1992
DOI: 10.1016/0006-291x(92)91673-e
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Functional co-expression of human oxidoreductase and cytochrome P450 1A1 in Saccharomyces cerevisiae results in increased erod activity

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Cited by 28 publications
(20 citation statements)
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“…The lower eukaryote yeast S.cerevisiae contains an endogenous oxidoreductase (30) which can couple with heterologous CYP enzymes from other organisms. However, from our previous studies of heterologous CYP cDNA expression, it was concluded that the level of yeast oxidoreductase was limiting or that interaction between yeast oxidoreductase and human CYP was suboptimal (18). Here we show for both CYPs from family 1 that co-expression of human oxidoreductase significantly increases their promutagen potency.…”
Section: Discussionmentioning
confidence: 51%
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“…The lower eukaryote yeast S.cerevisiae contains an endogenous oxidoreductase (30) which can couple with heterologous CYP enzymes from other organisms. However, from our previous studies of heterologous CYP cDNA expression, it was concluded that the level of yeast oxidoreductase was limiting or that interaction between yeast oxidoreductase and human CYP was suboptimal (18). Here we show for both CYPs from family 1 that co-expression of human oxidoreductase significantly increases their promutagen potency.…”
Section: Discussionmentioning
confidence: 51%
“…Such crplA strains are viable on normal media but they react supersensitively towards the antifungal drug ketoconazole (24). The supersensitive phenotype is rescued by expression of human oxidoreductase cDNA in such strains (18). YNW64 transformants containing individual plasmids were tested for their sensitivity towards low doses of ketoconazole.…”
Section: Co-expression Of Cytochrome P-450 and Oxidoreductase Cdnas Imentioning
confidence: 99%
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“…The 2 URA3 plasmids pMK637 (this work) or pSB229 (Eugster et al, 1992), containing hCYP1A2ϩhOR and hCYP1A1ϩhOR cDNAs, respectively, or the LEU2 plasmid pCS512 (Sengstag et al, 1996), containing hCYP1A1ϩhOR cDNAs, were first introduced into yeast strains by DNA transformation to metabolically activate the AFB 1 and benzo-(a)-pyrene-7,8-dihydrodiol (BaP-DHD; Klebe et al, 1983). The 2 URA3 plasmid pCS316, containing the hCYP1A1ϩhOR cDNA in the opposite orientation as in pSB229 (Eugster et al, 1992), was introduced into YB110, YB324, and YB335 to measure AFB 1 -associated translocations in mec1 checkpoint mutants.…”
Section: Media and Strainsmentioning
confidence: 99%
“…The 2 URA3 plasmid pCS316, containing the hCYP1A1ϩhOR cDNA in the opposite orientation as in pSB229 (Eugster et al, 1992), was introduced into YB110, YB324, and YB335 to measure AFB 1 -associated translocations in mec1 checkpoint mutants. pMK637 was introduced into the strain YMK2181 to measure AFB 1 related-changes in gene expression using the oligonucleotide arrays.…”
Section: Media and Strainsmentioning
confidence: 99%