Functional characterization of IgM+ B cells and adaptive immunity in lumpfish (Cyclopterus lumpus L.)

Rønneseth, Anita; Ghebretnsae, Dawit B.; Wergeland, Heidrun I.; Haugland, Gyri Teien

doi:10.1016/j.dci.2015.05.010

Cited by 62 publications

(36 citation statements)

References 39 publications

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“…a) and shown a significant role for phagocytic B cells in their innate immunity, suggesting that vaccination will likely confer protection against some infectious pathogens (Rønneseth et al . ). The development of vaccines for certified, disease‐free production of juveniles is thus a research priority and some recent progress has been made with autologous vaccines (Cockerill & Wallis ; Rønneseth et al .…”

Section: Disease Managementmentioning

confidence: 97%

Use of lumpfish for sea‐lice control in salmon farming: challenges and opportunities

Powell

Treasurer²,

Pooley

et al. 2017

Reviews in Aquaculture

216

248

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Efficient sea-lice control remains one of the most important challenges for the salmon farming industry. The use of wrasse (Labridae) as cleaner fish offers an alternative to medicines for sea-lice control, but wrasse tend to become inactive in winter. Lumpfish (Cyclopterus lumpus) continue to feed on sea-lice at low temperatures, and commercial production has escalated from thousands of fish in 2010 to well over 30 million juveniles deployed in 2016. However, production still relies on the capture of wild broodstock, which may not be sustainable. To meet global industry needs, lumpfish production needs to increase to reach c. 50 million fish annually and this can only come from aquaculture. We review current production methods and the use of lumpfish in sea cages and identify some of the main challenges and bottlenecks facing lumpfish intensification. Our gap analysis indicates that the areas in most need of research include better control of maturation for year-round production; formulation of appropriate diets; artificial selection of elite lines with desirable traits; and development of vaccines for certified, disease-free juvenile production. The welfare of farmed lumpfish also needs to be better quantified, and more information is needed on optimal densities and tank design. Finally, the risk of farmed lumpfish escaping from net pens needs to be critically assessed, and we argue that it might be beneficial to recover cleaner fish from salmon cages after the production cycle, perhaps using them as broodstock, for export to the Asian food markets or for the production of animal feeds.

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Section: Disease Managementmentioning

confidence: 97%

Use of lumpfish for sea‐lice control in salmon farming: challenges and opportunities

Powell

Treasurer²,

Pooley

et al. 2017

Reviews in Aquaculture

216

248

View full text Add to dashboard Cite

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“…45 In support of this, the same study found that B cells poorly engulfed large bacteria, 44 whose internalization more likely relies on active phagocytosis. 45 As the phagocytic potential in teleost fish varies between different species and greatly depends on the anatomic source of B cells as well as the nature of the ingested particles, 42,[46][47][48][49] it could be hypothesized that zebrafish B cell populations from different compartments have distinct phagocytic capabilities. On the other hand, it is also possible that this feature was lost in zebrafish in the course of evolution, in line with the specific characteristics that separate many bony fish.…”

Section: Finally Our Work Sheds New Lights On B Cell Immunity In Zebmentioning

confidence: 99%

Themacrophage-expressed gene(mpeg)1identifies a subpopulation of B cells in the adult zebrafish

