Functional Characterization of the Monogalactosyldiacylglycerol Synthase Gene ptMGD2 in the Diatom Phaeodactylum tricornutum

Shang, Shuo; Liu, Ruyi; Luo, Ling; Li, Xitong; Zhang, Shengqiang; Zhang, Yi; Zheng, Peng; Chen, Zhuo; Wang, Baoshan

doi:10.3389/fmars.2022.874448

Cited by 3 publications

(3 citation statements)

References 67 publications

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“…Among them are three phosphorylcholine phosphatases ( PEPC ), which play a role in the absorption of CO 2 in the atmosphere during the photosynthetic process of C4 and asparaginic acid metabolism [ 34 , 35 ]. In addition, there were two MGD2 , Glyma.13G162600 and Glyma.17G108700 , which could be involved in galactolipids biosynthetic pathway and the photosynthetic membranes contain large amounts of galactoselipid MGDG [ 36 , 37 ]. It was speculated that Glyma.13G162600 and Glyma.17G108700 would be involved in the MGDG signaling pathway and photosynthesis of soybean.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analyses Reveal the Key Genes Related to Shade Tolerance in Soybean

Jiang,

Liu,

Gao

et al. 2023

IJMS

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Soybean (Glycine max) is an important crop, rich in proteins, vegetable oils and several other phytochemicals, which is often affected by light during growth. However, the specific regulatory mechanisms of leaf development under shade conditions have yet to be understood. In this study, the transcriptome and metabolome sequencing of leaves from the shade-tolerant soybean ‘Nanxiadou 25′ under natural light (ND1) and 50% shade rate (SHND1) were carried out, respectively. A total of 265 differentially expressed genes (DEGs) were identified, including 144 down-regulated and 121 up-regulated genes. Meanwhile, KEGG enrichment analysis of DEGs was performed and 22 DEGs were significantly enriched in the top five pathways, including histidine metabolism, riboflavin metabolism, vitamin B6 metabolism, glycerolipid metabolism and cutin, suberine and wax biosynthesis. Among all the enrichment pathways, the most DEGs were enriched in plant hormone signaling pathways with 19 DEGs being enriched. Transcription factors were screened out and 34 differentially expressed TFs (DETFs) were identified. Weighted gene co-expression network analysis (WGCNA) was performed and identified 10 core hub genes. Combined analysis of transcriptome and metabolome screened out 36 DEGs, and 12 potential candidate genes were screened out and validated by quantitative real-time polymerase chain reaction (qRT-PCR) assay, which may be related to the mechanism of shade tolerance in soybean, such as ATP phosphoribosyl transferase (ATP-PRT2), phosphocholine phosphatase (PEPC), AUXIN-RESPONSIVE PROTEIN (IAA17), PURPLE ACID PHOSPHATASE (PAP), etc. Our results provide new knowledge for the identification and function of candidate genes regulating soybean shade tolerance and provide valuable resources for the genetic dissection of soybean shade tolerance molecular breeding.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analyses Reveal the Key Genes Related to Shade Tolerance in Soybean

Jiang,

Liu,

Gao

et al. 2023

IJMS

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“…The LACS mutation influenced algal growth and TAG content and altered FA profiles in galactoglycerolipids and phosphatidylcholine (PC), revealing the functions of LACS isozymes in the lipid metabolic process of oleaginous diatom [ 200 ]. The use of CRISPR/Cas gene editing unveiled the regulatory role of MGDG synthase ( MGD ) in the synthesis of monogalactosyl diacylglycerol (MGDG), the most abundant polar lipid in the thylakoid membrane, in P. tricornutum as the knockout of MGD resulted in decreases in MGDG and DGDG (synthesized from MGDG) contents in P. tricornutum [ 201 ]. The lipid accumulation could form lipid droplets (LDs) in microalgae, and the size and structure of LD were related to the LD protein ( LDP ) [ 202 ].…”

Section: Crispr/cas In Marine Algal Researchmentioning

confidence: 99%

“…Acyl-ACP D9-desaturase; long-chain acyl-CoA synthetases (LACS); monogalactosyldiacylglycerol synthase (MGD); stramenopile-type lipid droplet protein (StLDP); NR; glutamine synthetase 2 (GS2); chloroplast localized glutamate synthase(cGOGAT); AP; a novel gene Pt2015 [41,168,198,200,201,203,204,207,208] Others…”

Section: Chlamydomonas Reinhardtiimentioning

confidence: 99%

Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects

Li,

Wu,

Zhang

et al. 2024

Microorganisms

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Initially discovered over 35 years ago in the bacterium Escherichia coli as a defense system against invasion of viral (or other exogenous) DNA into the genome, CRISPR/Cas has ushered in a new era of functional genetics and served as a versatile genetic tool in all branches of life science. CRISPR/Cas has revolutionized the methodology of gene knockout with simplicity and rapidity, but it is also powerful for gene knock-in and gene modification. In the field of marine biology and ecology, this tool has been instrumental in the functional characterization of ‘dark’ genes and the documentation of the functional differentiation of gene paralogs. Powerful as it is, challenges exist that have hindered the advances in functional genetics in some important lineages. This review examines the status of applications of CRISPR/Cas in marine research and assesses the prospect of quickly expanding the deployment of this powerful tool to address the myriad fundamental marine biology and biological oceanography questions.

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Monogalactosyldiacylglycerol synthase isoforms play diverse roles inside and outside the diatom plastid

Guéguen,

Sérès,

Cicéron

et al. 2024

The Plant Cell

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Diatoms derive from a secondary endosymbiosis event, which occurred when a eukaryotic host cell engulfed a red alga. This led to the formation of a complex plastid enclosed by four membranes: two innermost membranes originating from the red alga chloroplast envelope, and two additional peri- and epiplastidial membranes (PPM, EpM). The EpM is linked to the endoplasmic reticulum (ER). The most abundant membrane lipid in diatoms is monogalactosyldiacylglycerol (MGDG), synthesized by galactosyltransferases called MGDG synthases (MGDs), conserved in photosynthetic eukaryotes and considered to be specific to chloroplast membranes. Similar to angiosperms, a multigenic family of MGDs has evolved in diatoms, but through an independent process. We characterized MGDα, MGDβ and MGDγ in Phaeodactylum tricornutum, combining molecular analyses, heterologous expression in Saccharomyces cerevisiae, and studying overexpressing and CRISPR-Cas9-edited lines. MGDα localizes mainly to thylakoids, MGDβ to the PPM, and MGDγ to the ER and EpM. MGDs have distinct specificities for diacylglycerol, consistent with their localization. Results suggest that MGDα is required for thylakoid expansion under optimal conditions, while MGDβ and MGDγ play roles in plastid and non-plastid membranes and in response to environmental stress. Functional compensation among MGDs likely contributes to diatom resilience under adverse conditions and to their ecological success.

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Functional Characterization of the Monogalactosyldiacylglycerol Synthase Gene ptMGD2 in the Diatom Phaeodactylum tricornutum

Cited by 3 publications

References 67 publications

Transcriptomic and Metabolomic Analyses Reveal the Key Genes Related to Shade Tolerance in Soybean

Transcriptomic and Metabolomic Analyses Reveal the Key Genes Related to Shade Tolerance in Soybean

Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects

Monogalactosyldiacylglycerol synthase isoforms play diverse roles inside and outside the diatom plastid

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