Functional chemical modification of a porcine acellular dermal matrix with a modified naturally derived polysaccharide crosslinker

Dan, Nianhua; Huang, Yanping; Bai, Zhongxiang; Yang, Changkai; Dan, Weihua; Cong, Liangliang

doi:10.1002/app.47633

Cited by 16 publications

(20 citation statements)

References 35 publications

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“…Unlike glutaraldehyde or formaldehyde, OCOS is a derivative of chitosan oligosaccharide, retained a skeleton structure like the COS, so it could avoid some harmful influences of aldehyde groups. This kind of phenomenon can be summed up as a “compensation mechanism,” which is one of the greatest advantages of a naturally derived crosslinker . When compared with oxidized chitosan, OCOS has a smaller molecular weight, better water solubility, smaller steric hindrance, and better permeability, which might achieve better performance.…”

Section: Resultsmentioning

confidence: 99%

Insights into the interactions between collagen and a naturally derived crosslinker, oxidized chitosan oligosaccharide

Dan

Huang

Yang

et al. 2019

J of Applied Polymer Sci

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Collagen has been widely applied to biomaterials. However, it must be chemically fixed before it can be used. The aim of this work is to study on the crosslinking effect of a naturally derived oxidized chitosan oligosaccharide (OCOS) on collagen type I. The physicochemical properties and biocompatibility of the crosslinked collagen (OCOS-Col) were evaluated. The FTIR spectroscopy showed that crosslinking via OCOS had no impact on the structural integrity of collagen. DSC and TG tests showed that the thermal stability of crosslinked collagen was improved significantly, while the crosslinking density test indicated that the amino groups of collagen could predominantly react with the available aldehyde groups of OCOS. The mechanical properties, hydrophilicity, and enzymatic degradation test showed that the crosslinked material had improved properties. However, the porosity test showed that the crosslinked material was more compact, which was consistent with the AFM observation that crosslinked collagen revealed a denser network structure. Cytotoxicity test showed that the crosslinked collagen was conducive to cell adhesion, growth, and proliferation. In conclusion, this work reveals that OCOS stabilized collagen as a crosslinker, preserved its triple helical structure, and reserved its good biocompatibility. OCOS was proven to be a safe and reliable crosslinker for collagen.

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Section: Resultsmentioning

confidence: 99%

Insights into the interactions between collagen and a naturally derived crosslinker, oxidized chitosan oligosaccharide

Dan

Huang

Yang

et al. 2019

J of Applied Polymer Sci

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“… 61 The live bacteria number of the control group was designated as N 0 and that of the samples was designated as N sample . The antibacterial rates of the materials were calculated by the following equation 63 …”

Section: Methodsmentioning

confidence: 99%

Preparation and Characterization of Antibacterial Porcine Acellular Dermal Matrices with High Performance

Wang

Gong

et al. 2020

ACS Omega

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Infection is a common complication in the process of wound management. An ideal wound dressing is supposed to reduce or even prevent the infection while promoting wound healing. A porcine acellular dermal matrix (pADM) has been already used as a wound dressing in clinic due to its capacity to accelerate wound healing. However, not only is pure pADM not antibacterial, its mechanical properties are poor. In this study, an antibacterial pADM with good performance was prepared by adding two natural products as modifiers, quercetin (QCT) and tea tree oil (TTO). The result of Fourier-transform infrared (FTIR) proved that the addition of modifiers did not break the natural triple-helical structure of collagen. Meanwhile, the results of differential scanning calorimetry (DSC), thermogravimetric analysis (TG), mechanic experiment, and enzymatic degradation demonstrated that pADM handled with QCT and TTO (termed QCT–TTO–pADM) had better thermal stability, mechanical strength, and resistance to enzymatic degradation than pADM. Meanwhile, QCT–TTO–pADM had excellent antibacterial activity and showed an antibacterial rate of over 80%. Furthermore, in the cytocompatibility analysis, QCT–TTO–pADM had no side effects on the adhesion, growth, and proliferation of fibroblasts. QCT–TTO–pADM could even accelerate wound healing more efficiently than pADM and glutaraldehyde-modified pADM (GA-pADM). In conclusion, QCT–TTO–pADM was a potential antibacterial wound dressing with good performance.

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“…These properties make them promising candidates for crosslinking wound healing biomaterials. For instance, after crosslinking ADM with oxidized 2-hydroxypropyl trimethylammonium chloride chitosan (OHTCC), the obtained scaffolds possessed better physicochemical characteristics, including improved tensile strength, enhanced antibacterial activity, and better enzymatic stability [75]. To further avoid the possible cytotoxicity of OHTCC, Zheng et al have synthesized epoxidized N-(2-hydroxypropyl)-3trimethylammonium chitosan chloride (EHTCC) and successfully produced an EHTCCcrosslinked ADM, which showed not only improved mechanical properties, thermal sta-bility, and hydrophilicity but also excellent cellular compatibility and wound healing capacity [76].…”

Section: Chemical Crosslinkingmentioning

confidence: 99%

Membranous Extracellular Matrix-Based Scaffolds for Skin Wound Healing

Huang

Xie

et al. 2021

Pharmaceutics

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Membranous extracellular matrix (ECM)-based scaffolds are one of the most promising biomaterials for skin wound healing, some of which, such as acellular dermal matrix, small intestinal submucosa, and amniotic membrane, have been clinically applied to treat chronic wounds with acceptable outcomes. Nevertheless, the wide clinical applications are always hindered by the poor mechanical properties, the uncontrollable degradation, and other factors after implantation. To highlight the feasible strategies to overcome the limitations, in this review, we first outline the current clinical use of traditional membranous ECM scaffolds for skin wound healing and briefly introduce the possible repair mechanisms; then, we discuss their potential limitations and further summarize recent advances in the scaffold modification and fabrication technologies that have been applied to engineer new ECM-based membranes. With the development of scaffold modification approaches, nanotechnology and material manufacturing techniques, various types of advanced ECM-based membranes have been reported in the literature. Importantly, they possess much better properties for skin wound healing, and would become promising candidates for future clinical translation.

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Functional chemical modification of a porcine acellular dermal matrix with a modified naturally derived polysaccharide crosslinker

Cited by 16 publications

References 35 publications

Insights into the interactions between collagen and a naturally derived crosslinker, oxidized chitosan oligosaccharide

Insights into the interactions between collagen and a naturally derived crosslinker, oxidized chitosan oligosaccharide

Preparation and Characterization of Antibacterial Porcine Acellular Dermal Matrices with High Performance

Membranous Extracellular Matrix-Based Scaffolds for Skin Wound Healing

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