be the major gene for lignin biosynthesis, while PbCCR2 has little effect on lignin biosynthesis. Conclusions Our results revealed that ‘Dangshan Su’ pear began to form a large number of stone cells and produce lignin after 7 DAP and mainly accumulated materials from 0 to 7 DAP. PbCCR1 is mainly involved in the biosynthesis of lignin in ‘Dangshan Su’ pear and plays a positive role in lignin biosynthesis.

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Section: Discussionsupporting

confidence: 83%

Transcriptomic analysis of early fruit development in Chinese white pear (Pyrus bretschneideri Rehd.) and functional identification of PbCCR1 in lignin biosynthesis

Zhao

Wang

et al. 2019

BMC Plant Biol

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“…The yl mutant displayed a distinct yellow-green phenotype, while the leaves of all the other BpCCR1-overexpressing lines were a normal color, the same as the wild type birch (WT). One of the BpCCR1-overexpressing lines, C11 with green leaves, was used as control birch [8]. The yl mutant is a very valuable resource because a yellow-green leaf is one of the most popular traits in landscape greening.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Transcriptome Analysis of a Yellow-Green Leaf Mutant in Birch (Betula platyphylla × B. Pendula)

Gang

Chen

Jiang

2019

Forests

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Chlorophyll (Chl)-deficient mutants are ideal materials for the study of Chl biosynthesis, chloroplast development, and photosynthesis. Although the genes encoding key enzymes related to Chl biosynthesis have been well-characterized in herbaceous plants, rice (Oryza sativa L.), Arabidopsis (Arabidopsis thaliana), and maize (Zea mays L.), yellow-green leaf mutants have not yet been fully studied in tree species. In this work, we explored the molecular mechanism of the leaf color formation in a yellow-green leaf mutant (yl). We investigated the differentially expressed genes (DEGs) between yl and control plants (wild type birch (WT) and BpCCR1 overexpression line 11, (C11)) by transcriptome sequencing. Approximately 1163 genes (874 down-regulated and 289 up-regulated) and 930 genes (755 down-regulated and 175 up-regulated) were found to be differentially expressed in yl compared with WT and C11, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis for DEGs revealed that photosynthesis antenna proteins represent the most significant enriched pathway. The expressions of photosynthesis antenna proteins are crucial to the leaf color formation in yl. We also found that Chl accumulate, leaf anatomical structure, photosynthesis, and growth were affected in yl. Taken together, our results not only provide the difference of phenomenal, physiological, and gene expression characteristics in leaves between yl mutant and control plants, but also provide a new insight into the mutation underlying the chlorotic leaf phenotype in birch.

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“…In our previous study, we isolated a T-DNA yellow-green mutant yl form 19 BpCCR1 (cinnamoyl-CoA reductase) overexpression transgenic lines [25]. We used the wild type birch and another BpCCR1 transgenic line C11 as the control plants (Fig.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive characterization of T-DNA integration induced chromosomal rearrangement in a birch T-DNA mutant

et al. 2019

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Background Integration of T-DNA into plant genomes via Agrobacterium may interrupt gene structure and generate numerous mutants. The T-DNA caused mutants are valuable materials for understanding T-DNA integration model in plant research. T-DNA integration in plants is complex and still largely unknown. In this work, we reported that multiple T-DNA fragments caused chromosomal translocation and deletion in a birch ( Betula platyphylla × B. pendula ) T-DNA mutant yl . Results We performed PacBio genome resequencing for yl and the result revealed that two ends of a T-DNA can be integrated into plant genome independently because the two ends can be linked to different chromosomes and cause chromosomal translocation. We also found that these T-DNA were connected into tandem fragment regardless of direction before integrating into plant genome. In addition, the integration of T-DNA in yl genome also caused several chromosomal fragments deletion. We then summarized three cases for T-DNA integration model in the yl genome. (1) A T-DNA fragment is linked to the two ends of a double-stranded break (DSB); (2) Only one end of a T-DNA fragment is linked to a DSB; (3) A T-DNA fragment is linked to the ends of different DSBs. All the observations in the yl genome supported the DSB repair model. Conclusions In this study, we showed a comprehensive genome analysis of a T-DNA mutant and provide a new insight into T-DNA integration in plants. These findings would be helpful for the analysis of T-DNA mutants with special phenotypes. Electronic supplementary material The online version of this article (10.1186/s12864-019-5636-y) contains supplementary material, which is available to authorized users.

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Functional characterization of CCR in birch (Betula platyphylla × Betula pendula) through overexpression and suppression analysis

Cited by 30 publications

References 71 publications

Transcriptomic analysis of early fruit development in Chinese white pear (Pyrus bretschneideri Rehd.) and functional identification of PbCCR1 in lignin biosynthesis

Transcriptomic analysis of early fruit development in Chinese white pear (Pyrus bretschneideri Rehd.) and functional identification of PbCCR1 in lignin biosynthesis

Physiological and Transcriptome Analysis of a Yellow-Green Leaf Mutant in Birch (Betula platyphylla × B. Pendula)

Comprehensive characterization of T-DNA integration induced chromosomal rearrangement in a birch T-DNA mutant

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