Functional cloning of a cDNA encoding Mei2‐like protein from <i>Arabidopsis thaliana</i> using a fission yeast pheromone receptor deficient mutant

Hirayama, Takashi; Ishida, Chika; Kuromori, Takashi; Obata, Seiji; Shimoda, Chikashi; Yamamoto, Masayuki; Shinozaki, Kazuo; Ohto, Chikara

doi:10.1016/s0014-5793(97)00871-5

Cited by 22 publications

(14 citation statements)

References 32 publications

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“…However, it is still possible that WCSP1 is involved in the regulation of cold acclimation-associated genes at the levels of transcription or translation. Increasing numbers of reports are demonstrating the importance of RNA-binding proteins in the regulation of developmental and physiological processes such as flowering time (63), leaf development (64), meiosis (65), and circadian rhythms (66). Thus, it is of great interest to determine the function of WCSP1 in the regulation of the cold acclimation process.…”

Section: Discussionmentioning

confidence: 99%

A Cold-regulated Nucleic Acid-binding Protein of Winter Wheat Shares a Domain with Bacterial Cold Shock Proteins

Karlson

Nakaminami

Toyomasu

et al. 2002

Journal of Biological Chemistry

145

154

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The molecular mechanisms of cold acclimation are still largely unknown; however, it has been established that overwintering plants such as winter wheat increases freeze tolerance during cold treatments. In prokaryotes, cold shock proteins are induced by temperature downshifts and have been proposed to function as RNA chaperones. A wheat cDNA encoding a putative nucleic acid-binding protein, WCSP1, was isolated and found to be homologous to the predominant CspA of Escherichia coli. The putative WCSP1 protein contains a three-domain structure consisting of an N-terminal cold shock domain with two internal conserved consensus RNA binding domains and an internal glycine-rich region, which is interspersed with three C-terminal CX 2 CX 4 HX 4 C (CCHC) zinc fingers. Each domain has been described independently within several nucleotide-binding proteins. Northern and Western blot analyses showed that WCSP1 mRNA and protein levels steadily increased during cold acclimation, respectively. WCSP1 induction was cold-specific because neither abscisic acid treatment, drought, salinity, nor heat stress induced WCSP1 expression. Nucleotide binding assays determined that WCSP1 binds ssDNA, dsDNA, and RNA homopolymers. The capacity to bind dsDNA was nearly eliminated in a mutant protein lacking C-terminal zinc fingers. Structural and expression similarities to E. coli CspA suggest that WCSP1 may be involved in gene regulation during cold acclimation.

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Section: Discussionmentioning

confidence: 99%

A Cold-regulated Nucleic Acid-binding Protein of Winter Wheat Shares a Domain with Bacterial Cold Shock Proteins

Karlson

Nakaminami

Toyomasu

et al. 2002

Journal of Biological Chemistry

145

154

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“…AML1 was isolated in a screen for Arabidopsis cDNAs that could allow meiosis to proceed in a mam2 map3 mutant of S. pombe that is deficient in the mating receptors and hence in the receptor signaling that is required for initiation of meiosis (Hirayama et al, 1997). Although AML1 could rescue the mating receptor signaling requirement for meiosis, it could not complement a mei2 mutant.…”

Section: Introductionmentioning

confidence: 99%

TheArabidopsis-mei2-LikeGenes Play a Role in Meiosis and Vegetative Growth inArabidopsis

2006

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The Arabidopsis-mei2-Like (AML) genes comprise a five-member gene family related to the mei2 gene, which is a master regulator of meiosis in Schizosaccharomyces pombe and encodes an RNA binding protein. We have analyzed the AML genes to assess their role in plant meiosis and development. All five AML genes were expressed in both vegetative and reproductive tissues. Analysis of AML1-AML5 expression at the cellular level indicated a closely similar expression pattern. In the inflorescence, expression was concentrated in the shoot apical meristem, young buds, and reproductive organ primordia. Within the reproductive organs, strong expression was observed in meiocytes and developing gametes. Functional analysis using RNA interference (RNAi) and combinations of insertion alleles revealed a role for the AML genes in meiosis, with RNAi lines and specific multiple mutant combinations displaying sterility and a range of defects in meiotic chromosome behavior. Defects in seedling growth were also observed at low penetrance. These results indicate that the AML genes play a role in meiosis as well as in vegetative growth and reveal conservation in the genetic mechanisms controlling meiosis in yeast and plants.