Ferrero

Gomez

lyer

et al. 2020

Journal of Leukocyte Biology

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The mononuclear phagocytic system consists of many cells, in particular macrophages, scattered throughout the body. However, there is increasing evidence for the heterogeneity of tissue‐resident macrophages, leading to a pressing need for new tools to discriminate mononuclear phagocytic system subsets from other hematopoietic lineages. Macrophage‐expressed gene (Mpeg)1.1 is an evolutionary conserved gene encoding perforin‐2, a pore‐forming protein associated with host defense against pathogens. Zebrafish mpeg1.1:GFP and mpeg1.1:mCherry reporters were originally established to specifically label macrophages. Since then more than 100 peer‐reviewed publications have made use of mpeg1.1‐driven transgenics for in vivo studies, providing new insights into key aspects of macrophage ontogeny, activation, and function. Whereas the macrophage‐specific expression pattern of the mpeg1.1 promoter has been firmly established in the zebrafish embryo, it is currently not known whether this specificity is maintained through adulthood. Here we report direct evidence that beside macrophages, a subpopulation of B‐lymphocytes is marked by mpeg1.1 reporters in most adult zebrafish organs. These mpeg1.1+ lymphoid cells endogenously express mpeg1.1 and can be separated from mpeg1.1+ macrophages by virtue of their light‐scatter characteristics using FACS. Remarkably, our analyses also revealed that B‐lymphocytes, rather than mononuclear phagocytes, constitute the main mpeg1.1‐positive population in irf8null myeloid‐defective mutants, which were previously reported to recover tissue‐resident macrophages in adulthood. One notable exception is skin macrophages, whose development and maintenance appear to be independent from irf8, similar to mammals. Collectively, our findings demonstrate that irf8 functions in myelopoiesis are evolutionary conserved and highlight the need for alternative macrophage‐specific markers to study the mononuclear phagocytic system in adult zebrafish.

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“…The importance of B cells is recognized by their ability to become antibody-secreting cells (ASC), producing immunoglobulins that will target potential pathogens. In addition, B cells of teleost fish have been reported to exhibit additional immunological functions such as phagocytosis and the ability to kill engulfed bacteria (Øverland et al, 2010; Li et al, 2006; Nagasawa et al, 2014; Rønneseth et al, 2015; Zhang et al, 2010). Since mammalian phagocytic B-1 B cells of mammals have a high capacity to present particulate antigen to T cells, the possibility exists that phagocytic B cells of fish play a similar role.…”

Section: Mucosal B Cell Responses In Gutmentioning

confidence: 99%

“…Thus, phagocytic B cells have been described in rainbow trout, Atlantic salmon, Atlantic cod, ginbuna carp, flounder and lump fish (Øverland et al, 2010; Li et al, 2006; Nagasawa et al, 2014; Rønneseth et al, 2015; Zhang et al, 2010). Also, teleost B cells produce a large quantity of polyreactive antibodies (natural antibodies) implicated in several immune functions (Magnadottir, 2006; Magnadottir et al, 2009).…”

Section: Rainbow Trout As a Model For Intestinal Immune Responsesmentioning

confidence: 99%

B cells and their role in the teleost gut

Parra

Korytář

Takizawa

et al. 2016

Developmental & Comparative Immunology

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Mucosal surfaces are the main route of entry for pathogens in all living organisms. In the case of teleost fish, mucosal surfaces cover the vast majority of the animal. As these surfaces are in constant contact with the environment, fish are perpetually exposed to a vast number of pathogens. Despite the potential prevalence and variety of pathogens, mucosal surfaces are primarily populated by commensal non-pathogenic bacteria. Indeed, a fine balance between these two populations of microorganisms is crucial for animal survival. This equilibrium, controlled by the mucosal immune system, maintains homeostasis at mucosal tissues. Teleost fish possess a diffuse mucosa-associated immune system in the intestine, with B cells being one of the main responders. Immunoglobulins produced by these lymphocytes are a critical line of defense against pathogens and also prevent the entrance of commensal bacteria into the epithelium. In this review we will summarize recent literature regarding the role of B-lymphocytes and immunoglobulins in gut immunity in teleost fish, with specific focus on immunoglobulin isotypes and the microorganisms, pathogenic and non-pathogenic that interact with the immune system.

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Functional characterization of IgM+ B cells and adaptive immunity in lumpfish (Cyclopterus lumpus L.)

Cited by 62 publications

References 39 publications

Use of lumpfish for sea‐lice control in salmon farming: challenges and opportunities

Use of lumpfish for sea‐lice control in salmon farming: challenges and opportunities

Themacrophage-expressed gene(mpeg)1identifies a subpopulation of B cells in the adult zebrafish

B cells and their role in the teleost gut

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