Functional characterization of the three genes encoding 1-deoxy-D-xylulose 5-phosphate synthase in maize

Córdoba, Elizabeth; Porta, Helena; Arroyo, Analilia; Román, Carolina San; Medina, L.; Rodríguez‐Concepción, Manuel; León, Patricia

doi:10.1093/jxb/erq393

Cited by 125 publications

(108 citation statements)

References 70 publications

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“…S5; Walter et al, 2002;Phillips et al, 2007). Some plant species have a third DXS protein cluster referred to as the DXS3 clade, which has been proposed to participate in the synthesis of some products essential for plant survival (Cordoba et al, 2009(Cordoba et al, , 2011. We found that SrDXS3 was a member of the DXS3 clade (Fig.…”

Section: Expression and Molecular Analysis Of The Mep Pathway And Idimentioning

confidence: 75%

Comparative transcriptomics unravel biochemical specialization of leaf tissues of Stevia (Stevia rebaudiana) for diterpenoid production

et al. 2015

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Stevia (Stevia rebaudiana) produces not only a group of diterpenoid glycosides known as steviol glycosides (SGs), but also other labdane-type diterpenoids that may be spatially separated from SGs. However, their biosynthetic routes and spatial distribution in leaf tissues have not yet been elucidated. Here, we integrate metabolome and transcriptome analyses of Stevia to explore the biosynthetic capacity of leaf tissues for diterpenoid metabolism. Tissue-specific chemical analyses confirmed that SGs were accumulated in leaf cells but not in trichomes. On the other hand, Stevia leaf trichomes stored other labdane-type diterpenoids such as oxomanoyl oxide and agatholic acid. RNA sequencing analyses from two different tissues of Stevia provided a comprehensive overview of dynamic metabolic activities in trichomes and leaf without trichomes. These metabolite-guided transcriptomics and phylogenetic and gene expression analyses clearly identified specific gene members encoding enzymes involved in the 2-C-methyl-D-erythritol 4-phosphate pathway and the biosynthesis of steviol or other labdane-type diterpenoids. Additionally, our RNA sequencing analysis uncovered copalyl diphosphate synthase (SrCPS) and kaurene synthase1 (SrKS1) homologs, SrCPS2 and KS-like (SrKSL), which were specifically expressed in trichomes. In vitro and in planta assays showed that unlike SrCPS and SrKS1, SrCPS2 synthesized labda-13-en-8-ol diphosphate and successively catalyzed the formation of manoyl oxide and epi-manoyl oxide in combination with SrKSL. Our findings suggest that Stevia may have evolved to use distinct metabolic pathways to avoid metabolic interferences in leaf tissues for efficient production of diverse secondary metabolites.

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Section: Expression and Molecular Analysis Of The Mep Pathway And Idimentioning

confidence: 75%

Comparative transcriptomics unravel biochemical specialization of leaf tissues of Stevia (Stevia rebaudiana) for diterpenoid production

et al. 2015

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“…DXS1 is a limiting factor in upregulation of the carotenoid pathway in A. thaliana 49 . DXS1 expression is induced by light 50,51 , and it is the main DXS isoform catalyzing the biosynthesis of isoprenoid and carotenoid precursors in photosynthetic metabolism 52,53 . DXS1 also regulates carotenoid accumulation in A. thaliana and tomato 54,55 .…”

Section: A Model For Carotenoid Accumulation In Carrot Rootsmentioning

confidence: 99%

A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution

et al. 2016

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5 7Carrot (Daucus carota subsp. carota L.; 2n = 2x = 18) is a globally important root crop whose production has quadrupled between 1976 and 2013 (FAO Statistics; see URLs), outpacing the overall rate of increase in vegetable production and world population growth (FAO Statistics; see URLs) through development of high-value products for fresh consumption, juices, and natural pigments and cultivars adapted to warmer production regions 1 .The first documented colors for domesticated carrot root were yellow and purple in Central Asia approximately 1,100 years ago 2,3 , with orange carrots not reliably reported until the sixteenth century in Europe 4,5 . The popularity of orange carrots is fortuitous for modern consumers because the orange pigmentation results from high quantities of alpha-and beta-carotene, making carrots the richest source of provitamin A in the US diet 6 . Carrot breeding has substantially increased nutritional value, with a 50% average increase in carotene content in the United States as compared to 40 years ago 6 . Lycopene and lutein in red and yellow carrots, respectively, are also nutritionally important carotenoids, making carrot a model system to study storage root development and carotenoid accumulation.Carrot is the most important crop in the Apiaceae family, which includes numerous other vegetables, herbs, spices, and medicinal plants that enhance the epicurean experience 7 , including celery, parsnip, arracacha, parsley, fennel, coriander, and cumin. The Apiaceae family belongs to the euasterid II clade, which includes important crops such as lettuce and sunflower 8 . Genome sequences of euasterid I species have been reported, but only two genomes 9,10 have been published among the other euasterid II species.Here we report a high-quality genome assembly of a doubledhaploid orange carrot, characterization of the mechanism controlling carotenoid accumulation in storage roots, and the resequencing of 35 accessions spanning the genetic diversity of the Daucus genus. Our comprehensive genomic analyses provide insights into the evolution of the asterids and several gene families. These results will facilitate biological discovery and crop improvement in carrot and other crops.A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution We report a high-quality chromosome-scale assembly and analysis of the carrot (Daucus carota) genome, the first sequenced genome to include a comparative evolutionary analysis among members of the euasterid II clade. We characterized two new polyploidization events, both occurring after the divergence of carrot from members of the Asterales order, clarifying the evolutionary scenario before and after radiation of the two main asterid clades. Large- and small-scale lineage-specific duplications have contributed to the expansion of gene families, including those with roles in flowering time, defense response, flavor, and pigment accumulation. We identified a candidate gene, DCAR_032551, that conditions caro...

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“…5:1 mixture of IPP and DMAPP (Figure 1). Unlike Arabidopsis, most plants appear to have small gene families encoding DXS isoforms of at least two classes (Walter et al, 2002;Krushkal et al, 2003;Kim et al, 2005;Cordoba et al, 2009;Paetzold et al, 2010;Cordoba et al, 2011). All classes of DXS isozymes have been found to participate in carotenoid biosynthesis.…”

Section: Mep Pathway To Ggppmentioning

confidence: 99%

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

Ruiz-Sola

Rodríguez‐Concepción

2012

The Arabidopsis Book

475

372

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Plant carotenoids are a family of pigments that participate in light harvesting and are essential for photoprotection against excess light. Furthermore, they act as precursors for the production of apocarotenoid hormones such as abscisic acid and strigolactones. In this review, we summarize the current knowledge on the genes and enzymes of the carotenoid biosynthetic pathway (which is now almost completely elucidated) and on the regulation of carotenoid biosynthesis at both transcriptional and post-transcriptional levels. We also discuss the relevance of Arabidopsis as a model system for the study of carotenogenesis and how metabolic engineering approaches in this plant have taught important lessons for carotenoid biotechnology.

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Functional characterization of the three genes encoding 1-deoxy-D-xylulose 5-phosphate synthase in maize

Cited by 125 publications

References 70 publications

Comparative transcriptomics unravel biochemical specialization of leaf tissues of Stevia (Stevia rebaudiana) for diterpenoid production

Comparative transcriptomics unravel biochemical specialization of leaf tissues of Stevia (Stevia rebaudiana) for diterpenoid production

A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

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