Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5

Stackhouse, Murray; Ortiz, Jeanelle B.; Sato, Koki; Chen, David J.

doi:10.1016/0165-7992(94)90044-2

Cited by 10 publications

(1 citation statement)

References 17 publications

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“…Chromosome 5 has been used to complement deficiencies in double-strand break repair in a mutant mouse line sensitive to ionising radiation (Stackhouse et al 1994). XRCC4, a gene important in double-strand break repair, was then discovered by MMCT into Chinese hamster mutant cells to reside on human chromosome 5q15-q12 (Athwal and Kaur 1996).…”

Section: Discovery Of Genes and Chromosomal Regions Involved In Dna Rmentioning

confidence: 99%

The manipulation of chromosomes by mankind: the uses of microcell-mediated chromosome transfer

2005

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Microcell-mediated chromosome transfer (MMCT) was a technique originally developed in the 1970s to transfer exogenous chromosome material into host cells. Although, the methodology has not changed considerably since this time it is being used to great success in progressing several different fields in modern day biology. MMCT is being employed by groups all over the world to hunt for tumour suppressor genes associated with specific cancers, DNA repair genes, senescence-inducing genes and telomerase suppression genes. Some of these genomic discoveries are being investigated as potential treatments for cancer. Other fields have taken advantage of MMCT, and these include assessing genomic stability, genomic imprinting, chromatin modification and structure and spatial genome organisation. MMCT has also been a very useful method in construction and manipulation of artificial chromosomes for potential gene therapies. Indeed, MMCT is used to transfer mainly fragmented mini-chromosome between cell types and into embryonic stem cells for the construction of transgenic animals. This review briefly discusses these various uses and some of the consequences and advancements made by different fields utilising MMCT technology.

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Section: Discovery Of Genes and Chromosomal Regions Involved In Dna Rmentioning

confidence: 99%

The manipulation of chromosomes by mankind: the uses of microcell-mediated chromosome transfer

2005

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Different contributions of the indirect effects of γ-rays on the cytotoxicity in M10 and XRCC4 transfected M10 cells

Miyazaki

Nakano

Ito

et al. 2007

Radiat Environ Biophys

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The protective effects of dimethyl sulfoxide (DMSO) against cell killing by (137)Cs gamma-rays were investigated in XRCC4-deficient cell line M10, XRCC4-complemented M10 and the parental mouse leukemia cell line L5178Y. Cell survival was determined by the colony-forming ability. M10 cells were more sensitive to gamma-ray-induced cell death than L5178Y and complemented M10 cells. Cell survival was increased in both M10 and L5178Y in the presence of DMSO. However, estimation of the DMSO-protectable fraction revealed a smaller protectable fraction for M10 cells than for L5178Y cells, indicating that indirect effects contributed in a smaller extent to the cytotoxicity in M10 than that in L5178Y. This effect is due to XRCC4 deficiency, since transfection of XRCC4 cDNA into M10 cells restored the radioprotective effects of DMSO to the level seen in L5178Y. In M10 cells, the killing effects of high LET radiation (Auger electrons from (125)I-antipyrine, carbon ions with an LET of 166 keV microm(-1)) were similar to those of low LET radiation ((137)Cs gamma-rays, characteristic X-rays from (125)I-bovine serum albumin). We discuss that lethal lesions produced by indirect actions in L5178Y and XRCC4-complemented M10 cells may differ, at least in part, from DNA double-strand breaks repairable by non-homologous end joining.

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Mammalian mutants defective in the response to ionizing radiation-induced DNA damage

Zdzienicka

1995

Mutation Research/DNA Repair

116

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Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5

Cited by 10 publications

References 17 publications

The manipulation of chromosomes by mankind: the uses of microcell-mediated chromosome transfer

The manipulation of chromosomes by mankind: the uses of microcell-mediated chromosome transfer

Different contributions of the indirect effects of γ-rays on the cytotoxicity in M10 and XRCC4 transfected M10 cells

Mammalian mutants defective in the response to ionizing radiation-induced DNA damage

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