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“…2). Plants contain two groups, which we refer to as the AML group [AML1-like, after Arabidopsis AML1 (Hirayama et al 1997)] and the TEL group [Terminal ear1-like, after the maize te1 gene (Veit et al 1998)]. The monophyly of the AML and TEL groups are well supported by a Templeton non-parametric test (nonmonophyly of these groups was rejected at p =0.0011 and 0.0004 respectively).…”

Section: Evolutionary History Of Mei2-like Genesmentioning

confidence: 96%

“…Three cloned genes were identified as Mei2-like: S. pombe Mei2 (Shimoda et al 1987), Arabidopsis AML1 (Hirayama et al 1997) and maize te1 (Veit et al 1998). To extend this dataset we searched the Arabidopsis thaliana genome (Arabidopsis Genome Initiative 2000) for more sequences encoding Mei2-like genes by tBLASTn (Altschul et al 1990) using the AML1, te1 and Mei2 protein sequences as queries (see Table 1 for GenBank accessions), accepting hits with E-values <10 -6 .…”

Section: Primary Datasetmentioning

confidence: 99%

“…The fact that these mammalians cells appear to recognise the localisation signal(s) that the Mei2-meiRNA presents supports the idea that mammalian cells once contained a Mei2-like protein. Also, heterologous expression of the Arabidopsis protein AML1 will serve to stimulate meiosis in meiosis-deficient S. pombe cells (showing plant to fungi compatibility; Hirayama et al 1997). Whatever the nature of the developmental switch, it appears to be an ancient eukaryote mechanism.…”

Section: Conservation Of Rrms and Sequence Motifsmentioning

confidence: 99%

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A description of the Mei2-like protein family; structure, phylogenetic distribution and biological context

Jeffares

Phillips

Moore

et al. 2004

Development Genes and Evolution

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The Schizosaccharomyces pombe Mei2 gene encodes an RNA recognition motif (RRM) protein that stimulates meiosis upon binding a specific non-coding RNA and subsequent accumulation in a "mei2-dot" in the nucleus. We present here the first systematic characterization of the family of proteins with characteristic Mei2-like amino acid sequences. Mei2-like proteins are an ancient eukaryotic protein family with three identifiable RRMs. The C-terminal RRM (RRM3) is unique to Mei2-like proteins and is the most highly conserved of the three RRMs. RRM3 also contains conserved sequence elements at its C-terminus not found in other RRM domains. Single copy Mei2-like genes are present in some fungi, in alveolates such as Paramecium and in the early branching eukaryote Entamoeba histolytica, while plants contain small families of Mei2-like genes. While the C-terminal RRM is highly conserved between plants and fungi, indicating conservation of molecular mechanisms, plant Mei2-like genes have changed biological context to regulate various aspects of developmental pattern formation.

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Functional cloning of a cDNA encoding Mei2‐like protein from Arabidopsis thaliana using a fission yeast pheromone receptor deficient mutant

Cited by 22 publications

References 32 publications

A Cold-regulated Nucleic Acid-binding Protein of Winter Wheat Shares a Domain with Bacterial Cold Shock Proteins

A Cold-regulated Nucleic Acid-binding Protein of Winter Wheat Shares a Domain with Bacterial Cold Shock Proteins

TheArabidopsis-mei2-LikeGenes Play a Role in Meiosis and Vegetative Growth inArabidopsis

A description of the Mei2-like protein family; structure, phylogenetic distribution and biological context

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