Use of benthic macroinvertebrates has been in vogue as indicator organisms for water quality monitoring since long. Traditional methods of water quality monitoring incorporate mostly monitoring of physicochemical parameters. However, it is the biomonitoring studies that could help in more accurate water quality monitoring. Due to anthropogenic impact at water sources, integrated water quality monitoring has become a necessity. An integrated study comprising physicochemical parameters and biomonitoring using benthic macroinvertebrates was carried out at six sites along the Mahi river basin, the command area of which has witnessed tremendous economic and cultural progress over a period of time. Diversity Score and Saprobic Score were calculated, and Water Quality Class was determined as per Biological Monitoring Working Party score given by Central Pollution Control Board and results in moderate pollution at all locations, whereas Water Quality Class as per ISI-IS 2296–1982 at M-1, M-4, M-7 and M-12 shows slight pollution. Jaccard’s similarity index compared the biodiversity of benthic macroinvertebrates at all the sites from upstream to downstream locations. It was maximum, i.e., 52.94% between the locations at M-4 and M-7. Species richness (S) has also been monitored at the said locations. The Diversity Score 0.83 is maximum at the Galteshwar (M-12) location of the Mahi river, which indicates that the location was the most ecologically diverse region among all.