Functional closure of visceral pleural defects by autologous tissue engineered cell sheets☆

Kanzaki, Masato; Yamato, Masayuki; Yang, Joseph; Sekine, Hidekazu; Takagi, Ryo; Isaka, Tamami; Okano, Teruo; Onuki, Takamasa

doi:10.1016/j.ejcts.2008.05.048

Cited by 53 publications

(31 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study by Kanzaki et al (23) used skin fibroblast cell sheets from rabbits as a novel lung air leak sealant and achieved an immediate and permanent lung air leak seal in the rabbit pulmonary air leakage model. Kanzaki et al (24) examined the effects of autologous dermal fibroblast sheets in the closure of pleural defects in a porcine model. The autologous dermal fibroblast sheets were attached directly to the lung surface, and provided an immediate seal against <25 cm H 2 O of airway pressure.…”

Section: Applications Of Cell Sheet Techniques With Non-mesenchymal Smentioning

confidence: 99%

Application of the cell sheet technique in tissue engineering

Chen

Niu

et al. 2015

Biomedical Reports

View full text Add to dashboard Cite

Section: Applications Of Cell Sheet Techniques With Non-mesenchymal Smentioning

confidence: 99%

Application of the cell sheet technique in tissue engineering

Chen

Niu

et al. 2015

Biomedical Reports

View full text Add to dashboard Cite

“…Clinical studies using autologous cell sheet‐tissues were performed in the fields of ophthalmology, periodontology, cardiovascular medicine, orthopedics, gastrointestinal surgery, and otolaryngology . Moreover, for treating various diseases, including pneumothorax, diabetes mellitus, hepatic disease, hemophilia, renal dysfunction, endocrinopathy, and uterine disorders, various studies using cell sheet‐tissues are ongoing . In clinical and preclinical studies, OCM will be a powerful tool for evaluating cell sheet‐tissues noninvasively, for example, their thickness, cell condition, and adhesions between layered cell sheets.…”

Section: Discussionmentioning

confidence: 99%

“…[41][42][43][44][45][46] Moreover, for treating various diseases, including pneumothorax, diabetes mellitus, hepatic disease, hemophilia, renal dysfunction, endocrinopathy, and uterine disorders, various studies using cell sheet-tissues are ongoing. [47][48][49][50][51][52][53][54] In clinical and preclinical studies, OCM will be a powerful tool for evaluating cell sheet-tissues noninvasively, for example, their thickness, cell condition, and adhesions between layered cell sheets. Due to its noninvasiveness, it is not necessary to fabricate extra tissues for observation, which results in greater cell savings, lower costs, and less preparation times.…”

Section: Discussionmentioning

confidence: 99%

Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section

Hasegawa

Haraguchi

Oikaze

et al. 2015

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Optical coherence tomography (OCT) is a valuable tool in the cross-sectional observation/analysis of three-dimensional (3-D) biological tissues, and that histological observation is important clinically. However, the resolution of the technology is approximately 10-20 μm. In this study, optical coherence microscopy (OCM), a tomographic system combining OCT technology with a microscopic technique, was constructed for observing cells individually with a resolution at the submicrometer level. Cells and 3-D tissues fabricated by cell sheet technology were observed by OCM. Importantly, the cell nuclei and cytoplasm could be clearly distinguished, and the time-dependent dynamics of cell-sheet tissues could be observed in detail. Additionally, the 3-D migration of cells in the bioengineered tissue was also detected using OCM and metal-labeled cells. Bovine aortic endothelial cells, but not NIH3T3 murine embryonic skin fibroblasts, actively migrated within the 3-D tissues. This study showed that the OCM system would be a valuable tool in the fields of cell biology, tissue engineering, and regenerative medicine. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 481-488, 2017.

show abstract

“…In view of a report that transplanting cell sheets fabricated from skin fibroblasts was effective in preventing pulmonary air leaks after respiratory surgery , we devised a way to fabricate stratified cell sheets that have mesothelial cells, which have the ability to prevent intestinal adhesions, in the upper layer, and, peritoneal fibroblast‐derived cell sheets as the base . These cell sheets have the advantage of being able to increase cell sheet adhesiveness as a result of containing fibroblasts that are rich in collagen, fibronectin, etc., in the lower layer, and of being able to more effectively exert the barrier function that the mesothelial cells in the upper layer possess and prevent adhesion between different parts of the intestine.…”

Section: Attempts To Apply Cell Sheet Technology To Peritoneal Regenementioning

confidence: 99%

Therapeutic Applications of Mesothelial Cell Sheets

et al. 2014

Self Cite

View full text Add to dashboard Cite

Mesothelial cells are an integral part of the peritoneum and play an important role in maintaining its structural and functional properties. In recent years a number of studies on mesothelial cells have been performed to evaluate the localization, secretional properties and the ability of regeneration and transdifferentiation of these cells. They are also involved in the repair of the peritoneum damage following surgery or peritonitis. Mesothelial cells produce several cytokines, growth factors and extracellular matrix components, possessing anti-inflammatory and immunomodulatory properties. Based on previous research, cell sheet engineering has made it possible to transplant cells that retain the cells' function, and stacking different cells in layers has also become possible. Arranging blood vessels between the cell layers is a problem when stacking cells in layers. Whether adequate blood flow can be obtained for the cell layers to survive long-term is the difference between success and failure. Mesothelial cell transplantation for peritoneal regeneration needs to be performed under conditions in which the surface area of the visceral peritoneum is large and the mesothelial cell damage area is small. In this article we explain cell sheet engineering as one of the technologies for transplanting cells with a variety of intercellular adhesion and cell membrane molecules maintained intact, and its application to peritoneal regeneration.

show abstract

Functional closure of visceral pleural defects by autologous tissue engineered cell sheets☆

Abstract: This study presents the development of an effective sealant for visceral pleural defects using autologous cells that have the flexibility to respond to expansion and contraction during respiration.

Cited by 53 publications

References 25 publications

Application of the cell sheet technique in tissue engineering

Application of the cell sheet technique in tissue engineering

Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section

Therapeutic Applications of Mesothelial Cell Sheets

Contact Info

Product

Resources

About