Functional characterization of the rice root Germin-like protein gene-1 (OsRGLP1) promoter in Nicotiana tabacum

Abstract

Germin (GER) and germin-like protein (GLP) genes play a very important role against various stresses. Promoter analysis provide significant insight into gene's function and regulation. Presently, upstream region (1228 bp) of the OsRGLP1 gene was functionally characterized via heterologous expression. It was fused with the Glucuronidase (GUS) reporter gene and the expression cassette was used to transform Nicotiana tabacum using Agrobacterium-mediated transformation. Transgenic plants were examined via quantitative real-time PCR (qPCR) to analyze its role in wounding, salinity, drought, abscisic acid (ABA) and circadian rhythm. OsRGLP1 was highly induced by ABA and drought by showing 28-and 25-fold changes in GUS mRNA level respectively as compared to wounding (fourfold change) and salinity (threefold change). However, no activity was observed in circadian rhythm. Histochemically, strong GUS activity was observed in leaf veins, midrib, epidermal hair, stomata guard cells, stem cortex, root hairs, xylem and phloem and at cellular level in cell wall, cytoplasm and its periphery. OsRGLP1 promoter can be used to develop agronomically important transgenic plants in future food program. KeywordsRice germin-like proteins • OsRGLP1 • Germin • Germin-like protein • Promoter • GUS • Stress * Muhammad Ilyas

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

“… 93 Similarly, the GUS gene controlled by the OsRGLP1 promoter showed strong activity in the root when tested against drought, ABA, wounding, and salinity. 94 A similar result was also noted for the OsRGLP2 promoter. 95 , 96 In the same way, the JA-mediated role of OsGER4 in crown root development was also confirmed in rice.…”

“…The study revealed that they regulate various plant processes mainly in the root and maturation zones, which is per the prominent role of GER and GLPs in roots. ,, Previously, a germin-like oxalate oxidase activity was detected in the primary root (epidermal cells) and coleorhiza of barley seedlings . Similarly, the GUS gene controlled by the OsRGLP1 promoter showed strong activity in the root when tested against drought, ABA, wounding, and salinity . A similar result was also noted for the OsRGLP2 promoter. , In the same way, the JA-mediated role of OsGER4 in crown root development was also confirmed in rice .…”

Genome-wide analysis of the GLP gene family and overexpression of GLP1-5–1 to promote lignin accumulation during early somatic embryo development in Dimocarpus longan

“…GLP genes can as act as SOD, OXO and serine protease inhibitors to participate in plant growth and development [14]. SOD activity was significantly increased after overexpression of DlGLP1-5-1, which was consistent with the result showing that GLP can act as SOD to participate in plant growth and development.…”

“…GLPs are widely expressed in different organs, such as roots, stems, leaves, flowers and seeds; they also have diverse functions in different plant developmental stages [12,13] and are mainly involved in a variety of physiological processes in the form of receptors, enzymes and structural proteins [14]. Previous studies have shown that PsGLP1 and PsGLP2 are involved in the whole process of flower and fruit development in different varieties of plum (Prunus) [15].…”

Analysis of Germin-like protein genes family in Vitis vinifera (VvGLPs) using various in silico approaches

“…Sacc. and Alternaria solani Sorauer] stresses (Ilyas et al, 2019;Munir et al, 2016). However, it is contradictory to the study of Mahmood et al, (2010) where ARA, W-box, GT-element and ACGT Sequence were common to 10 GLPs promoters of 5 plant species.…”